An intermediate in cyclic beta 1-2 glucan biosynthesis

Autores
Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico
Año de publicación
1985
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.
original
Fil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Escala de grises
6 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
Materia
Leloir, Luis F.
Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143171
Acceder
id CONICETDig_9353bdd3cb282fcfa35abd5bad8b821e
oai_identifier_str oai:ri.conicet.gov.ar:11336/143171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An intermediate in cyclic beta 1-2 glucan biosynthesisZorreguieta, ÁngelesUgalde, Rodolfo A.Leloir, Luis FedericoLeloir, Luis F.Zorreguieta, ÁngelesUgalde, Rodolfo A.Leloir InvestigadorPublicacionesLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto LeloirArtículos Científicoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.originalFil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaEscala de grises6 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018Academic Press1985-01-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaEscalaGrisartículoNoapplication/pdfhttp://hdl.handle.net/11336/143171Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-3570006-291Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/0006-291X(85)90613-8info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/3970697/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:46Zoai:ri.conicet.gov.ar:11336/143171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:46.388CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An intermediate in cyclic beta 1-2 glucan biosynthesis
title An intermediate in cyclic beta 1-2 glucan biosynthesis
spellingShingle An intermediate in cyclic beta 1-2 glucan biosynthesis
Zorreguieta, Ángeles
Leloir, Luis F.
Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
title_short An intermediate in cyclic beta 1-2 glucan biosynthesis
title_full An intermediate in cyclic beta 1-2 glucan biosynthesis
title_fullStr An intermediate in cyclic beta 1-2 glucan biosynthesis
title_full_unstemmed An intermediate in cyclic beta 1-2 glucan biosynthesis
title_sort An intermediate in cyclic beta 1-2 glucan biosynthesis
dc.creator.none.fl_str_mv Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir, Luis Federico
author Zorreguieta, Ángeles
author_facet Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir, Luis Federico
author_role author
author2 Ugalde, Rodolfo A.
Leloir, Luis Federico
author2_role author
author
dc.subject.none.fl_str_mv Leloir, Luis F.
Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
topic Leloir, Luis F.
Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.
original
Fil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Escala de grises
6 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
description Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.
publishDate 1985
dc.date.none.fl_str_mv 1985-01-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143171
Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-357
0006-291X
url http://hdl.handle.net/11336/143171
identifier_str_mv Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-357
0006-291X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/0006-291X(85)90613-8
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/3970697/
Ciudad de Buenos Aires
Fundación Instituto Leloir
Consejo Nacional de Investigaciones Científicas y Técnicas
Proyecto Houssay y Leloir
Leloir, Luis F.
LFL-PI-O-ART.212
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
Derivada
EscalaGris
artículo
No
application/pdf
dc.publisher.none.fl_str_mv Academic Press
publisher.none.fl_str_mv Academic Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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