Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants

Autores
Pishbin, Fatemehsadat; Mouriño, Viviana Silvia Lourdes; Flor, Sabrina Andrea; Kreppel, Stefan; Salih, Vehid; Ryan, Mary P.; Boccaccini, Aldo R.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.
Fil: Pishbin, Fatemehsadat. Imperial College London; Reino Unido
Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kreppel, Stefan. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania
Fil: Salih, Vehid. University College London; Estados Unidos
Fil: Ryan, Mary P.. Imperial College London; Reino Unido
Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania
Materia
Coatings
Bioactive Glass
Chitosan
Gentamicin
Electrophoretic Deposition
Drug Delivery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15780
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15780
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implantsPishbin, FatemehsadatMouriño, Viviana Silvia LourdesFlor, Sabrina AndreaKreppel, StefanSalih, VehidRyan, Mary P.Boccaccini, Aldo R.CoatingsBioactive GlassChitosanGentamicinElectrophoretic DepositionDrug Deliveryhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.Fil: Pishbin, Fatemehsadat. Imperial College London; Reino UnidoFil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kreppel, Stefan. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; AlemaniaFil: Salih, Vehid. University College London; Estados UnidosFil: Ryan, Mary P.. Imperial College London; Reino UnidoFil: Boccaccini, Aldo R.. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; AlemaniaAmerican Chemical Society2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15780Pishbin, Fatemehsadat; Mouriño, Viviana Silvia Lourdes; Flor, Sabrina Andrea; Kreppel, Stefan; Salih, Vehid; et al.; Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants; American Chemical Society; Acs Applied Materials & Interfaces; 6; 11; 5-2014; 8796-88061944-8244enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/am5014166info:eu-repo/semantics/altIdentifier/doi/10.1021/am5014166info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:22Zoai:ri.conicet.gov.ar:11336/15780instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:23.195CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
title Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
spellingShingle Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
Pishbin, Fatemehsadat
Coatings
Bioactive Glass
Chitosan
Gentamicin
Electrophoretic Deposition
Drug Delivery
title_short Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
title_full Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
title_fullStr Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
title_full_unstemmed Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
title_sort Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants
dc.creator.none.fl_str_mv Pishbin, Fatemehsadat
Mouriño, Viviana Silvia Lourdes
Flor, Sabrina Andrea
Kreppel, Stefan
Salih, Vehid
Ryan, Mary P.
Boccaccini, Aldo R.
author Pishbin, Fatemehsadat
author_facet Pishbin, Fatemehsadat
Mouriño, Viviana Silvia Lourdes
Flor, Sabrina Andrea
Kreppel, Stefan
Salih, Vehid
Ryan, Mary P.
Boccaccini, Aldo R.
author_role author
author2 Mouriño, Viviana Silvia Lourdes
Flor, Sabrina Andrea
Kreppel, Stefan
Salih, Vehid
Ryan, Mary P.
Boccaccini, Aldo R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Coatings
Bioactive Glass
Chitosan
Gentamicin
Electrophoretic Deposition
Drug Delivery
topic Coatings
Bioactive Glass
Chitosan
Gentamicin
Electrophoretic Deposition
Drug Delivery
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.
Fil: Pishbin, Fatemehsadat. Imperial College London; Reino Unido
Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kreppel, Stefan. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania
Fil: Salih, Vehid. University College London; Estados Unidos
Fil: Ryan, Mary P.. Imperial College London; Reino Unido
Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania
description Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15780
Pishbin, Fatemehsadat; Mouriño, Viviana Silvia Lourdes; Flor, Sabrina Andrea; Kreppel, Stefan; Salih, Vehid; et al.; Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants; American Chemical Society; Acs Applied Materials & Interfaces; 6; 11; 5-2014; 8796-8806
1944-8244
url http://hdl.handle.net/11336/15780
identifier_str_mv Pishbin, Fatemehsadat; Mouriño, Viviana Silvia Lourdes; Flor, Sabrina Andrea; Kreppel, Stefan; Salih, Vehid; et al.; Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants; American Chemical Society; Acs Applied Materials & Interfaces; 6; 11; 5-2014; 8796-8806
1944-8244
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/am5014166
info:eu-repo/semantics/altIdentifier/doi/10.1021/am5014166
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083064434786304
score 13.22299

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