Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction

Autores
Agudelo Mesa, Leidy Bibiana; Padro, Juan Manuel; Reta, Mario Roberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new sample preparation procedure to determine six heterocyclic aromatic amines (3-Amino-1,4-dimethyl-5H-pirido[4,3-b]indole, 3-Amino-1-methyl-5H-pirido[4,3-b]indole, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine, 2-amino-9H-pyrido-[2,3-b] indole, 2-amino-3-methyl-9H-pyrido-[2,3-b] indole and 2-Amino-1,6-dimethylimidazo [4,5-b]-pyridine) in cooked beefburguers by using a combination of microwave-assisted solvent extraction and dispersive liquid–liquid microextraction with an ionic liquid generated in situ was used. The optimized microwave extraction procedure consisted of a clean-up step with n-heptane and a subsequent dissolution step in basic media to desorb the analytes from the matrix. Next, an aqueous solution of the ionic liquid 1-octyl-3-methylimidazolium tetrafluorborate and sodium hexafluorphosphate was added and a water-insoluble 1-octyl-3-methylimidazolium hexafluorphosphate was formed within the matrix sample. The amines were analyzed by liquid chromatography with fluorescence and diode-array detection by using a typical C18 column. Peak identities were confirmed by absorbance spectral matching. Repeatability (RSD%) between 5.4% and 10.9%, enrichment factors between 19 and 30, limits of detection between 0.35 and 2.4 ng mL−1 and recoveries between 69% and 100% were achieved. The extraction methodology is simple, rapid (about 40 min/sample) cheap and green since small amounts of non-toxic solvents are necessary.
Fil: Agudelo Mesa, Leidy Bibiana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padro, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Heterocyclic Aromatic Amines
Dispersive Liquid-Liquid Microextraction
Ionic Liquids
Beefburguer Samples
Liquid Chromatography
Microwave-Assisted Solvent Extraction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13791
Acceder
id CONICETDig_6e3078a2cd80336caf0f130f7b49fcc6
oai_identifier_str oai:ri.conicet.gov.ar:11336/13791
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextractionAgudelo Mesa, Leidy BibianaPadro, Juan ManuelReta, Mario RobertoHeterocyclic Aromatic AminesDispersive Liquid-Liquid MicroextractionIonic LiquidsBeefburguer SamplesLiquid ChromatographyMicrowave-Assisted Solvent Extractionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new sample preparation procedure to determine six heterocyclic aromatic amines (3-Amino-1,4-dimethyl-5H-pirido[4,3-b]indole, 3-Amino-1-methyl-5H-pirido[4,3-b]indole, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine, 2-amino-9H-pyrido-[2,3-b] indole, 2-amino-3-methyl-9H-pyrido-[2,3-b] indole and 2-Amino-1,6-dimethylimidazo [4,5-b]-pyridine) in cooked beefburguers by using a combination of microwave-assisted solvent extraction and dispersive liquid–liquid microextraction with an ionic liquid generated in situ was used. The optimized microwave extraction procedure consisted of a clean-up step with n-heptane and a subsequent dissolution step in basic media to desorb the analytes from the matrix. Next, an aqueous solution of the ionic liquid 1-octyl-3-methylimidazolium tetrafluorborate and sodium hexafluorphosphate was added and a water-insoluble 1-octyl-3-methylimidazolium hexafluorphosphate was formed within the matrix sample. The amines were analyzed by liquid chromatography with fluorescence and diode-array detection by using a typical C18 column. Peak identities were confirmed by absorbance spectral matching. Repeatability (RSD%) between 5.4% and 10.9%, enrichment factors between 19 and 30, limits of detection between 0.35 and 2.4 ng mL−1 and recoveries between 69% and 100% were achieved. The extraction methodology is simple, rapid (about 40 min/sample) cheap and green since small amounts of non-toxic solvents are necessary.Fil: Agudelo Mesa, Leidy Bibiana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Padro, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13791Agudelo Mesa, Leidy Bibiana; Padro, Juan Manuel; Reta, Mario Roberto; Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction; Elsevier; Food Chemistry; 141; 3; 12-2013; 1694-17010308-8146enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2013.04.076info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814613005220info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:43Zoai:ri.conicet.gov.ar:11336/13791instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:43.317CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
title Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
spellingShingle Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
Agudelo Mesa, Leidy Bibiana
Heterocyclic Aromatic Amines
Dispersive Liquid-Liquid Microextraction
Ionic Liquids
Beefburguer Samples
Liquid Chromatography
Microwave-Assisted Solvent Extraction
title_short Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
title_full Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
title_fullStr Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
title_full_unstemmed Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
title_sort Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction
dc.creator.none.fl_str_mv Agudelo Mesa, Leidy Bibiana
Padro, Juan Manuel
Reta, Mario Roberto
author Agudelo Mesa, Leidy Bibiana
author_facet Agudelo Mesa, Leidy Bibiana
Padro, Juan Manuel
Reta, Mario Roberto
author_role author
author2 Padro, Juan Manuel
Reta, Mario Roberto
author2_role author
author
dc.subject.none.fl_str_mv Heterocyclic Aromatic Amines
Dispersive Liquid-Liquid Microextraction
Ionic Liquids
Beefburguer Samples
Liquid Chromatography
Microwave-Assisted Solvent Extraction
topic Heterocyclic Aromatic Amines
Dispersive Liquid-Liquid Microextraction
Ionic Liquids
Beefburguer Samples
Liquid Chromatography
Microwave-Assisted Solvent Extraction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new sample preparation procedure to determine six heterocyclic aromatic amines (3-Amino-1,4-dimethyl-5H-pirido[4,3-b]indole, 3-Amino-1-methyl-5H-pirido[4,3-b]indole, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine, 2-amino-9H-pyrido-[2,3-b] indole, 2-amino-3-methyl-9H-pyrido-[2,3-b] indole and 2-Amino-1,6-dimethylimidazo [4,5-b]-pyridine) in cooked beefburguers by using a combination of microwave-assisted solvent extraction and dispersive liquid–liquid microextraction with an ionic liquid generated in situ was used. The optimized microwave extraction procedure consisted of a clean-up step with n-heptane and a subsequent dissolution step in basic media to desorb the analytes from the matrix. Next, an aqueous solution of the ionic liquid 1-octyl-3-methylimidazolium tetrafluorborate and sodium hexafluorphosphate was added and a water-insoluble 1-octyl-3-methylimidazolium hexafluorphosphate was formed within the matrix sample. The amines were analyzed by liquid chromatography with fluorescence and diode-array detection by using a typical C18 column. Peak identities were confirmed by absorbance spectral matching. Repeatability (RSD%) between 5.4% and 10.9%, enrichment factors between 19 and 30, limits of detection between 0.35 and 2.4 ng mL−1 and recoveries between 69% and 100% were achieved. The extraction methodology is simple, rapid (about 40 min/sample) cheap and green since small amounts of non-toxic solvents are necessary.
Fil: Agudelo Mesa, Leidy Bibiana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padro, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reta, Mario Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A new sample preparation procedure to determine six heterocyclic aromatic amines (3-Amino-1,4-dimethyl-5H-pirido[4,3-b]indole, 3-Amino-1-methyl-5H-pirido[4,3-b]indole, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine, 2-amino-9H-pyrido-[2,3-b] indole, 2-amino-3-methyl-9H-pyrido-[2,3-b] indole and 2-Amino-1,6-dimethylimidazo [4,5-b]-pyridine) in cooked beefburguers by using a combination of microwave-assisted solvent extraction and dispersive liquid–liquid microextraction with an ionic liquid generated in situ was used. The optimized microwave extraction procedure consisted of a clean-up step with n-heptane and a subsequent dissolution step in basic media to desorb the analytes from the matrix. Next, an aqueous solution of the ionic liquid 1-octyl-3-methylimidazolium tetrafluorborate and sodium hexafluorphosphate was added and a water-insoluble 1-octyl-3-methylimidazolium hexafluorphosphate was formed within the matrix sample. The amines were analyzed by liquid chromatography with fluorescence and diode-array detection by using a typical C18 column. Peak identities were confirmed by absorbance spectral matching. Repeatability (RSD%) between 5.4% and 10.9%, enrichment factors between 19 and 30, limits of detection between 0.35 and 2.4 ng mL−1 and recoveries between 69% and 100% were achieved. The extraction methodology is simple, rapid (about 40 min/sample) cheap and green since small amounts of non-toxic solvents are necessary.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13791
Agudelo Mesa, Leidy Bibiana; Padro, Juan Manuel; Reta, Mario Roberto; Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction; Elsevier; Food Chemistry; 141; 3; 12-2013; 1694-1701
0308-8146
url http://hdl.handle.net/11336/13791
identifier_str_mv Agudelo Mesa, Leidy Bibiana; Padro, Juan Manuel; Reta, Mario Roberto; Analysis of non-polar heterocyclic aromatic amines in beefburguers by using microwave-assisted extraction and dispersive liquid–ionic liquid microextraction; Elsevier; Food Chemistry; 141; 3; 12-2013; 1694-1701
0308-8146
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2013.04.076
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0308814613005220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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