Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments

Autores
Marcos, Magalí Silvina; Lozada, Mariana; Dionisi, Hebe Monica
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: The goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (PAH)-degrading bacterial populations in cold marine ecosystems. Methods and Results: We designed a degenerate primer set targeting genes encoding the α subunit of PAH-dioxygenases from Gram-positive bacteria. This primer set was used to amplify gene fragments from metagenomic DNA isolated from Subantarctic marine sediments (Ushuaia Bay, Argentina). These gene fragments were cloned and sequenced. We identified 14 distinct groups of genes, most of them showing significant relatedness with dioxygenases from Gram-positive bacteria of the genera Rhodococcus, Mycobacterium, Nocardioides, Terrabacter and Bacillus. The level of identity with these genes, however, was low to moderate (33-62% at the amino acid level). Conclusion: These results indicate the presence of a high diversity of hitherto unidentified dioxygenase genes in this cold polluted environment. Significance and Impact of the Study: Subantarctic marine ecosystems are particularly vulnerable to hydrocarbon pollution, and the development of environmental restoration strategies for these environments is pressing. The information obtained in this work will be the starting point for the design of quantitative molecular tools to analyse the abundance and dynamics of these aromatic hydrocarbon-degrading bacterial populations in the marine environment.
Fil: Marcos, Magalí Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Materia
DIOXYGENASES
INTERTIDAL SEDIMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
RING-HYDROXYLATING OXYGENASES
SUBANTARCTIC MARINE ENVIRONMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96095
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96095
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sedimentsMarcos, Magalí SilvinaLozada, MarianaDionisi, Hebe MonicaDIOXYGENASESINTERTIDAL SEDIMENTSPOLYCYCLIC AROMATIC HYDROCARBONSRING-HYDROXYLATING OXYGENASESSUBANTARCTIC MARINE ENVIRONMENTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aim: The goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (PAH)-degrading bacterial populations in cold marine ecosystems. Methods and Results: We designed a degenerate primer set targeting genes encoding the α subunit of PAH-dioxygenases from Gram-positive bacteria. This primer set was used to amplify gene fragments from metagenomic DNA isolated from Subantarctic marine sediments (Ushuaia Bay, Argentina). These gene fragments were cloned and sequenced. We identified 14 distinct groups of genes, most of them showing significant relatedness with dioxygenases from Gram-positive bacteria of the genera Rhodococcus, Mycobacterium, Nocardioides, Terrabacter and Bacillus. The level of identity with these genes, however, was low to moderate (33-62% at the amino acid level). Conclusion: These results indicate the presence of a high diversity of hitherto unidentified dioxygenase genes in this cold polluted environment. Significance and Impact of the Study: Subantarctic marine ecosystems are particularly vulnerable to hydrocarbon pollution, and the development of environmental restoration strategies for these environments is pressing. The information obtained in this work will be the starting point for the design of quantitative molecular tools to analyse the abundance and dynamics of these aromatic hydrocarbon-degrading bacterial populations in the marine environment.Fil: Marcos, Magalí Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaWiley Blackwell Publishing, Inc2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96095Marcos, Magalí Silvina; Lozada, Mariana; Dionisi, Hebe Monica; Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 11-2009; 602-6080266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2009.02711.xinfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2009.02711.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:17Zoai:ri.conicet.gov.ar:11336/96095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:17.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
title Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
spellingShingle Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
Marcos, Magalí Silvina
DIOXYGENASES
INTERTIDAL SEDIMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
RING-HYDROXYLATING OXYGENASES
SUBANTARCTIC MARINE ENVIRONMENTS
title_short Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
title_full Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
title_fullStr Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
title_full_unstemmed Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
title_sort Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments
dc.creator.none.fl_str_mv Marcos, Magalí Silvina
Lozada, Mariana
Dionisi, Hebe Monica
author Marcos, Magalí Silvina
author_facet Marcos, Magalí Silvina
Lozada, Mariana
Dionisi, Hebe Monica
author_role author
author2 Lozada, Mariana
Dionisi, Hebe Monica
author2_role author
author
dc.subject.none.fl_str_mv DIOXYGENASES
INTERTIDAL SEDIMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
RING-HYDROXYLATING OXYGENASES
SUBANTARCTIC MARINE ENVIRONMENTS
topic DIOXYGENASES
INTERTIDAL SEDIMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
RING-HYDROXYLATING OXYGENASES
SUBANTARCTIC MARINE ENVIRONMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim: The goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (PAH)-degrading bacterial populations in cold marine ecosystems. Methods and Results: We designed a degenerate primer set targeting genes encoding the α subunit of PAH-dioxygenases from Gram-positive bacteria. This primer set was used to amplify gene fragments from metagenomic DNA isolated from Subantarctic marine sediments (Ushuaia Bay, Argentina). These gene fragments were cloned and sequenced. We identified 14 distinct groups of genes, most of them showing significant relatedness with dioxygenases from Gram-positive bacteria of the genera Rhodococcus, Mycobacterium, Nocardioides, Terrabacter and Bacillus. The level of identity with these genes, however, was low to moderate (33-62% at the amino acid level). Conclusion: These results indicate the presence of a high diversity of hitherto unidentified dioxygenase genes in this cold polluted environment. Significance and Impact of the Study: Subantarctic marine ecosystems are particularly vulnerable to hydrocarbon pollution, and the development of environmental restoration strategies for these environments is pressing. The information obtained in this work will be the starting point for the design of quantitative molecular tools to analyse the abundance and dynamics of these aromatic hydrocarbon-degrading bacterial populations in the marine environment.
Fil: Marcos, Magalí Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
description Aim: The goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (PAH)-degrading bacterial populations in cold marine ecosystems. Methods and Results: We designed a degenerate primer set targeting genes encoding the α subunit of PAH-dioxygenases from Gram-positive bacteria. This primer set was used to amplify gene fragments from metagenomic DNA isolated from Subantarctic marine sediments (Ushuaia Bay, Argentina). These gene fragments were cloned and sequenced. We identified 14 distinct groups of genes, most of them showing significant relatedness with dioxygenases from Gram-positive bacteria of the genera Rhodococcus, Mycobacterium, Nocardioides, Terrabacter and Bacillus. The level of identity with these genes, however, was low to moderate (33-62% at the amino acid level). Conclusion: These results indicate the presence of a high diversity of hitherto unidentified dioxygenase genes in this cold polluted environment. Significance and Impact of the Study: Subantarctic marine ecosystems are particularly vulnerable to hydrocarbon pollution, and the development of environmental restoration strategies for these environments is pressing. The information obtained in this work will be the starting point for the design of quantitative molecular tools to analyse the abundance and dynamics of these aromatic hydrocarbon-degrading bacterial populations in the marine environment.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96095
Marcos, Magalí Silvina; Lozada, Mariana; Dionisi, Hebe Monica; Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 11-2009; 602-608
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96095
identifier_str_mv Marcos, Magalí Silvina; Lozada, Mariana; Dionisi, Hebe Monica; Aromatic hydrocarbon degradation genes from chronically polluted Subantarctic marine sediments; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 11-2009; 602-608
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2009.02711.x
info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2009.02711.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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