An intermediate in cyclic beta 1-2 glucan biosynthesis
- Autores
- Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico
- Año de publicación
- 1985
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.
original
Fil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Escala de grises
6 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018 - Materia
-
Leloir, Luis F.
Zorreguieta, Ángeles
Ugalde, Rodolfo A.
Leloir Investigador
Publicaciones
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Artículos Científicos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/143171
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/143171 |
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network_name_str |
CONICET Digital (CONICET) |
spelling |
An intermediate in cyclic beta 1-2 glucan biosynthesisZorreguieta, ÁngelesUgalde, Rodolfo A.Leloir, Luis FedericoLeloir, Luis F.Zorreguieta, ÁngelesUgalde, Rodolfo A.Leloir InvestigadorPublicacionesLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto LeloirArtículos Científicoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan.originalFil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaEscala de grises6 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018Academic Press1985-01-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaEscalaGrisartículoNoapplication/pdfhttp://hdl.handle.net/11336/143171Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-3570006-291Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/0006-291X(85)90613-8info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/3970697/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:25Zoai:ri.conicet.gov.ar:11336/143171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:25.901CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
title |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
spellingShingle |
An intermediate in cyclic beta 1-2 glucan biosynthesis Zorreguieta, Ángeles Leloir, Luis F. Zorreguieta, Ángeles Ugalde, Rodolfo A. Leloir Investigador Publicaciones Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir Artículos Científicos |
title_short |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
title_full |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
title_fullStr |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
title_full_unstemmed |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
title_sort |
An intermediate in cyclic beta 1-2 glucan biosynthesis |
dc.creator.none.fl_str_mv |
Zorreguieta, Ángeles Ugalde, Rodolfo A. Leloir, Luis Federico |
author |
Zorreguieta, Ángeles |
author_facet |
Zorreguieta, Ángeles Ugalde, Rodolfo A. Leloir, Luis Federico |
author_role |
author |
author2 |
Ugalde, Rodolfo A. Leloir, Luis Federico |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Leloir, Luis F. Zorreguieta, Ángeles Ugalde, Rodolfo A. Leloir Investigador Publicaciones Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir Artículos Científicos |
topic |
Leloir, Luis F. Zorreguieta, Ángeles Ugalde, Rodolfo A. Leloir Investigador Publicaciones Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir Artículos Científicos |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan. original Fil: Zorreguieta, Ángeles. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Ugalde, Rodolfo A.. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Escala de grises 6 páginas en pdf LFL-PI-O-ART. Artículos científicos Unidad documental simple AR-HYL-2018 |
description |
Incubation of UDP-[14C]Glc with the inner membranes of Agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. The proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. The cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. Addition of excess non-radioactive UDP-Glc to the reaction mixture nearly stops the formation of radioactive beta 1-2 glucan and leads to a rapid fall of radioactivity in the trichloroacetic acid precipitate. Alkaline treatment of the insoluble compounds under conditions of beta-elimination leads to the partial release of free saccharides (about 30%). It is concluded that beta 1-2 glucan chains are built up joined to a protein and then released as free cyclic beta 1-2 glucan. |
publishDate |
1985 |
dc.date.none.fl_str_mv |
1985-01-16 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/143171 Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-357 0006-291X |
url |
http://hdl.handle.net/11336/143171 |
identifier_str_mv |
Zorreguieta, Ángeles; Ugalde, Rodolfo A.; Leloir, Luis Federico; An intermediate in cyclic beta 1-2 glucan biosynthesis; Academic Press; Biochemical and Biophysical Research Communications; 126; 1; 16-1-1985; 352-357 0006-291X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/0006-291X(85)90613-8 info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/3970697/ Ciudad de Buenos Aires Fundación Instituto Leloir Consejo Nacional de Investigaciones Científicas y Técnicas Proyecto Houssay y Leloir Leloir, Luis F. LFL-PI-O-ART.212 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf Derivada EscalaGris artículo No application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press |
publisher.none.fl_str_mv |
Academic Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614385058709504 |
score |
13.070432 |