Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas

Autores
Imperato, Valeria; Portillo Estrada, Miguel; Saran, Anabel; Thoonen, Anneleen; Kowalkowski, Lukasz; Gawronski, Stanislaw W.; Rineau, Francois; Vangronsveld, Jaco; Thijs, Sofie
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plants can ‘catch’ and mitigate airborne pollutants and are assisted by fungi inhabiting their leaves. The structure and function of the fungal communities inhabiting the phyllosphere of hornbeam trees growing in two chronically polluted areas, the oilfield of Bóbrka and the city center of Warsaw, were compared to the ones growing in one nature reserve, the Białowieża National Park. Fungi were isolated and characterized both phylogenetically and functionally for their potential role in air pollution mitigation. Both culture-dependent (e.g., enzyme assays and tolerance tests) and culture-independent methods (e.g., ITS and shotgun sequencings) were used. Furthermore, the degradation potential of the fungi was assessed by gas chromatography mass spectrometry (GC-MS). Shotgun sequencing showed that the phyllosphere fungal communities were dominated by fungi belonging to the phylum Ascomycota. Aureobasidium was the only genus detected at the three locations with a relative abundance ≥1.0%. Among the cultivated epiphytic fungi from Bóbrka, Fusarium sporotrichioides AT11, Phoma herbarum AT15, and Lophiostoma sp. AT37 showed in vitro aromatic hydrocarbon degradation potential with laccase activities of 1.24, 3.62, and 7.2 µU L−1, respectively, and peroxidase enzymes with activities of 3.46, 2.28, and 7.49 µU L−1, respectively. Furthermore, Fusarium sporotrichioides AT11 and Phoma herbarum AT15 tolerated exposure to airborne naphthalene and benzene. Lophiostoma sp. AT37 was the most tolerant to exposure to these pollutants, in line with being the best potential aromatic hydrocarbon degrader isolated in this study.
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Portillo Estrada, Miguel. Universiteit Antwerp; Bélgica
Fil: Saran, Anabel. Gobierno de la Provincia de La Pampa. Ministerio Público. Agencia de Investigación Científica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina
Fil: Thoonen, Anneleen. Hasselt University; Bélgica
Fil: Kowalkowski, Lukasz. Hasselt University; Bélgica. Warsaw University of Life Sciences; Polonia
Fil: Gawronski, Stanislaw W.. Warsaw University of Life Sciences; Polonia
Fil: Rineau, Francois. Hasselt University; Bélgica
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
Materia
AIR POLLUTION
FUNGI
PHYLLOREMEDIATION
PHYLLOSPHERE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149952
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/149952
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted AreasImperato, ValeriaPortillo Estrada, MiguelSaran, AnabelThoonen, AnneleenKowalkowski, LukaszGawronski, Stanislaw W.Rineau, FrancoisVangronsveld, JacoThijs, SofieAIR POLLUTIONFUNGIPHYLLOREMEDIATIONPHYLLOSPHEREhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Plants can ‘catch’ and mitigate airborne pollutants and are assisted by fungi inhabiting their leaves. The structure and function of the fungal communities inhabiting the phyllosphere of hornbeam trees growing in two chronically polluted areas, the oilfield of Bóbrka and the city center of Warsaw, were compared to the ones growing in one nature reserve, the Białowieża National Park. Fungi were isolated and characterized both phylogenetically and functionally for their potential role in air pollution mitigation. Both culture-dependent (e.g., enzyme assays and tolerance tests) and culture-independent methods (e.g., ITS and shotgun sequencings) were used. Furthermore, the degradation potential of the fungi was assessed by gas chromatography mass spectrometry (GC-MS). Shotgun sequencing showed that the phyllosphere fungal communities were dominated by fungi belonging to the phylum Ascomycota. Aureobasidium was the only genus detected at the three locations with a relative abundance ≥1.0%. Among the cultivated epiphytic fungi from Bóbrka, Fusarium sporotrichioides AT11, Phoma herbarum AT15, and Lophiostoma sp. AT37 showed in vitro aromatic hydrocarbon degradation potential with laccase activities of 1.24, 3.62, and 7.2 µU L−1, respectively, and peroxidase enzymes with activities of 3.46, 2.28, and 7.49 µU L−1, respectively. Furthermore, Fusarium sporotrichioides AT11 and Phoma herbarum AT15 tolerated exposure to airborne naphthalene and benzene. Lophiostoma sp. AT37 was the most tolerant to exposure to these pollutants, in line with being the best potential aromatic hydrocarbon degrader isolated in this study.Fil: Imperato, Valeria. Hasselt University; BélgicaFil: Portillo Estrada, Miguel. Universiteit Antwerp; BélgicaFil: Saran, Anabel. Gobierno de la Provincia de La Pampa. Ministerio Público. Agencia de Investigación Científica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; ArgentinaFil: Thoonen, Anneleen. Hasselt University; BélgicaFil: Kowalkowski, Lukasz. Hasselt University; Bélgica. Warsaw University of Life Sciences; PoloniaFil: Gawronski, Stanislaw W.. Warsaw University of Life Sciences; PoloniaFil: Rineau, Francois. Hasselt University; BélgicaFil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; PoloniaFil: Thijs, Sofie. Hasselt University; BélgicaMDPI2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149952Imperato, Valeria; Portillo Estrada, Miguel; Saran, Anabel; Thoonen, Anneleen; Kowalkowski, Lukasz; et al.; Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas; MDPI; Journal of Fungi; 7; 11; 11-2021; 1-212309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/7/11/972info:eu-repo/semantics/altIdentifier/doi/10.3390/jof7110972info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:06Zoai:ri.conicet.gov.ar:11336/149952instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:06.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
title Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
spellingShingle Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
Imperato, Valeria
AIR POLLUTION
FUNGI
PHYLLOREMEDIATION
PHYLLOSPHERE
title_short Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
title_full Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
title_fullStr Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
title_full_unstemmed Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
title_sort Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas
dc.creator.none.fl_str_mv Imperato, Valeria
Portillo Estrada, Miguel
Saran, Anabel
Thoonen, Anneleen
Kowalkowski, Lukasz
Gawronski, Stanislaw W.
Rineau, Francois
Vangronsveld, Jaco
Thijs, Sofie
author Imperato, Valeria
author_facet Imperato, Valeria
Portillo Estrada, Miguel
Saran, Anabel
Thoonen, Anneleen
Kowalkowski, Lukasz
Gawronski, Stanislaw W.
Rineau, Francois
Vangronsveld, Jaco
Thijs, Sofie
author_role author
author2 Portillo Estrada, Miguel
Saran, Anabel
Thoonen, Anneleen
Kowalkowski, Lukasz
Gawronski, Stanislaw W.
Rineau, Francois
Vangronsveld, Jaco
Thijs, Sofie
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AIR POLLUTION
FUNGI
PHYLLOREMEDIATION
PHYLLOSPHERE
topic AIR POLLUTION
FUNGI
PHYLLOREMEDIATION
PHYLLOSPHERE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants can ‘catch’ and mitigate airborne pollutants and are assisted by fungi inhabiting their leaves. The structure and function of the fungal communities inhabiting the phyllosphere of hornbeam trees growing in two chronically polluted areas, the oilfield of Bóbrka and the city center of Warsaw, were compared to the ones growing in one nature reserve, the Białowieża National Park. Fungi were isolated and characterized both phylogenetically and functionally for their potential role in air pollution mitigation. Both culture-dependent (e.g., enzyme assays and tolerance tests) and culture-independent methods (e.g., ITS and shotgun sequencings) were used. Furthermore, the degradation potential of the fungi was assessed by gas chromatography mass spectrometry (GC-MS). Shotgun sequencing showed that the phyllosphere fungal communities were dominated by fungi belonging to the phylum Ascomycota. Aureobasidium was the only genus detected at the three locations with a relative abundance ≥1.0%. Among the cultivated epiphytic fungi from Bóbrka, Fusarium sporotrichioides AT11, Phoma herbarum AT15, and Lophiostoma sp. AT37 showed in vitro aromatic hydrocarbon degradation potential with laccase activities of 1.24, 3.62, and 7.2 µU L−1, respectively, and peroxidase enzymes with activities of 3.46, 2.28, and 7.49 µU L−1, respectively. Furthermore, Fusarium sporotrichioides AT11 and Phoma herbarum AT15 tolerated exposure to airborne naphthalene and benzene. Lophiostoma sp. AT37 was the most tolerant to exposure to these pollutants, in line with being the best potential aromatic hydrocarbon degrader isolated in this study.
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Portillo Estrada, Miguel. Universiteit Antwerp; Bélgica
Fil: Saran, Anabel. Gobierno de la Provincia de La Pampa. Ministerio Público. Agencia de Investigación Científica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina
Fil: Thoonen, Anneleen. Hasselt University; Bélgica
Fil: Kowalkowski, Lukasz. Hasselt University; Bélgica. Warsaw University of Life Sciences; Polonia
Fil: Gawronski, Stanislaw W.. Warsaw University of Life Sciences; Polonia
Fil: Rineau, Francois. Hasselt University; Bélgica
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
description Plants can ‘catch’ and mitigate airborne pollutants and are assisted by fungi inhabiting their leaves. The structure and function of the fungal communities inhabiting the phyllosphere of hornbeam trees growing in two chronically polluted areas, the oilfield of Bóbrka and the city center of Warsaw, were compared to the ones growing in one nature reserve, the Białowieża National Park. Fungi were isolated and characterized both phylogenetically and functionally for their potential role in air pollution mitigation. Both culture-dependent (e.g., enzyme assays and tolerance tests) and culture-independent methods (e.g., ITS and shotgun sequencings) were used. Furthermore, the degradation potential of the fungi was assessed by gas chromatography mass spectrometry (GC-MS). Shotgun sequencing showed that the phyllosphere fungal communities were dominated by fungi belonging to the phylum Ascomycota. Aureobasidium was the only genus detected at the three locations with a relative abundance ≥1.0%. Among the cultivated epiphytic fungi from Bóbrka, Fusarium sporotrichioides AT11, Phoma herbarum AT15, and Lophiostoma sp. AT37 showed in vitro aromatic hydrocarbon degradation potential with laccase activities of 1.24, 3.62, and 7.2 µU L−1, respectively, and peroxidase enzymes with activities of 3.46, 2.28, and 7.49 µU L−1, respectively. Furthermore, Fusarium sporotrichioides AT11 and Phoma herbarum AT15 tolerated exposure to airborne naphthalene and benzene. Lophiostoma sp. AT37 was the most tolerant to exposure to these pollutants, in line with being the best potential aromatic hydrocarbon degrader isolated in this study.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149952
Imperato, Valeria; Portillo Estrada, Miguel; Saran, Anabel; Thoonen, Anneleen; Kowalkowski, Lukasz; et al.; Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas; MDPI; Journal of Fungi; 7; 11; 11-2021; 1-21
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149952
identifier_str_mv Imperato, Valeria; Portillo Estrada, Miguel; Saran, Anabel; Thoonen, Anneleen; Kowalkowski, Lukasz; et al.; Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas; MDPI; Journal of Fungi; 7; 11; 11-2021; 1-21
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/7/11/972
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof7110972
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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