Label-free electrochemical detection of singlet oxygen protein damage
- Autores
- Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; Paz Zanini, Veronica Irene; Palecek, Emil; Borsarelli, Claudio Darío; Ostatná, Veronika
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.
Fil: Vargová, Veronika. Czech Academy of Sciences; República Checa
Fil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Cernocka, Hana. Czech Academy of Sciences; República Checa
Fil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Palecek, Emil. Czech Academy of Sciences; República Checa
Fil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Ostatná, Veronika. Czech Academy of Sciences; República Checa - Materia
-
Singlet Oxygen Protein Damage
Surface-Attached Protein Stability
Mercury And Carbon Electrodes
Constant Current Chronopontentiometry
Square Wave Voltammetry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/42386
Ver los metadatos del registro completo
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Label-free electrochemical detection of singlet oxygen protein damageVargová, VeronikaGimenez, Rodrigo EstebanCernocka, HanaChito Trujillo, Diana MariaTulli, Fiorella GiovannaPaz Zanini, Veronica IrenePalecek, EmilBorsarelli, Claudio DaríoOstatná, VeronikaSinglet Oxygen Protein DamageSurface-Attached Protein StabilityMercury And Carbon ElectrodesConstant Current ChronopontentiometrySquare Wave Voltammetryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.Fil: Vargová, Veronika. Czech Academy of Sciences; República ChecaFil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Cernocka, Hana. Czech Academy of Sciences; República ChecaFil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Palecek, Emil. Czech Academy of Sciences; República ChecaFil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Ostatná, Veronika. Czech Academy of Sciences; República ChecaPergamon-Elsevier Science Ltd2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42386Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-6690013-4686CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.11.104info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468615308768info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:53Zoai:ri.conicet.gov.ar:11336/42386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:53.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Label-free electrochemical detection of singlet oxygen protein damage |
title |
Label-free electrochemical detection of singlet oxygen protein damage |
spellingShingle |
Label-free electrochemical detection of singlet oxygen protein damage Vargová, Veronika Singlet Oxygen Protein Damage Surface-Attached Protein Stability Mercury And Carbon Electrodes Constant Current Chronopontentiometry Square Wave Voltammetry |
title_short |
Label-free electrochemical detection of singlet oxygen protein damage |
title_full |
Label-free electrochemical detection of singlet oxygen protein damage |
title_fullStr |
Label-free electrochemical detection of singlet oxygen protein damage |
title_full_unstemmed |
Label-free electrochemical detection of singlet oxygen protein damage |
title_sort |
Label-free electrochemical detection of singlet oxygen protein damage |
dc.creator.none.fl_str_mv |
Vargová, Veronika Gimenez, Rodrigo Esteban Cernocka, Hana Chito Trujillo, Diana Maria Tulli, Fiorella Giovanna Paz Zanini, Veronica Irene Palecek, Emil Borsarelli, Claudio Darío Ostatná, Veronika |
author |
Vargová, Veronika |
author_facet |
Vargová, Veronika Gimenez, Rodrigo Esteban Cernocka, Hana Chito Trujillo, Diana Maria Tulli, Fiorella Giovanna Paz Zanini, Veronica Irene Palecek, Emil Borsarelli, Claudio Darío Ostatná, Veronika |
author_role |
author |
author2 |
Gimenez, Rodrigo Esteban Cernocka, Hana Chito Trujillo, Diana Maria Tulli, Fiorella Giovanna Paz Zanini, Veronica Irene Palecek, Emil Borsarelli, Claudio Darío Ostatná, Veronika |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Singlet Oxygen Protein Damage Surface-Attached Protein Stability Mercury And Carbon Electrodes Constant Current Chronopontentiometry Square Wave Voltammetry |
topic |
Singlet Oxygen Protein Damage Surface-Attached Protein Stability Mercury And Carbon Electrodes Constant Current Chronopontentiometry Square Wave Voltammetry |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages. Fil: Vargová, Veronika. Czech Academy of Sciences; República Checa Fil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina Fil: Cernocka, Hana. Czech Academy of Sciences; República Checa Fil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina Fil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina Fil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina Fil: Palecek, Emil. Czech Academy of Sciences; República Checa Fil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina Fil: Ostatná, Veronika. Czech Academy of Sciences; República Checa |
description |
Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/42386 Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-669 0013-4686 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/42386 |
identifier_str_mv |
Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-669 0013-4686 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.11.104 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468615308768 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613195636932608 |
score |
13.070432 |