Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production

Autores
Palazzini, Juan Manuel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bacillus velezensis RC218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum sensu stricto, the causal agent of Fusarium head blight (FHB) in Argentina. We demonstrated the ability of B. velezensis RC218 to reduce disease severity and DON accumulation in vitro, under greenhouse and field trials. The current presentation extends characterizing B. velezensis RC218 by genome sequencing and secondary metabolite production. Secondary metabolite clusters wereidentified with antiSMASH3.0 or direct blasting. Culture supernatants containing secondary metabolites were analyzed by LC-MS. The field study demonstrated that B. velezensis RC 218 could reduce FHB severity and the associated mycotoxin (deoxynivalenol) production to undetectable levels. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the B. velezensis clade. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibioticericin, which is unique to this strain. This represents the first confirmed production of ericin by a B. velezensis strain. Biocontrol activity can be related to the production of several lipopeptides, from the surfactin, fengycin and iturin families. Microbiome analysis also demonstrated that application of the bioicontrol on spikes does not impactnative populations dynamics.
Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
First International “Microbes For Life” Webinar
Argel
Argelia
Ecole Normale Supérieur de Kouba. Laboratoire de Biologie des Systèmes Microbiens
Materia
Fusarium Head Blight
Bacillus velezensis
Metabolite profile
Biocontrol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232239
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile productionPalazzini, Juan ManuelFusarium Head BlightBacillus velezensisMetabolite profileBiocontrolhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus velezensis RC218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum sensu stricto, the causal agent of Fusarium head blight (FHB) in Argentina. We demonstrated the ability of B. velezensis RC218 to reduce disease severity and DON accumulation in vitro, under greenhouse and field trials. The current presentation extends characterizing B. velezensis RC218 by genome sequencing and secondary metabolite production. Secondary metabolite clusters wereidentified with antiSMASH3.0 or direct blasting. Culture supernatants containing secondary metabolites were analyzed by LC-MS. The field study demonstrated that B. velezensis RC 218 could reduce FHB severity and the associated mycotoxin (deoxynivalenol) production to undetectable levels. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the B. velezensis clade. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibioticericin, which is unique to this strain. This represents the first confirmed production of ericin by a B. velezensis strain. Biocontrol activity can be related to the production of several lipopeptides, from the surfactin, fengycin and iturin families. Microbiome analysis also demonstrated that application of the bioicontrol on spikes does not impactnative populations dynamics.Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFirst International “Microbes For Life” WebinarArgelArgeliaEcole Normale Supérieur de Kouba. Laboratoire de Biologie des Systèmes MicrobiensUniversity Algeria2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWebinarBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232239Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production; First International “Microbes For Life” Webinar; Argel ; Argelia; 2021; 32-32CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://lbsm-lab.wixsite.com/microbesforlife2021Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:52Zoai:ri.conicet.gov.ar:11336/232239instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:52.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
title Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
spellingShingle Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
Palazzini, Juan Manuel
Fusarium Head Blight
Bacillus velezensis
Metabolite profile
Biocontrol
title_short Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
title_full Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
title_fullStr Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
title_full_unstemmed Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
title_sort Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production
dc.creator.none.fl_str_mv Palazzini, Juan Manuel
author Palazzini, Juan Manuel
author_facet Palazzini, Juan Manuel
author_role author
dc.subject.none.fl_str_mv Fusarium Head Blight
Bacillus velezensis
Metabolite profile
Biocontrol
topic Fusarium Head Blight
Bacillus velezensis
Metabolite profile
Biocontrol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus velezensis RC218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum sensu stricto, the causal agent of Fusarium head blight (FHB) in Argentina. We demonstrated the ability of B. velezensis RC218 to reduce disease severity and DON accumulation in vitro, under greenhouse and field trials. The current presentation extends characterizing B. velezensis RC218 by genome sequencing and secondary metabolite production. Secondary metabolite clusters wereidentified with antiSMASH3.0 or direct blasting. Culture supernatants containing secondary metabolites were analyzed by LC-MS. The field study demonstrated that B. velezensis RC 218 could reduce FHB severity and the associated mycotoxin (deoxynivalenol) production to undetectable levels. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the B. velezensis clade. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibioticericin, which is unique to this strain. This represents the first confirmed production of ericin by a B. velezensis strain. Biocontrol activity can be related to the production of several lipopeptides, from the surfactin, fengycin and iturin families. Microbiome analysis also demonstrated that application of the bioicontrol on spikes does not impactnative populations dynamics.
Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
First International “Microbes For Life” Webinar
Argel
Argelia
Ecole Normale Supérieur de Kouba. Laboratoire de Biologie des Systèmes Microbiens
description Bacillus velezensis RC218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum sensu stricto, the causal agent of Fusarium head blight (FHB) in Argentina. We demonstrated the ability of B. velezensis RC218 to reduce disease severity and DON accumulation in vitro, under greenhouse and field trials. The current presentation extends characterizing B. velezensis RC218 by genome sequencing and secondary metabolite production. Secondary metabolite clusters wereidentified with antiSMASH3.0 or direct blasting. Culture supernatants containing secondary metabolites were analyzed by LC-MS. The field study demonstrated that B. velezensis RC 218 could reduce FHB severity and the associated mycotoxin (deoxynivalenol) production to undetectable levels. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the B. velezensis clade. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibioticericin, which is unique to this strain. This represents the first confirmed production of ericin by a B. velezensis strain. Biocontrol activity can be related to the production of several lipopeptides, from the surfactin, fengycin and iturin families. Microbiome analysis also demonstrated that application of the bioicontrol on spikes does not impactnative populations dynamics.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Webinar
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232239
Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production; First International “Microbes For Life” Webinar; Argel ; Argelia; 2021; 32-32
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232239
identifier_str_mv Bacillus velezensis Rc218 as a biocontrol agent against Fusarium head blight of wheat: revealing genome insights and metabolite profile production; First International “Microbes For Life” Webinar; Argel ; Argelia; 2021; 32-32
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://lbsm-lab.wixsite.com/microbesforlife2021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv University Algeria
publisher.none.fl_str_mv University Algeria
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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