Biofabrication of functional protein nanoparticles through simple His-tag engineering

Autores
López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; Parladé, Eloi; Sánchez Chardi, Alejandro; Voltà Durán, Eric; Serna, Naroa; Cano Garrido, Olivia; Flores Mamani, Sandra Soledad; Ferrer Miralles, Neus; Nolan, María Verónica; de Marco, Ario; Roher, Nerea; Unzueta, Ugutz; Vazquez, Esther; Villaverde, Antonio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.
Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; España
Fil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; España
Fil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; España
Fil: Sánchez García, Laura. Universitat Autònoma de Barcelona; España
Fil: Parladé, Eloi. Universitat Autònoma de Barcelona; España
Fil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; España
Fil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; España
Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España
Fil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; España
Fil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; España
Fil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: de Marco, Ario. University of Nova Gorica; Eslovenia
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España
Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España
Fil: Villaverde, Antonio. Universitat Autònoma de Barcelona; España
Materia
NANOPARTICLES
PROTEIN ENGINEERING
DIVALENT CATIONS
PROTEIN MATERIALS
BIOMATERIALS DESIGN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158814
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/158814
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biofabrication of functional protein nanoparticles through simple His-tag engineeringLópez Laguna, HèctorSanchez, Julieta MariaCarratalá, José VicenteRojas Peña, MauricioSánchez García, LauraParladé, EloiSánchez Chardi, AlejandroVoltà Durán, EricSerna, NaroaCano Garrido, OliviaFlores Mamani, Sandra SoledadFerrer Miralles, NeusNolan, María Verónicade Marco, ArioRoher, NereaUnzueta, UgutzVazquez, EstherVillaverde, AntonioNANOPARTICLESPROTEIN ENGINEERINGDIVALENT CATIONSPROTEIN MATERIALSBIOMATERIALS DESIGNhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; EspañaFil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; EspañaFil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; EspañaFil: Sánchez García, Laura. Universitat Autònoma de Barcelona; EspañaFil: Parladé, Eloi. Universitat Autònoma de Barcelona; EspañaFil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; EspañaFil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; EspañaFil: Serna, Naroa. Universitat Autònoma de Barcelona; EspañaFil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; EspañaFil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; EspañaFil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: de Marco, Ario. University of Nova Gorica; EsloveniaFil: Roher, Nerea. Universitat Autònoma de Barcelona; EspañaFil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; EspañaFil: Vazquez, Esther. Universitat Autònoma de Barcelona; EspañaFil: Villaverde, Antonio. Universitat Autònoma de Barcelona; EspañaAmerican Chemical Society Inc2021-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158814López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-123542168-0485CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acssuschemeng.1c04256info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssuschemeng.1c04256info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:26Zoai:ri.conicet.gov.ar:11336/158814instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:27.173CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofabrication of functional protein nanoparticles through simple His-tag engineering
title Biofabrication of functional protein nanoparticles through simple His-tag engineering
spellingShingle Biofabrication of functional protein nanoparticles through simple His-tag engineering
López Laguna, Hèctor
NANOPARTICLES
PROTEIN ENGINEERING
DIVALENT CATIONS
PROTEIN MATERIALS
BIOMATERIALS DESIGN
title_short Biofabrication of functional protein nanoparticles through simple His-tag engineering
title_full Biofabrication of functional protein nanoparticles through simple His-tag engineering
title_fullStr Biofabrication of functional protein nanoparticles through simple His-tag engineering
title_full_unstemmed Biofabrication of functional protein nanoparticles through simple His-tag engineering
title_sort Biofabrication of functional protein nanoparticles through simple His-tag engineering
dc.creator.none.fl_str_mv López Laguna, Hèctor
Sanchez, Julieta Maria
Carratalá, José Vicente
Rojas Peña, Mauricio
Sánchez García, Laura
Parladé, Eloi
Sánchez Chardi, Alejandro
Voltà Durán, Eric
Serna, Naroa
Cano Garrido, Olivia
Flores Mamani, Sandra Soledad
Ferrer Miralles, Neus
Nolan, María Verónica
de Marco, Ario
Roher, Nerea
Unzueta, Ugutz
Vazquez, Esther
Villaverde, Antonio
author López Laguna, Hèctor
author_facet López Laguna, Hèctor
Sanchez, Julieta Maria
Carratalá, José Vicente
Rojas Peña, Mauricio
Sánchez García, Laura
Parladé, Eloi
Sánchez Chardi, Alejandro
Voltà Durán, Eric
Serna, Naroa
Cano Garrido, Olivia
Flores Mamani, Sandra Soledad
Ferrer Miralles, Neus
Nolan, María Verónica
de Marco, Ario
Roher, Nerea
Unzueta, Ugutz
Vazquez, Esther
Villaverde, Antonio
author_role author
author2 Sanchez, Julieta Maria
Carratalá, José Vicente
Rojas Peña, Mauricio
Sánchez García, Laura
Parladé, Eloi
Sánchez Chardi, Alejandro
Voltà Durán, Eric
Serna, Naroa
Cano Garrido, Olivia
Flores Mamani, Sandra Soledad
Ferrer Miralles, Neus
Nolan, María Verónica
de Marco, Ario
Roher, Nerea
Unzueta, Ugutz
Vazquez, Esther
Villaverde, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOPARTICLES
PROTEIN ENGINEERING
DIVALENT CATIONS
PROTEIN MATERIALS
BIOMATERIALS DESIGN
topic NANOPARTICLES
PROTEIN ENGINEERING
DIVALENT CATIONS
PROTEIN MATERIALS
BIOMATERIALS DESIGN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.
Fil: López Laguna, Hèctor. Universitat Autònoma de Barcelona; España
Fil: Sanchez, Julieta Maria. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Carratalá, José Vicente. Universitat Autònoma de Barcelona; España
Fil: Rojas Peña, Mauricio. Universitat Autònoma de Barcelona; España
Fil: Sánchez García, Laura. Universitat Autònoma de Barcelona; España
Fil: Parladé, Eloi. Universitat Autònoma de Barcelona; España
Fil: Sánchez Chardi, Alejandro. Universitat Autònoma de Barcelona; España
Fil: Voltà Durán, Eric. Universitat Autònoma de Barcelona; España
Fil: Serna, Naroa. Universitat Autònoma de Barcelona; España
Fil: Cano Garrido, Olivia. Universitat Autònoma de Barcelona; España
Fil: Flores Mamani, Sandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ferrer Miralles, Neus. Universitat Autònoma de Barcelona; España
Fil: Nolan, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: de Marco, Ario. University of Nova Gorica; Eslovenia
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Unzueta, Ugutz. Universitat Autònoma de Barcelona; España
Fil: Vazquez, Esther. Universitat Autònoma de Barcelona; España
Fil: Villaverde, Antonio. Universitat Autònoma de Barcelona; España
description We have developed a simple, robust and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in the spontaneous and reproducible protein assembling as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembling of these materials is not linked to particular polypeptide features and it is based on an environment-friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, architecturally stable, fully functional, and serve as intermediates in a more complex assembling process resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforward applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158814
López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-12354
2168-0485
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158814
identifier_str_mv López Laguna, Hèctor; Sanchez, Julieta Maria; Carratalá, José Vicente; Rojas Peña, Mauricio; Sánchez García, Laura; et al.; Biofabrication of functional protein nanoparticles through simple His-tag engineering; American Chemical Society Inc; ACS Sustainable Chemistry and Engineering; 9; 36; 30-8-2021; 12341-12354
2168-0485
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/acssuschemeng.1c04256
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssuschemeng.1c04256
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society Inc
publisher.none.fl_str_mv American Chemical Society Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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