Biofabrication of functional protein nanoparticles through simple His-tag engineering
- Autores
- López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; Parladé, Eloi; Sánchez Chardi, Alejandro; Voltà Durán, Eric; Serna, Naroa; Cano Garrido, Olivia; Flores Mamani, Sandra Soledad; Ferrer Miralles, Neus; Nolan, María Verónica; de Marco, Ario; Roher, Nerea; Unzueta, Ugutz; Vazquez, Esther; Villaverde, Antonio
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.
Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; España
Fil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; España
Fil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; España
Fil: Sánchez García, Laura. Universitat Autònoma de Barcelona; España
Fil: Parladé, Eloi. Universitat Autònoma de Barcelona; España
Fil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; España
Fil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; España
Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España
Fil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; España
Fil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; España
Fil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: de Marco, Ario. University of Nova Gorica; Eslovenia
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España
Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España
Fil: Villaverde, Antonio. Universitat Autònoma de Barcelona; España - Materia
-
NANOPARTICLES
PROTEIN ENGINEERING
DIVALENT CATIONS
PROTEIN MATERIALS
BIOMATERIALS DESIGN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158814
Ver los metadatos del registro completo
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Biofabrication of functional protein nanoparticles through simple His-tag engineeringLópez Laguna, HèctorSanchez, Julieta MariaCarratalá, José VicenteRojas Peña, MauricioSánchez García, LauraParladé, EloiSánchez Chardi, AlejandroVoltà Durán, EricSerna, NaroaCano Garrido, OliviaFlores Mamani, Sandra SoledadFerrer Miralles, NeusNolan, María Verónicade Marco, ArioRoher, NereaUnzueta, UgutzVazquez, EstherVillaverde, AntonioNANOPARTICLESPROTEIN ENGINEERINGDIVALENT CATIONSPROTEIN MATERIALSBIOMATERIALS DESIGNhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; EspañaFil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; EspañaFil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; EspañaFil: Sánchez García, Laura. Universitat Autònoma de Barcelona; EspañaFil: Parladé, Eloi. Universitat Autònoma de Barcelona; EspañaFil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; EspañaFil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; EspañaFil: Serna, Naroa. Universitat Autònoma de Barcelona; EspañaFil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; EspañaFil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; EspañaFil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: de Marco, Ario. University of Nova Gorica; EsloveniaFil: Roher, Nerea. Universitat Autònoma de Barcelona; EspañaFil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; EspañaFil: Vazquez, Esther. Universitat Autònoma de Barcelona; EspañaFil: Villaverde, Antonio. Universitat Autònoma de Barcelona; EspañaAmerican Chemical Society Inc2021-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158814López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-123542168-0485CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acssuschemeng.1c04256info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssuschemeng.1c04256info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:31Zoai:ri.conicet.gov.ar:11336/158814instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:31.284CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
title |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
spellingShingle |
Biofabrication of functional protein nanoparticles through simple His-tag engineering López Laguna, Hèctor NANOPARTICLES PROTEIN ENGINEERING DIVALENT CATIONS PROTEIN MATERIALS BIOMATERIALS DESIGN |
title_short |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
title_full |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
title_fullStr |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
title_full_unstemmed |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
title_sort |
Biofabrication of functional protein nanoparticles through simple His-tag engineering |
dc.creator.none.fl_str_mv |
López Laguna, Hèctor Sanchez, Julieta Maria Carratalá, José Vicente Rojas Peña, Mauricio Sánchez García, Laura Parladé, Eloi Sánchez Chardi, Alejandro Voltà Durán, Eric Serna, Naroa Cano Garrido, Olivia Flores Mamani, Sandra Soledad Ferrer Miralles, Neus Nolan, María Verónica de Marco, Ario Roher, Nerea Unzueta, Ugutz Vazquez, Esther Villaverde, Antonio |
author |
López Laguna, Hèctor |
author_facet |
López Laguna, Hèctor Sanchez, Julieta Maria Carratalá, José Vicente Rojas Peña, Mauricio Sánchez García, Laura Parladé, Eloi Sánchez Chardi, Alejandro Voltà Durán, Eric Serna, Naroa Cano Garrido, Olivia Flores Mamani, Sandra Soledad Ferrer Miralles, Neus Nolan, María Verónica de Marco, Ario Roher, Nerea Unzueta, Ugutz Vazquez, Esther Villaverde, Antonio |
author_role |
author |
author2 |
Sanchez, Julieta Maria Carratalá, José Vicente Rojas Peña, Mauricio Sánchez García, Laura Parladé, Eloi Sánchez Chardi, Alejandro Voltà Durán, Eric Serna, Naroa Cano Garrido, Olivia Flores Mamani, Sandra Soledad Ferrer Miralles, Neus Nolan, María Verónica de Marco, Ario Roher, Nerea Unzueta, Ugutz Vazquez, Esther Villaverde, Antonio |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
NANOPARTICLES PROTEIN ENGINEERING DIVALENT CATIONS PROTEIN MATERIALS BIOMATERIALS DESIGN |
topic |
NANOPARTICLES PROTEIN ENGINEERING DIVALENT CATIONS PROTEIN MATERIALS BIOMATERIALS DESIGN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats. Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; España Fil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; España Fil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; España Fil: Sánchez García, Laura. Universitat Autònoma de Barcelona; España Fil: Parladé, Eloi. Universitat Autònoma de Barcelona; España Fil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; España Fil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; España Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España Fil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; España Fil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; España Fil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: de Marco, Ario. University of Nova Gorica; Eslovenia Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España Fil: Villaverde, Antonio. Universitat Autònoma de Barcelona; España |
description |
We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-30 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/158814 López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-12354 2168-0485 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158814 |
identifier_str_mv |
López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-12354 2168-0485 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1021/acssuschemeng.1c04256 info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssuschemeng.1c04256 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society Inc |
publisher.none.fl_str_mv |
American Chemical Society Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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score |
13.070432 |