Analysing bacterial communities from microbial mats and sediments located in the Atacama desert

Autores
Fernández, Ana Belén; Rasuk, Maria Cecilia; Kurth, Daniel German; Contreras, M.; Novoa, F.; Poire, D.; Farias, Maria Eugenia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Atacama Desert has more than 100 basins with interior drainage and most of them contain salt flats. These ecosystemshave extreme environmental conditions that allow the development of unique microbial communities. The objetctive of this wasto study the bacterial diversity using independent culture tools of microbial mats and sediments from salt flats in the AtacamaDesert. Some physicochemical conditions of the water surrounding these samples were analysed to discover if anyphysicochemical characteristic could be influencing in its taxonomic composition. Five samples were collected, three of themwere microbial mats and two were sediments. The mat samples were taken from Laguna Llamara (samples named LL1 andLL2) and Laguna Cejar (Cej). Sediments were taken from Laguna Jachucoposa (Cop) and Laguna Pujsa (Puj) where microbialmats are not present. Total metagenomic DNA extraction was performed on each sample and the V4 hypervariable region of thebacterial 16S rRNA gene was amplified by pyrosequencing using the Ribosomal Database Project (RDP)-suggested universalprimers. Diversity of the microbial community was assessed using the QIIME software package. Lakes that harbor microbialmats have a higher salinity and a lower dissolved oxygen concentration and proportion of organic matter and total phosphorousthan lakes where mats are absent. All the samples have important concentrations of arsenic, with an extremely high amount inPuj. Proteobacteria and/or Bacteroidetes are the major phyla represented in all samples. Also, other phyla as Spirochaetes,Chloroflexi or Verrucomicrobia are found. However, cyanobacterial sequences are only observed in LL2 and Puj. On the otherhand, we have found a higher diversity in sediment than in mat samples. The sediments samples contain phyla not observed inmat samples. 16S rRNA gene sequences classified within Actinobacteria and Gracilibacteria are only found in Puj and related toTenericutes, Gemmatimonadetes and Acidobacteria are only observed in Cop. Finally, an important fraction of the sequencescould not be classified at phylum level. The high diversity found in sediment samples may be explained by the physicochemicalconditions in the environment. For example, they have a lower conductivity than mat samples. It is known hypersalineenvironments have a low diversity, where halophilic microorganisms are able to survive to these extreme conditions becausethey have specific strategies to balance the osmotic pressure. Besides, we found a low proportion or absence of Cyanobacteriain the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers inthese extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be affiliated to anyknown bacterial phyla suggesting that in these ecosystems there are potential novel representatives of bacterial phyla not yetdescribed.
Fil: Fernández, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Contreras, M.. No especifíca;
Fil: Novoa, F.. No especifíca;
Fil: Poire, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
Materia
MICROBIAL DIVERSITY
ATACAMA DESERT
MICROBIAL MATS
SEDIMENTS
HYPERSALINE LAKES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195145
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195145
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysing bacterial communities from microbial mats and sediments located in the Atacama desertFernández, Ana BelénRasuk, Maria CeciliaKurth, Daniel GermanContreras, M.Novoa, F.Poire, D.Farias, Maria EugeniaMICROBIAL DIVERSITYATACAMA DESERTMICROBIAL MATSSEDIMENTSHYPERSALINE LAKEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Atacama Desert has more than 100 basins with interior drainage and most of them contain salt flats. These ecosystemshave extreme environmental conditions that allow the development of unique microbial communities. The objetctive of this wasto study the bacterial diversity using independent culture tools of microbial mats and sediments from salt flats in the AtacamaDesert. Some physicochemical conditions of the water surrounding these samples were analysed to discover if anyphysicochemical characteristic could be influencing in its taxonomic composition. Five samples were collected, three of themwere microbial mats and two were sediments. The mat samples were taken from Laguna Llamara (samples named LL1 andLL2) and Laguna Cejar (Cej). Sediments were taken from Laguna Jachucoposa (Cop) and Laguna Pujsa (Puj) where microbialmats are not present. Total metagenomic DNA extraction was performed on each sample and the V4 hypervariable region of thebacterial 16S rRNA gene was amplified by pyrosequencing using the Ribosomal Database Project (RDP)-suggested universalprimers. Diversity of the microbial community was assessed using the QIIME software package. Lakes that harbor microbialmats have a higher salinity and a lower dissolved oxygen concentration and proportion of organic matter and total phosphorousthan lakes where mats are absent. All the samples have important concentrations of arsenic, with an extremely high amount inPuj. Proteobacteria and/or Bacteroidetes are the major phyla represented in all samples. Also, other phyla as Spirochaetes,Chloroflexi or Verrucomicrobia are found. However, cyanobacterial sequences are only observed in LL2 and Puj. On the otherhand, we have found a higher diversity in sediment than in mat samples. The sediments samples contain phyla not observed inmat samples. 16S rRNA gene sequences classified within Actinobacteria and Gracilibacteria are only found in Puj and related toTenericutes, Gemmatimonadetes and Acidobacteria are only observed in Cop. Finally, an important fraction of the sequencescould not be classified at phylum level. The high diversity found in sediment samples may be explained by the physicochemicalconditions in the environment. For example, they have a lower conductivity than mat samples. It is known hypersalineenvironments have a low diversity, where halophilic microorganisms are able to survive to these extreme conditions becausethey have specific strategies to balance the osmotic pressure. Besides, we found a low proportion or absence of Cyanobacteriain the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers inthese extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be affiliated to anyknown bacterial phyla suggesting that in these ecosystems there are potential novel representatives of bacterial phyla not yetdescribed.Fil: Fernández, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Contreras, M.. No especifíca;Fil: Novoa, F.. No especifíca;Fil: Poire, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXI Congreso Argentino de Microbiología GeneralCórdobaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195145Analysing bacterial communities from microbial mats and sediments located in the Atacama desert; XI Congreso Argentino de Microbiología General ; Córdoba; Argentina; 2015; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/doc/2015/Libro%20de%20Resumenes%20FINAL.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:28Zoai:ri.conicet.gov.ar:11336/195145instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:28.47CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
title Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
spellingShingle Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
Fernández, Ana Belén
MICROBIAL DIVERSITY
ATACAMA DESERT
MICROBIAL MATS
SEDIMENTS
HYPERSALINE LAKES
title_short Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
title_full Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
title_fullStr Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
title_full_unstemmed Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
title_sort Analysing bacterial communities from microbial mats and sediments located in the Atacama desert
dc.creator.none.fl_str_mv Fernández, Ana Belén
Rasuk, Maria Cecilia
Kurth, Daniel German
Contreras, M.
Novoa, F.
Poire, D.
Farias, Maria Eugenia
author Fernández, Ana Belén
author_facet Fernández, Ana Belén
Rasuk, Maria Cecilia
Kurth, Daniel German
Contreras, M.
Novoa, F.
Poire, D.
Farias, Maria Eugenia
author_role author
author2 Rasuk, Maria Cecilia
Kurth, Daniel German
Contreras, M.
Novoa, F.
Poire, D.
Farias, Maria Eugenia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROBIAL DIVERSITY
ATACAMA DESERT
MICROBIAL MATS
SEDIMENTS
HYPERSALINE LAKES
topic MICROBIAL DIVERSITY
ATACAMA DESERT
MICROBIAL MATS
SEDIMENTS
HYPERSALINE LAKES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Atacama Desert has more than 100 basins with interior drainage and most of them contain salt flats. These ecosystemshave extreme environmental conditions that allow the development of unique microbial communities. The objetctive of this wasto study the bacterial diversity using independent culture tools of microbial mats and sediments from salt flats in the AtacamaDesert. Some physicochemical conditions of the water surrounding these samples were analysed to discover if anyphysicochemical characteristic could be influencing in its taxonomic composition. Five samples were collected, three of themwere microbial mats and two were sediments. The mat samples were taken from Laguna Llamara (samples named LL1 andLL2) and Laguna Cejar (Cej). Sediments were taken from Laguna Jachucoposa (Cop) and Laguna Pujsa (Puj) where microbialmats are not present. Total metagenomic DNA extraction was performed on each sample and the V4 hypervariable region of thebacterial 16S rRNA gene was amplified by pyrosequencing using the Ribosomal Database Project (RDP)-suggested universalprimers. Diversity of the microbial community was assessed using the QIIME software package. Lakes that harbor microbialmats have a higher salinity and a lower dissolved oxygen concentration and proportion of organic matter and total phosphorousthan lakes where mats are absent. All the samples have important concentrations of arsenic, with an extremely high amount inPuj. Proteobacteria and/or Bacteroidetes are the major phyla represented in all samples. Also, other phyla as Spirochaetes,Chloroflexi or Verrucomicrobia are found. However, cyanobacterial sequences are only observed in LL2 and Puj. On the otherhand, we have found a higher diversity in sediment than in mat samples. The sediments samples contain phyla not observed inmat samples. 16S rRNA gene sequences classified within Actinobacteria and Gracilibacteria are only found in Puj and related toTenericutes, Gemmatimonadetes and Acidobacteria are only observed in Cop. Finally, an important fraction of the sequencescould not be classified at phylum level. The high diversity found in sediment samples may be explained by the physicochemicalconditions in the environment. For example, they have a lower conductivity than mat samples. It is known hypersalineenvironments have a low diversity, where halophilic microorganisms are able to survive to these extreme conditions becausethey have specific strategies to balance the osmotic pressure. Besides, we found a low proportion or absence of Cyanobacteriain the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers inthese extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be affiliated to anyknown bacterial phyla suggesting that in these ecosystems there are potential novel representatives of bacterial phyla not yetdescribed.
Fil: Fernández, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Contreras, M.. No especifíca;
Fil: Novoa, F.. No especifíca;
Fil: Poire, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
description The Atacama Desert has more than 100 basins with interior drainage and most of them contain salt flats. These ecosystemshave extreme environmental conditions that allow the development of unique microbial communities. The objetctive of this wasto study the bacterial diversity using independent culture tools of microbial mats and sediments from salt flats in the AtacamaDesert. Some physicochemical conditions of the water surrounding these samples were analysed to discover if anyphysicochemical characteristic could be influencing in its taxonomic composition. Five samples were collected, three of themwere microbial mats and two were sediments. The mat samples were taken from Laguna Llamara (samples named LL1 andLL2) and Laguna Cejar (Cej). Sediments were taken from Laguna Jachucoposa (Cop) and Laguna Pujsa (Puj) where microbialmats are not present. Total metagenomic DNA extraction was performed on each sample and the V4 hypervariable region of thebacterial 16S rRNA gene was amplified by pyrosequencing using the Ribosomal Database Project (RDP)-suggested universalprimers. Diversity of the microbial community was assessed using the QIIME software package. Lakes that harbor microbialmats have a higher salinity and a lower dissolved oxygen concentration and proportion of organic matter and total phosphorousthan lakes where mats are absent. All the samples have important concentrations of arsenic, with an extremely high amount inPuj. Proteobacteria and/or Bacteroidetes are the major phyla represented in all samples. Also, other phyla as Spirochaetes,Chloroflexi or Verrucomicrobia are found. However, cyanobacterial sequences are only observed in LL2 and Puj. On the otherhand, we have found a higher diversity in sediment than in mat samples. The sediments samples contain phyla not observed inmat samples. 16S rRNA gene sequences classified within Actinobacteria and Gracilibacteria are only found in Puj and related toTenericutes, Gemmatimonadetes and Acidobacteria are only observed in Cop. Finally, an important fraction of the sequencescould not be classified at phylum level. The high diversity found in sediment samples may be explained by the physicochemicalconditions in the environment. For example, they have a lower conductivity than mat samples. It is known hypersalineenvironments have a low diversity, where halophilic microorganisms are able to survive to these extreme conditions becausethey have specific strategies to balance the osmotic pressure. Besides, we found a low proportion or absence of Cyanobacteriain the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers inthese extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be affiliated to anyknown bacterial phyla suggesting that in these ecosystems there are potential novel representatives of bacterial phyla not yetdescribed.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195145
Analysing bacterial communities from microbial mats and sediments located in the Atacama desert; XI Congreso Argentino de Microbiología General ; Córdoba; Argentina; 2015; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195145
identifier_str_mv Analysing bacterial communities from microbial mats and sediments located in the Atacama desert; XI Congreso Argentino de Microbiología General ; Córdoba; Argentina; 2015; 1-2
CONICET Digital
CONICET
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language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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