Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome

Autores
Fernández, Gabriela Cristina; Rubel, Carolina; Dran, Graciela Isabel; Gómez, Sonia Alejandra; Isturiz, Martín Amadeo; Palermo, Marina Sandra
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has been demonstrated that infections due to Shiga toxins (Stx) producing Escherichia coli are the main cause of the hemolytic uremic syndrome (HUS). Although it is recognized that Stx damage the glomerular endothelium, clinical and experimental evidence suggests that the inflammatory response is able to potentiate Stx toxicity. Lipopolysaccharides (LPS) and neutrophils (PMN) represent two central components of inflammation during a gram-negative infection. In this regard, patients with high peripheral PMN counts at presentation have a poor prognosis. Since the murine model has been used to study LPS-Stx interactions, we analyzed the effects of Stx alone or in combination with LPS on the kinetics of neutrophil production and activation and their participation in renal damage. We observed a sustained neutrophilia after Stx2 injection. Moreover, these neutrophils showed increased expression of CD11b, enhanced cytotoxic capacity, and greater adhesive properties. Regarding the cooperative effects of LPS on Stx2 action, we demonstrated potentiation of neutrophilia and CD11b induction at early times by pretreatment with LPS. Finally, a positive correlation between neutrophil percentage and renal damage (assayed as plasmatic urea) firmly suggests a role for PMN in the pathogenesis of HUS. (C) 2000 Academic Press.
Fil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rubel, Carolina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Hemolytic Uremic Syndrome
Neutrophils
Shiga Toxin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54810
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54810
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndromeFernández, Gabriela CristinaRubel, CarolinaDran, Graciela IsabelGómez, Sonia AlejandraIsturiz, Martín AmadeoPalermo, Marina SandraHemolytic Uremic SyndromeNeutrophilsShiga Toxinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has been demonstrated that infections due to Shiga toxins (Stx) producing Escherichia coli are the main cause of the hemolytic uremic syndrome (HUS). Although it is recognized that Stx damage the glomerular endothelium, clinical and experimental evidence suggests that the inflammatory response is able to potentiate Stx toxicity. Lipopolysaccharides (LPS) and neutrophils (PMN) represent two central components of inflammation during a gram-negative infection. In this regard, patients with high peripheral PMN counts at presentation have a poor prognosis. Since the murine model has been used to study LPS-Stx interactions, we analyzed the effects of Stx alone or in combination with LPS on the kinetics of neutrophil production and activation and their participation in renal damage. We observed a sustained neutrophilia after Stx2 injection. Moreover, these neutrophils showed increased expression of CD11b, enhanced cytotoxic capacity, and greater adhesive properties. Regarding the cooperative effects of LPS on Stx2 action, we demonstrated potentiation of neutrophilia and CD11b induction at early times by pretreatment with LPS. Finally, a positive correlation between neutrophil percentage and renal damage (assayed as plasmatic urea) firmly suggests a role for PMN in the pathogenesis of HUS. (C) 2000 Academic Press.Fil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Rubel, Carolina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaAcademic Press Inc Elsevier Science2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54810Fernández, Gabriela Cristina; Rubel, Carolina; Dran, Graciela Isabel; Gómez, Sonia Alejandra; Isturiz, Martín Amadeo; et al.; Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome; Academic Press Inc Elsevier Science; Clinical Immunology; 95; 3; 12-2000; 227-2341521-6616CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/clim.2000.4862info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1521661600948629info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:51Zoai:ri.conicet.gov.ar:11336/54810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:52.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
title Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
spellingShingle Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
Fernández, Gabriela Cristina
Hemolytic Uremic Syndrome
Neutrophils
Shiga Toxin
title_short Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
title_full Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
title_fullStr Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
title_full_unstemmed Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
title_sort Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome
dc.creator.none.fl_str_mv Fernández, Gabriela Cristina
Rubel, Carolina
Dran, Graciela Isabel
Gómez, Sonia Alejandra
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author Fernández, Gabriela Cristina
author_facet Fernández, Gabriela Cristina
Rubel, Carolina
Dran, Graciela Isabel
Gómez, Sonia Alejandra
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author_role author
author2 Rubel, Carolina
Dran, Graciela Isabel
Gómez, Sonia Alejandra
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hemolytic Uremic Syndrome
Neutrophils
Shiga Toxin
topic Hemolytic Uremic Syndrome
Neutrophils
Shiga Toxin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It has been demonstrated that infections due to Shiga toxins (Stx) producing Escherichia coli are the main cause of the hemolytic uremic syndrome (HUS). Although it is recognized that Stx damage the glomerular endothelium, clinical and experimental evidence suggests that the inflammatory response is able to potentiate Stx toxicity. Lipopolysaccharides (LPS) and neutrophils (PMN) represent two central components of inflammation during a gram-negative infection. In this regard, patients with high peripheral PMN counts at presentation have a poor prognosis. Since the murine model has been used to study LPS-Stx interactions, we analyzed the effects of Stx alone or in combination with LPS on the kinetics of neutrophil production and activation and their participation in renal damage. We observed a sustained neutrophilia after Stx2 injection. Moreover, these neutrophils showed increased expression of CD11b, enhanced cytotoxic capacity, and greater adhesive properties. Regarding the cooperative effects of LPS on Stx2 action, we demonstrated potentiation of neutrophilia and CD11b induction at early times by pretreatment with LPS. Finally, a positive correlation between neutrophil percentage and renal damage (assayed as plasmatic urea) firmly suggests a role for PMN in the pathogenesis of HUS. (C) 2000 Academic Press.
Fil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Rubel, Carolina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
description It has been demonstrated that infections due to Shiga toxins (Stx) producing Escherichia coli are the main cause of the hemolytic uremic syndrome (HUS). Although it is recognized that Stx damage the glomerular endothelium, clinical and experimental evidence suggests that the inflammatory response is able to potentiate Stx toxicity. Lipopolysaccharides (LPS) and neutrophils (PMN) represent two central components of inflammation during a gram-negative infection. In this regard, patients with high peripheral PMN counts at presentation have a poor prognosis. Since the murine model has been used to study LPS-Stx interactions, we analyzed the effects of Stx alone or in combination with LPS on the kinetics of neutrophil production and activation and their participation in renal damage. We observed a sustained neutrophilia after Stx2 injection. Moreover, these neutrophils showed increased expression of CD11b, enhanced cytotoxic capacity, and greater adhesive properties. Regarding the cooperative effects of LPS on Stx2 action, we demonstrated potentiation of neutrophilia and CD11b induction at early times by pretreatment with LPS. Finally, a positive correlation between neutrophil percentage and renal damage (assayed as plasmatic urea) firmly suggests a role for PMN in the pathogenesis of HUS. (C) 2000 Academic Press.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54810
Fernández, Gabriela Cristina; Rubel, Carolina; Dran, Graciela Isabel; Gómez, Sonia Alejandra; Isturiz, Martín Amadeo; et al.; Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome; Academic Press Inc Elsevier Science; Clinical Immunology; 95; 3; 12-2000; 227-234
1521-6616
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54810
identifier_str_mv Fernández, Gabriela Cristina; Rubel, Carolina; Dran, Graciela Isabel; Gómez, Sonia Alejandra; Isturiz, Martín Amadeo; et al.; Shiga toxin-2 induces neutrophilia and neutrophil activation in a murine model of hemolytic uremic syndrome; Academic Press Inc Elsevier Science; Clinical Immunology; 95; 3; 12-2000; 227-234
1521-6616
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/clim.2000.4862
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1521661600948629
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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