Label-free electrochemical detection of singlet oxygen protein damage

Autores
Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; Paz Zanini, Veronica Irene; Palecek, Emil; Borsarelli, Claudio Darío; Ostatná, Veronika
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.
Fil: Vargová, Veronika. Czech Academy of Sciences; República Checa
Fil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Cernocka, Hana. Czech Academy of Sciences; República Checa
Fil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Palecek, Emil. Czech Academy of Sciences; República Checa
Fil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Ostatná, Veronika. Czech Academy of Sciences; República Checa
Materia
Singlet Oxygen Protein Damage
Surface-Attached Protein Stability
Mercury And Carbon Electrodes
Constant Current Chronopontentiometry
Square Wave Voltammetry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42386
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42386
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Label-free electrochemical detection of singlet oxygen protein damageVargová, VeronikaGimenez, Rodrigo EstebanCernocka, HanaChito Trujillo, Diana MariaTulli, Fiorella GiovannaPaz Zanini, Veronica IrenePalecek, EmilBorsarelli, Claudio DaríoOstatná, VeronikaSinglet Oxygen Protein DamageSurface-Attached Protein StabilityMercury And Carbon ElectrodesConstant Current ChronopontentiometrySquare Wave Voltammetryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.Fil: Vargová, Veronika. Czech Academy of Sciences; República ChecaFil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Cernocka, Hana. Czech Academy of Sciences; República ChecaFil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Palecek, Emil. Czech Academy of Sciences; República ChecaFil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Ostatná, Veronika. Czech Academy of Sciences; República ChecaPergamon-Elsevier Science Ltd2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42386Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-6690013-4686CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.11.104info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468615308768info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:06:05Zoai:ri.conicet.gov.ar:11336/42386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:06:05.528CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Label-free electrochemical detection of singlet oxygen protein damage
title Label-free electrochemical detection of singlet oxygen protein damage
spellingShingle Label-free electrochemical detection of singlet oxygen protein damage
Vargová, Veronika
Singlet Oxygen Protein Damage
Surface-Attached Protein Stability
Mercury And Carbon Electrodes
Constant Current Chronopontentiometry
Square Wave Voltammetry
title_short Label-free electrochemical detection of singlet oxygen protein damage
title_full Label-free electrochemical detection of singlet oxygen protein damage
title_fullStr Label-free electrochemical detection of singlet oxygen protein damage
title_full_unstemmed Label-free electrochemical detection of singlet oxygen protein damage
title_sort Label-free electrochemical detection of singlet oxygen protein damage
dc.creator.none.fl_str_mv Vargová, Veronika
Gimenez, Rodrigo Esteban
Cernocka, Hana
Chito Trujillo, Diana Maria
Tulli, Fiorella Giovanna
Paz Zanini, Veronica Irene
Palecek, Emil
Borsarelli, Claudio Darío
Ostatná, Veronika
author Vargová, Veronika
author_facet Vargová, Veronika
Gimenez, Rodrigo Esteban
Cernocka, Hana
Chito Trujillo, Diana Maria
Tulli, Fiorella Giovanna
Paz Zanini, Veronica Irene
Palecek, Emil
Borsarelli, Claudio Darío
Ostatná, Veronika
author_role author
author2 Gimenez, Rodrigo Esteban
Cernocka, Hana
Chito Trujillo, Diana Maria
Tulli, Fiorella Giovanna
Paz Zanini, Veronica Irene
Palecek, Emil
Borsarelli, Claudio Darío
Ostatná, Veronika
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Singlet Oxygen Protein Damage
Surface-Attached Protein Stability
Mercury And Carbon Electrodes
Constant Current Chronopontentiometry
Square Wave Voltammetry
topic Singlet Oxygen Protein Damage
Surface-Attached Protein Stability
Mercury And Carbon Electrodes
Constant Current Chronopontentiometry
Square Wave Voltammetry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.
Fil: Vargová, Veronika. Czech Academy of Sciences; República Checa
Fil: Gimenez, Rodrigo Esteban. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Cernocka, Hana. Czech Academy of Sciences; República Checa
Fil: Chito Trujillo, Diana Maria. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Tulli, Fiorella Giovanna. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Paz Zanini, Veronica Irene. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Palecek, Emil. Czech Academy of Sciences; República Checa
Fil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Ostatná, Veronika. Czech Academy of Sciences; República Checa
description Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (1O2)-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2′-bipyridine)ruthenium(II) cation [Ru(bpy)3]2+ was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42386
Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-669
0013-4686
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42386
identifier_str_mv Vargová, Veronika; Gimenez, Rodrigo Esteban; Cernocka, Hana; Chito Trujillo, Diana Maria; Tulli, Fiorella Giovanna; et al.; Label-free electrochemical detection of singlet oxygen protein damage; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 187; 1-2016; 662-669
0013-4686
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2015.11.104
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468615308768
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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