Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement

Autores
Leasen, Suthisa; Hodak, Jose Hector; Srisala, Jiraporn; Srikhirin, Toemsak; Sritunyalucksana, Kallaya; Veerasai, Waret; Dangtip, Somsak
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.
Fil: Leasen, Suthisa . Mahidol University. Faculty of Sciences; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Srisala, Jiraporn. Mahidol University. Faculty of Science; Tailandia
Fil: Srikhirin, Toemsak . Mahidol University. Faculty of Science; Tailandia
Fil: Sritunyalucksana, Kallaya . National Science and Technology Development Agency; Tailandia
Fil: Veerasai, Waret . Mahidol University. Faculty of Science; Tailandia
Fil: Dangtip, Somsak . Mahidol University. Faculty of Science; Tailandia
Materia
Hybridization
Tin-Doped Indium Oxide
3-Mercaptopropionic Acid
Differential Pulse Voltammetry
Methylene Blue
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8401
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8401
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurementLeasen, Suthisa Hodak, Jose Hector Srisala, JirapornSrikhirin, Toemsak Sritunyalucksana, Kallaya Veerasai, Waret Dangtip, Somsak HybridizationTin-Doped Indium Oxide3-Mercaptopropionic AcidDifferential Pulse VoltammetryMethylene Bluehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.Fil: Leasen, Suthisa . Mahidol University. Faculty of Sciences; TailandiaFil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Srisala, Jiraporn. Mahidol University. Faculty of Science; TailandiaFil: Srikhirin, Toemsak . Mahidol University. Faculty of Science; TailandiaFil: Sritunyalucksana, Kallaya . National Science and Technology Development Agency; TailandiaFil: Veerasai, Waret . Mahidol University. Faculty of Science; TailandiaFil: Dangtip, Somsak . Mahidol University. Faculty of Science; TailandiaScientific.Net2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8401Leasen, Suthisa ; Hodak, Jose Hector; Srisala, Jiraporn; Srikhirin, Toemsak ; Sritunyalucksana, Kallaya ; et al.; Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement; Scientific.Net; Advanced Materials Research; 770; 9-2013; 402-4081662-8985enginfo:eu-repo/semantics/altIdentifier/url/http://www.scientific.net.sci-hub.org/AMR.770.402info:eu-repo/semantics/altIdentifier/doi/10.4028/www.scientific.net/AMR.770.402info:eu-repo/semantics/altIdentifier/hdl/http://ir.sc.mahidol.ac.th/handle/123456789/76info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:57Zoai:ri.conicet.gov.ar:11336/8401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:57.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
title Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
spellingShingle Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
Leasen, Suthisa
Hybridization
Tin-Doped Indium Oxide
3-Mercaptopropionic Acid
Differential Pulse Voltammetry
Methylene Blue
title_short Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
title_full Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
title_fullStr Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
title_full_unstemmed Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
title_sort Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement
dc.creator.none.fl_str_mv Leasen, Suthisa
Hodak, Jose Hector
Srisala, Jiraporn
Srikhirin, Toemsak
Sritunyalucksana, Kallaya
Veerasai, Waret
Dangtip, Somsak
author Leasen, Suthisa
author_facet Leasen, Suthisa
Hodak, Jose Hector
Srisala, Jiraporn
Srikhirin, Toemsak
Sritunyalucksana, Kallaya
Veerasai, Waret
Dangtip, Somsak
author_role author
author2 Hodak, Jose Hector
Srisala, Jiraporn
Srikhirin, Toemsak
Sritunyalucksana, Kallaya
Veerasai, Waret
Dangtip, Somsak
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hybridization
Tin-Doped Indium Oxide
3-Mercaptopropionic Acid
Differential Pulse Voltammetry
Methylene Blue
topic Hybridization
Tin-Doped Indium Oxide
3-Mercaptopropionic Acid
Differential Pulse Voltammetry
Methylene Blue
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.
Fil: Leasen, Suthisa . Mahidol University. Faculty of Sciences; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Srisala, Jiraporn. Mahidol University. Faculty of Science; Tailandia
Fil: Srikhirin, Toemsak . Mahidol University. Faculty of Science; Tailandia
Fil: Sritunyalucksana, Kallaya . National Science and Technology Development Agency; Tailandia
Fil: Veerasai, Waret . Mahidol University. Faculty of Science; Tailandia
Fil: Dangtip, Somsak . Mahidol University. Faculty of Science; Tailandia
description Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8401
Leasen, Suthisa ; Hodak, Jose Hector; Srisala, Jiraporn; Srikhirin, Toemsak ; Sritunyalucksana, Kallaya ; et al.; Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement; Scientific.Net; Advanced Materials Research; 770; 9-2013; 402-408
1662-8985
url http://hdl.handle.net/11336/8401
identifier_str_mv Leasen, Suthisa ; Hodak, Jose Hector; Srisala, Jiraporn; Srikhirin, Toemsak ; Sritunyalucksana, Kallaya ; et al.; Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement; Scientific.Net; Advanced Materials Research; 770; 9-2013; 402-408
1662-8985
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scientific.net.sci-hub.org/AMR.770.402
info:eu-repo/semantics/altIdentifier/doi/10.4028/www.scientific.net/AMR.770.402
info:eu-repo/semantics/altIdentifier/hdl/http://ir.sc.mahidol.ac.th/handle/123456789/76
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific.Net
publisher.none.fl_str_mv Scientific.Net
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781250181791744
score 12.982451

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