Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection

Autores
Lo Presti, Maria Silvina; Esteves, Blanca H.; Moya, Diego; Bazán, Paola Carolina; Strauss, Mariana; Báez, Alejandra L.; Pizzi, Rogelio; Quispe Recalde, M. Antonieta; Valladares, Basilio; Rivarola, H. Walter; Paglini Oliva, Patricia A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated the presence and distribution of two Trypanosoma cruzi natural isolates in blood, heart, skeletal muscle, liver, and spleen tissues in the acute phase of the experimental infection (35 days postinfection) in order to determine if the populations present in blood were different to those found in the tissues of the same host. Thirty mice were infected with 50 forms of each isolate or with a combination of them. Presence and molecular characterization of the parasites in the host tissues were determined by specific PCR. Cardiac and skeletal muscle alterations were analyzed by histological studies. T. cruzi variability in the host tissues was analyzed through RFLP studies. Both isolates used consisted of a mixture of two T. cruzi lineages. Specific PCRs were positive for most of the samples from the 3 groups analyzed. Cardiac and skeletal muscle sections from the groups infected with one isolate presented mild to moderate inflammatory infiltrates; the group infected with both isolates showed severe inflammatory infiltrates and the presence of amastigote nests in both tissues. Different parasite populations were found in circulation and in the tissues from the same host. These results are important for patients with high probability of mixed infections in endemic areas and contribute to the knowledge of parasite/host interactions.
Fil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Esteves, Blanca H.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Moya, Diego. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Bazán, Paola Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Pizzi, Rogelio. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Quispe Recalde, M. Antonieta. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; España
Fil: Valladares, Basilio. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; España
Fil: Rivarola, H. Walter. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina. Universidad Nacional de la Rioja; Argentina
Fil: Paglini Oliva, Patricia A.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Materia
Trypanosoma Cruzi Lineages or Dtus
Natural Isolates
Tissue Distribution
Acute Phase of Chagas Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11508
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infectionLo Presti, Maria SilvinaEsteves, Blanca H.Moya, DiegoBazán, Paola CarolinaStrauss, MarianaBáez, Alejandra L.Pizzi, RogelioQuispe Recalde, M. AntonietaValladares, BasilioRivarola, H. WalterPaglini Oliva, Patricia A.Trypanosoma Cruzi Lineages or DtusNatural IsolatesTissue DistributionAcute Phase of Chagas Diseasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We evaluated the presence and distribution of two Trypanosoma cruzi natural isolates in blood, heart, skeletal muscle, liver, and spleen tissues in the acute phase of the experimental infection (35 days postinfection) in order to determine if the populations present in blood were different to those found in the tissues of the same host. Thirty mice were infected with 50 forms of each isolate or with a combination of them. Presence and molecular characterization of the parasites in the host tissues were determined by specific PCR. Cardiac and skeletal muscle alterations were analyzed by histological studies. T. cruzi variability in the host tissues was analyzed through RFLP studies. Both isolates used consisted of a mixture of two T. cruzi lineages. Specific PCRs were positive for most of the samples from the 3 groups analyzed. Cardiac and skeletal muscle sections from the groups infected with one isolate presented mild to moderate inflammatory infiltrates; the group infected with both isolates showed severe inflammatory infiltrates and the presence of amastigote nests in both tissues. Different parasite populations were found in circulation and in the tissues from the same host. These results are important for patients with high probability of mixed infections in endemic areas and contribute to the knowledge of parasite/host interactions.Fil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Esteves, Blanca H.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Moya, Diego. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Bazán, Paola Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Pizzi, Rogelio. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Quispe Recalde, M. Antonieta. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; EspañaFil: Valladares, Basilio. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; EspañaFil: Rivarola, H. Walter. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina. Universidad Nacional de la Rioja; ArgentinaFil: Paglini Oliva, Patricia A.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaElsevier Science2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11508Lo Presti, Maria Silvina; Esteves, Blanca H.; Moya, Diego; Bazán, Paola Carolina; Strauss, Mariana; et al.; Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection; Elsevier Science; Acta Tropica; 133; 5-2014; 98-1090001-706Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2014.02.010info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X14000394info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:00Zoai:ri.conicet.gov.ar:11336/11508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:00.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
title Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
spellingShingle Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
Lo Presti, Maria Silvina
Trypanosoma Cruzi Lineages or Dtus
Natural Isolates
Tissue Distribution
Acute Phase of Chagas Disease
title_short Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
title_full Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
title_fullStr Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
title_full_unstemmed Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
title_sort Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection
dc.creator.none.fl_str_mv Lo Presti, Maria Silvina
Esteves, Blanca H.
Moya, Diego
Bazán, Paola Carolina
Strauss, Mariana
Báez, Alejandra L.
Pizzi, Rogelio
Quispe Recalde, M. Antonieta
Valladares, Basilio
Rivarola, H. Walter
Paglini Oliva, Patricia A.
author Lo Presti, Maria Silvina
author_facet Lo Presti, Maria Silvina
Esteves, Blanca H.
Moya, Diego
Bazán, Paola Carolina
Strauss, Mariana
Báez, Alejandra L.
Pizzi, Rogelio
Quispe Recalde, M. Antonieta
Valladares, Basilio
Rivarola, H. Walter
Paglini Oliva, Patricia A.
author_role author
author2 Esteves, Blanca H.
Moya, Diego
Bazán, Paola Carolina
Strauss, Mariana
Báez, Alejandra L.
Pizzi, Rogelio
Quispe Recalde, M. Antonieta
Valladares, Basilio
Rivarola, H. Walter
Paglini Oliva, Patricia A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma Cruzi Lineages or Dtus
Natural Isolates
Tissue Distribution
Acute Phase of Chagas Disease
topic Trypanosoma Cruzi Lineages or Dtus
Natural Isolates
Tissue Distribution
Acute Phase of Chagas Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We evaluated the presence and distribution of two Trypanosoma cruzi natural isolates in blood, heart, skeletal muscle, liver, and spleen tissues in the acute phase of the experimental infection (35 days postinfection) in order to determine if the populations present in blood were different to those found in the tissues of the same host. Thirty mice were infected with 50 forms of each isolate or with a combination of them. Presence and molecular characterization of the parasites in the host tissues were determined by specific PCR. Cardiac and skeletal muscle alterations were analyzed by histological studies. T. cruzi variability in the host tissues was analyzed through RFLP studies. Both isolates used consisted of a mixture of two T. cruzi lineages. Specific PCRs were positive for most of the samples from the 3 groups analyzed. Cardiac and skeletal muscle sections from the groups infected with one isolate presented mild to moderate inflammatory infiltrates; the group infected with both isolates showed severe inflammatory infiltrates and the presence of amastigote nests in both tissues. Different parasite populations were found in circulation and in the tissues from the same host. These results are important for patients with high probability of mixed infections in endemic areas and contribute to the knowledge of parasite/host interactions.
Fil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Esteves, Blanca H.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Moya, Diego. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Bazán, Paola Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Pizzi, Rogelio. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Quispe Recalde, M. Antonieta. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; España
Fil: Valladares, Basilio. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias; España
Fil: Rivarola, H. Walter. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina. Universidad Nacional de la Rioja; Argentina
Fil: Paglini Oliva, Patricia A.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
description We evaluated the presence and distribution of two Trypanosoma cruzi natural isolates in blood, heart, skeletal muscle, liver, and spleen tissues in the acute phase of the experimental infection (35 days postinfection) in order to determine if the populations present in blood were different to those found in the tissues of the same host. Thirty mice were infected with 50 forms of each isolate or with a combination of them. Presence and molecular characterization of the parasites in the host tissues were determined by specific PCR. Cardiac and skeletal muscle alterations were analyzed by histological studies. T. cruzi variability in the host tissues was analyzed through RFLP studies. Both isolates used consisted of a mixture of two T. cruzi lineages. Specific PCRs were positive for most of the samples from the 3 groups analyzed. Cardiac and skeletal muscle sections from the groups infected with one isolate presented mild to moderate inflammatory infiltrates; the group infected with both isolates showed severe inflammatory infiltrates and the presence of amastigote nests in both tissues. Different parasite populations were found in circulation and in the tissues from the same host. These results are important for patients with high probability of mixed infections in endemic areas and contribute to the knowledge of parasite/host interactions.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11508
Lo Presti, Maria Silvina; Esteves, Blanca H.; Moya, Diego; Bazán, Paola Carolina; Strauss, Mariana; et al.; Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection; Elsevier Science; Acta Tropica; 133; 5-2014; 98-109
0001-706X
url http://hdl.handle.net/11336/11508
identifier_str_mv Lo Presti, Maria Silvina; Esteves, Blanca H.; Moya, Diego; Bazán, Paola Carolina; Strauss, Mariana; et al.; Circulating Trypanosoma cruzi populations differ from those found in the tissues of the same host during acute experimental infection; Elsevier Science; Acta Tropica; 133; 5-2014; 98-109
0001-706X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2014.02.010
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X14000394
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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