Expression of genes related with the rna-directed dna methylation (rddm) pathway in Eragrostis curvula sexual and apomictic genotypes

• Autores: Selva, Juan Pablo; Garbus, Ingrid; Romero, José Rodolfo; Pessino, Silvina Claudia; Leblanc, Olivier; Echenique, Carmen Viviana
• Año de publicación: 2014
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: APOMIXIS; METHYLTRANFERASES; RDDM; RNA
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Apomixis in plants refers to a diverse group of developmental behaviors resulting in asexual reproduction through seeds. Apomictic individuals bypass both meiotic reduction and egg cell fertilization to produce offspring that are exact genetic replicas of the maternal plant. Given the established relationships between sexual and apomictic pathways, different models have been proposed to explain the occurrence of apomixis at the molecular level involving genetic and epigenetic mechanisms. Weeping lovegrass [Eragrostis curvula (Schrader) Nees] is an apomictic perennial grass native of Southern Africa. The type of apomixis present in the E. curvula is pseudogamous diplospory. There are recent evidences on the role played by certain proteins of the RdDM pathways involved in gametophytic apomixis expression, suggesting an epigenetic regulation of the trait. It was reported in maize that loss-of-function of the dmt103 and dmt102 methyltransferases genes partially mimics apomictic developments. Similarly, loss-of-function in ago104, a maize homolog of ago9, also results in apomixis-like traits, giving rise to up to 70% of functional unreduced female gametes. These data suggest that an epigenetic regulation plays a role on the establishment of apomixis. In this work we studied the expression of EcDmt102-like and EcAgo104-like genes associated to the RdDM pathway in flowers and leaves of sexual and apomictic E. curvula plants. Using RNA seq data was possible to obtain the complete cDNA sequence for both genes from both apomictic and sexual genotypes. The phylogenetic analysis indicated that these genes group together in the same clade with the corresponding ones of Arabidopsis and Zea mays. The EcDmt102-like and EcAgo104-like genes profiles were analyzed using qRT-PCR in leaves and different stages of reproductive tissue. The expression profile obtained for EcDmt102-like was relatively higher in reproductive tissue of sexual plants, being down-regulated in apomictic ones. Expression of this gene was not observed in leaves from both genotypes. The expression pattern of EcAgo104-like was complex and is still under study because occurrence of a putative alternative splicing in the apomictic genotypes is being analysed. Our results support the reported roles played by this genes in gametophytic apomixis in other plant species.
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Leblanc, Olivier. Université Montpellier II; Francia
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
IV Ciclo de Seminarios sobre avances en la caracterización genética y molecular de la apomixis
Bahía Blanca
Argentina
Universidad Nacional del Sur
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida