Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome

Autores
Ochoa, Federico Claudio; Lago, N. R.; Gerhardt, Elizabeth; Ibarra, Cristina Adriana; Zotta, E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, hemolytic uremic syndrome (HUS) constitutes the most frequent cause of acute renal failure in children. The aim of our study was to analyze the early tubular response under the effect of Shiga toxin type 2 (Stx2) in a rat experimental model of HUS. METHODS: Adult male Sprague-Dawley rats were injected intraperitoneally with culture supernatant from recombinant Escherichia coli expressing Stx2. Functional, histological, immunohistochemical and Western blot studies were performed at 48 h postinoculation. RESULTS: Renal tubules showed the loss of the epithelial markers E-cadherin and beta-catenin, and an increase in transforming growth factor-beta1 expression. We detected the expression of alpha-smooth muscle actin in the interstitium and fibrosis in the periglomerular areas. CONCLUSION: Our results indicate that the early tubular response to the effects of Stx2 is related to an immunophenotype change of tubular cells and the presence of mild fibrosis in the interstitium.
Fil: Ochoa, Federico Claudio. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Lago, N. R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Gerhardt, Elizabeth. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Materia
ACUTE RENAL FAILURE
ALPHA-SMOOTH MUSCLE ACTIN
EPITHELIAL MARKERS
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN
TRANSFORMING GROWTH FACTOR-BETA1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198389
Acceder
id CONICETDig_70ab584af132be67e770b5e31761aa78
oai_identifier_str oai:ri.conicet.gov.ar:11336/198389
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndromeOchoa, Federico ClaudioLago, N. R.Gerhardt, ElizabethIbarra, Cristina AdrianaZotta, E.ACUTE RENAL FAILUREALPHA-SMOOTH MUSCLE ACTINEPITHELIAL MARKERSHEMOLYTIC UREMIC SYNDROMESHIGA TOXINTRANSFORMING GROWTH FACTOR-BETA1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In Argentina, hemolytic uremic syndrome (HUS) constitutes the most frequent cause of acute renal failure in children. The aim of our study was to analyze the early tubular response under the effect of Shiga toxin type 2 (Stx2) in a rat experimental model of HUS. METHODS: Adult male Sprague-Dawley rats were injected intraperitoneally with culture supernatant from recombinant Escherichia coli expressing Stx2. Functional, histological, immunohistochemical and Western blot studies were performed at 48 h postinoculation. RESULTS: Renal tubules showed the loss of the epithelial markers E-cadherin and beta-catenin, and an increase in transforming growth factor-beta1 expression. We detected the expression of alpha-smooth muscle actin in the interstitium and fibrosis in the periglomerular areas. CONCLUSION: Our results indicate that the early tubular response to the effects of Stx2 is related to an immunophenotype change of tubular cells and the presence of mild fibrosis in the interstitium.Fil: Ochoa, Federico Claudio. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaFil: Lago, N. R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; ArgentinaFil: Gerhardt, Elizabeth. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaKarger2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198389Ochoa, Federico Claudio; Lago, N. R.; Gerhardt, Elizabeth; Ibarra, Cristina Adriana; Zotta, E.; Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome; Karger; American Journal Of Nephrology; 32; 4; 8-2010; 340-3460250-8095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://karger.com/ajn/article-abstract/32/4/340/26356/Characterization-of-Stx2-Tubular-Response-in-a-Rat?redirectedFrom=fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1159/000319444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:01Zoai:ri.conicet.gov.ar:11336/198389instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:01.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
title Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
spellingShingle Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
Ochoa, Federico Claudio
ACUTE RENAL FAILURE
ALPHA-SMOOTH MUSCLE ACTIN
EPITHELIAL MARKERS
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN
TRANSFORMING GROWTH FACTOR-BETA1
title_short Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
title_full Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
title_fullStr Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
title_full_unstemmed Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
title_sort Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome
dc.creator.none.fl_str_mv Ochoa, Federico Claudio
Lago, N. R.
Gerhardt, Elizabeth
Ibarra, Cristina Adriana
Zotta, E.
author Ochoa, Federico Claudio
author_facet Ochoa, Federico Claudio
Lago, N. R.
Gerhardt, Elizabeth
Ibarra, Cristina Adriana
Zotta, E.
author_role author
author2 Lago, N. R.
Gerhardt, Elizabeth
Ibarra, Cristina Adriana
Zotta, E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACUTE RENAL FAILURE
ALPHA-SMOOTH MUSCLE ACTIN
EPITHELIAL MARKERS
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN
TRANSFORMING GROWTH FACTOR-BETA1
topic ACUTE RENAL FAILURE
ALPHA-SMOOTH MUSCLE ACTIN
EPITHELIAL MARKERS
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN
TRANSFORMING GROWTH FACTOR-BETA1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In Argentina, hemolytic uremic syndrome (HUS) constitutes the most frequent cause of acute renal failure in children. The aim of our study was to analyze the early tubular response under the effect of Shiga toxin type 2 (Stx2) in a rat experimental model of HUS. METHODS: Adult male Sprague-Dawley rats were injected intraperitoneally with culture supernatant from recombinant Escherichia coli expressing Stx2. Functional, histological, immunohistochemical and Western blot studies were performed at 48 h postinoculation. RESULTS: Renal tubules showed the loss of the epithelial markers E-cadherin and beta-catenin, and an increase in transforming growth factor-beta1 expression. We detected the expression of alpha-smooth muscle actin in the interstitium and fibrosis in the periglomerular areas. CONCLUSION: Our results indicate that the early tubular response to the effects of Stx2 is related to an immunophenotype change of tubular cells and the presence of mild fibrosis in the interstitium.
Fil: Ochoa, Federico Claudio. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Lago, N. R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Centro de Patología Experimental; Argentina
Fil: Gerhardt, Elizabeth. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
description In Argentina, hemolytic uremic syndrome (HUS) constitutes the most frequent cause of acute renal failure in children. The aim of our study was to analyze the early tubular response under the effect of Shiga toxin type 2 (Stx2) in a rat experimental model of HUS. METHODS: Adult male Sprague-Dawley rats were injected intraperitoneally with culture supernatant from recombinant Escherichia coli expressing Stx2. Functional, histological, immunohistochemical and Western blot studies were performed at 48 h postinoculation. RESULTS: Renal tubules showed the loss of the epithelial markers E-cadherin and beta-catenin, and an increase in transforming growth factor-beta1 expression. We detected the expression of alpha-smooth muscle actin in the interstitium and fibrosis in the periglomerular areas. CONCLUSION: Our results indicate that the early tubular response to the effects of Stx2 is related to an immunophenotype change of tubular cells and the presence of mild fibrosis in the interstitium.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198389
Ochoa, Federico Claudio; Lago, N. R.; Gerhardt, Elizabeth; Ibarra, Cristina Adriana; Zotta, E.; Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome; Karger; American Journal Of Nephrology; 32; 4; 8-2010; 340-346
0250-8095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198389
identifier_str_mv Ochoa, Federico Claudio; Lago, N. R.; Gerhardt, Elizabeth; Ibarra, Cristina Adriana; Zotta, E.; Characterization of Stx2 tubular response in a rat experimental model of hemolytic uremic syndrome; Karger; American Journal Of Nephrology; 32; 4; 8-2010; 340-346
0250-8095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://karger.com/ajn/article-abstract/32/4/340/26356/Characterization-of-Stx2-Tubular-Response-in-a-Rat?redirectedFrom=fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1159/000319444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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