Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides

Autores
Ugalde, Rodolfo A.; Staneloni, Roberto Julio; Leloir, Luis Federico
Año de publicación
1980
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal of glucose from the glucose-containing oligosaccharide. One acts on the oligosaccharide containing three glucose residues and another on the oligosaccharide which has one or two glucoses. The glucosidase which acts on (Glc)2(Man)9(GlcNAc)2 could be purified with a Concanavalin-A--Sepharose column following by electrofocusing. This purified preparation was active on the oligosaccharide containing one or two glucoses. Heat inactivation and inhibition by disaccharides was parallel for both activities. Inhibition of the glucosidase active on (Glc)3(Man)9(GlcNAC)2 was obtained with kojibiose which has an alpha 1-2 linkage, while the glucosidase acting on (Glc)1-2(Man)9-(GlcNAc)2 was inhibited by nigerose (alpha 1-3 linkage), maltose (alpha 1-4 linkage) and glucose at a higher concentration. None of the beta anomers inhibited. These results are consistent with an alpha configuration of the three glucoses of the dolichyl-diphosphate-linked oligosaccharide. Kojibiose was found to inhibit glucosidase action not only on the free oligosaccharide but also on protein-bound one.
original
Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Staneloni, Roberto Julio. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Escala de grises
7 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
Materia
Leloir, Luis F.
Ugalde, Rodolfo A.
Staneloni, Roberto J.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143170
Acceder
id CONICETDig_6fb38d103de6773c1ac3b7d9ab81e667
oai_identifier_str oai:ri.conicet.gov.ar:11336/143170
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharidesUgalde, Rodolfo A.Staneloni, Roberto JulioLeloir, Luis FedericoLeloir, Luis F.Ugalde, Rodolfo A.Staneloni, Roberto J.Leloir InvestigadorPublicacionesLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto LeloirArtículos Científicoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal of glucose from the glucose-containing oligosaccharide. One acts on the oligosaccharide containing three glucose residues and another on the oligosaccharide which has one or two glucoses. The glucosidase which acts on (Glc)2(Man)9(GlcNAc)2 could be purified with a Concanavalin-A--Sepharose column following by electrofocusing. This purified preparation was active on the oligosaccharide containing one or two glucoses. Heat inactivation and inhibition by disaccharides was parallel for both activities. Inhibition of the glucosidase active on (Glc)3(Man)9(GlcNAC)2 was obtained with kojibiose which has an alpha 1-2 linkage, while the glucosidase acting on (Glc)1-2(Man)9-(GlcNAc)2 was inhibited by nigerose (alpha 1-3 linkage), maltose (alpha 1-4 linkage) and glucose at a higher concentration. None of the beta anomers inhibited. These results are consistent with an alpha configuration of the three glucoses of the dolichyl-diphosphate-linked oligosaccharide. Kojibiose was found to inhibit glucosidase action not only on the free oligosaccharide but also on protein-bound one.originalFil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Staneloni, Roberto Julio. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaEscala de grises7 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018Wiley1980-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaEscalaGrisartículoNoapplication/pdfhttp://hdl.handle.net/11336/143170Ugalde, Rodolfo A.; Staneloni, Roberto Julio; Leloir, Luis Federico; Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides; Wiley; European Journal Of Biochemistry; 113; 1; 12-1980; 97-1030014-2956enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1432-1033.1980.tb06144.xinfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/7460954/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.197info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:07Zoai:ri.conicet.gov.ar:11336/143170instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:07.433CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
title Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
spellingShingle Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
Ugalde, Rodolfo A.
Leloir, Luis F.
Ugalde, Rodolfo A.
Staneloni, Roberto J.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
title_short Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
title_full Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
title_fullStr Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
title_full_unstemmed Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
title_sort Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides
dc.creator.none.fl_str_mv Ugalde, Rodolfo A.
Staneloni, Roberto Julio
Leloir, Luis Federico
author Ugalde, Rodolfo A.
author_facet Ugalde, Rodolfo A.
Staneloni, Roberto Julio
Leloir, Luis Federico
author_role author
author2 Staneloni, Roberto Julio
Leloir, Luis Federico
author2_role author
author
dc.subject.none.fl_str_mv Leloir, Luis F.
Ugalde, Rodolfo A.
Staneloni, Roberto J.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
topic Leloir, Luis F.
Ugalde, Rodolfo A.
Staneloni, Roberto J.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal of glucose from the glucose-containing oligosaccharide. One acts on the oligosaccharide containing three glucose residues and another on the oligosaccharide which has one or two glucoses. The glucosidase which acts on (Glc)2(Man)9(GlcNAc)2 could be purified with a Concanavalin-A--Sepharose column following by electrofocusing. This purified preparation was active on the oligosaccharide containing one or two glucoses. Heat inactivation and inhibition by disaccharides was parallel for both activities. Inhibition of the glucosidase active on (Glc)3(Man)9(GlcNAC)2 was obtained with kojibiose which has an alpha 1-2 linkage, while the glucosidase acting on (Glc)1-2(Man)9-(GlcNAc)2 was inhibited by nigerose (alpha 1-3 linkage), maltose (alpha 1-4 linkage) and glucose at a higher concentration. None of the beta anomers inhibited. These results are consistent with an alpha configuration of the three glucoses of the dolichyl-diphosphate-linked oligosaccharide. Kojibiose was found to inhibit glucosidase action not only on the free oligosaccharide but also on protein-bound one.
original
Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Staneloni, Roberto Julio. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Escala de grises
7 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
description Further work on microsomal glucosidases of rat liver has confirmed that at least two enzymes are involved in the removal of glucose from the glucose-containing oligosaccharide. One acts on the oligosaccharide containing three glucose residues and another on the oligosaccharide which has one or two glucoses. The glucosidase which acts on (Glc)2(Man)9(GlcNAc)2 could be purified with a Concanavalin-A--Sepharose column following by electrofocusing. This purified preparation was active on the oligosaccharide containing one or two glucoses. Heat inactivation and inhibition by disaccharides was parallel for both activities. Inhibition of the glucosidase active on (Glc)3(Man)9(GlcNAC)2 was obtained with kojibiose which has an alpha 1-2 linkage, while the glucosidase acting on (Glc)1-2(Man)9-(GlcNAc)2 was inhibited by nigerose (alpha 1-3 linkage), maltose (alpha 1-4 linkage) and glucose at a higher concentration. None of the beta anomers inhibited. These results are consistent with an alpha configuration of the three glucoses of the dolichyl-diphosphate-linked oligosaccharide. Kojibiose was found to inhibit glucosidase action not only on the free oligosaccharide but also on protein-bound one.
publishDate 1980
dc.date.none.fl_str_mv 1980-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143170
Ugalde, Rodolfo A.; Staneloni, Roberto Julio; Leloir, Luis Federico; Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides; Wiley; European Journal Of Biochemistry; 113; 1; 12-1980; 97-103
0014-2956
url http://hdl.handle.net/11336/143170
identifier_str_mv Ugalde, Rodolfo A.; Staneloni, Roberto Julio; Leloir, Luis Federico; Microsomal glucosidases of rat liver. Partial purification and inhibition by disaccharides; Wiley; European Journal Of Biochemistry; 113; 1; 12-1980; 97-103
0014-2956
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1432-1033.1980.tb06144.x
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/7460954/
Ciudad de Buenos Aires
Fundación Instituto Leloir
Consejo Nacional de Investigaciones Científicas y Técnicas
Proyecto Houssay y Leloir
Leloir, Luis F.
LFL-PI-O-ART.197
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
Derivada
EscalaGris
artículo
No
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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