Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol

Autores
Castro, Gerardo Daniel; Delgado, A. M. A.; Fanelli, Silvia Laura; Maciel, Maria Eugenia; Diaz Gomez, Maria Isabel; Castro, Jose Alberto
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies reported the presence in rat mammary tissue of a cytosolic xanthine oxidoreductase pathway for the metabolism of alcohol to acetaldehyde and hydroxyl radicals and to the microsomal biotransformation of ethanol to acetaldehyde. It was also reported that after chronic ethanol drinking stressful oxidative conditions can be observed. The present work reports that even after single doses of ethanol, given at three different levels (6.3 g kg(-1); 3.8 g kg(-1) or 0.6 g kg(-1) p.o.), acetaldehyde accumulates for prolonged periods of time in the mammary tissue to reach concentrations higher than in blood (e.g. 5.1 +/- 1.2 nmol g(-1) versus 0.2 +/- 0.1 nmol ml(-1), for 6.3 g kg(-1) dose, 6 h after intoxication). The presence in rat mammary tissue of low activities of additional enzymes able to generate acetaldehyde was established (alcohol dehydrogenase: 0.97 +/- 0.84 mU mg(-1) protein; CYP2E1: 1.30 +/- 0.12 x 10(-2) pmol 4-nitrocatechol min(-1) mg(-1) protein) and a low activity of aldehyde dehydrogenase was observed in the cytosolic, mitochondrial and microsomal fractions (0.02 +/- 0.04; 0.35 +/- 0.09 and 0.72 +/- 0.19 mU mg(-1) protein, respectively). After a single high dose of ethanol, an increased susceptibility to oxidative stress was observed, as evidenced by changes in the shape of t-butylhydroperoxide induced emission of chemiluminescence in mammary tissue (6.3 g kg(-1) dose; at 3 and 6 h). In summary, the results show that even after single doses of ethanol, acetaldehyde, either formed in situ or arriving via blood, tends to accumulate in mammary tissue and that this condition might decrease cell defenses against injury.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Delgado, A. M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Maciel, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Materia
Acetaldehyde
Mammary Tissue
Ethanol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25608
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25608
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcoholCastro, Gerardo DanielDelgado, A. M. A.Fanelli, Silvia LauraMaciel, Maria EugeniaDiaz Gomez, Maria IsabelCastro, Jose AlbertoAcetaldehydeMammary TissueEthanolhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous studies reported the presence in rat mammary tissue of a cytosolic xanthine oxidoreductase pathway for the metabolism of alcohol to acetaldehyde and hydroxyl radicals and to the microsomal biotransformation of ethanol to acetaldehyde. It was also reported that after chronic ethanol drinking stressful oxidative conditions can be observed. The present work reports that even after single doses of ethanol, given at three different levels (6.3 g kg(-1); 3.8 g kg(-1) or 0.6 g kg(-1) p.o.), acetaldehyde accumulates for prolonged periods of time in the mammary tissue to reach concentrations higher than in blood (e.g. 5.1 +/- 1.2 nmol g(-1) versus 0.2 +/- 0.1 nmol ml(-1), for 6.3 g kg(-1) dose, 6 h after intoxication). The presence in rat mammary tissue of low activities of additional enzymes able to generate acetaldehyde was established (alcohol dehydrogenase: 0.97 +/- 0.84 mU mg(-1) protein; CYP2E1: 1.30 +/- 0.12 x 10(-2) pmol 4-nitrocatechol min(-1) mg(-1) protein) and a low activity of aldehyde dehydrogenase was observed in the cytosolic, mitochondrial and microsomal fractions (0.02 +/- 0.04; 0.35 +/- 0.09 and 0.72 +/- 0.19 mU mg(-1) protein, respectively). After a single high dose of ethanol, an increased susceptibility to oxidative stress was observed, as evidenced by changes in the shape of t-butylhydroperoxide induced emission of chemiluminescence in mammary tissue (6.3 g kg(-1) dose; at 3 and 6 h). In summary, the results show that even after single doses of ethanol, acetaldehyde, either formed in situ or arriving via blood, tends to accumulate in mammary tissue and that this condition might decrease cell defenses against injury.Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Delgado, A. M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Maciel, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaWiley2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25608Castro, Gerardo Daniel; Delgado, A. M. A.; Fanelli, Silvia Laura; Maciel, Maria Eugenia; Diaz Gomez, Maria Isabel; et al.; Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol; Wiley; Journal of Applied Toxicology; 28; 3; 6-2007; 315-3210260-437XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jat.1281info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.1281/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:43:10Zoai:ri.conicet.gov.ar:11336/25608instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:43:10.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
title Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
spellingShingle Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
Castro, Gerardo Daniel
Acetaldehyde
Mammary Tissue
Ethanol
title_short Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
title_full Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
title_fullStr Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
title_full_unstemmed Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
title_sort Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol
dc.creator.none.fl_str_mv Castro, Gerardo Daniel
Delgado, A. M. A.
Fanelli, Silvia Laura
Maciel, Maria Eugenia
Diaz Gomez, Maria Isabel
Castro, Jose Alberto
author Castro, Gerardo Daniel
author_facet Castro, Gerardo Daniel
Delgado, A. M. A.
Fanelli, Silvia Laura
Maciel, Maria Eugenia
Diaz Gomez, Maria Isabel
Castro, Jose Alberto
author_role author
author2 Delgado, A. M. A.
Fanelli, Silvia Laura
Maciel, Maria Eugenia
Diaz Gomez, Maria Isabel
Castro, Jose Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Acetaldehyde
Mammary Tissue
Ethanol
topic Acetaldehyde
Mammary Tissue
Ethanol
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous studies reported the presence in rat mammary tissue of a cytosolic xanthine oxidoreductase pathway for the metabolism of alcohol to acetaldehyde and hydroxyl radicals and to the microsomal biotransformation of ethanol to acetaldehyde. It was also reported that after chronic ethanol drinking stressful oxidative conditions can be observed. The present work reports that even after single doses of ethanol, given at three different levels (6.3 g kg(-1); 3.8 g kg(-1) or 0.6 g kg(-1) p.o.), acetaldehyde accumulates for prolonged periods of time in the mammary tissue to reach concentrations higher than in blood (e.g. 5.1 +/- 1.2 nmol g(-1) versus 0.2 +/- 0.1 nmol ml(-1), for 6.3 g kg(-1) dose, 6 h after intoxication). The presence in rat mammary tissue of low activities of additional enzymes able to generate acetaldehyde was established (alcohol dehydrogenase: 0.97 +/- 0.84 mU mg(-1) protein; CYP2E1: 1.30 +/- 0.12 x 10(-2) pmol 4-nitrocatechol min(-1) mg(-1) protein) and a low activity of aldehyde dehydrogenase was observed in the cytosolic, mitochondrial and microsomal fractions (0.02 +/- 0.04; 0.35 +/- 0.09 and 0.72 +/- 0.19 mU mg(-1) protein, respectively). After a single high dose of ethanol, an increased susceptibility to oxidative stress was observed, as evidenced by changes in the shape of t-butylhydroperoxide induced emission of chemiluminescence in mammary tissue (6.3 g kg(-1) dose; at 3 and 6 h). In summary, the results show that even after single doses of ethanol, acetaldehyde, either formed in situ or arriving via blood, tends to accumulate in mammary tissue and that this condition might decrease cell defenses against injury.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Delgado, A. M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Maciel, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
description Previous studies reported the presence in rat mammary tissue of a cytosolic xanthine oxidoreductase pathway for the metabolism of alcohol to acetaldehyde and hydroxyl radicals and to the microsomal biotransformation of ethanol to acetaldehyde. It was also reported that after chronic ethanol drinking stressful oxidative conditions can be observed. The present work reports that even after single doses of ethanol, given at three different levels (6.3 g kg(-1); 3.8 g kg(-1) or 0.6 g kg(-1) p.o.), acetaldehyde accumulates for prolonged periods of time in the mammary tissue to reach concentrations higher than in blood (e.g. 5.1 +/- 1.2 nmol g(-1) versus 0.2 +/- 0.1 nmol ml(-1), for 6.3 g kg(-1) dose, 6 h after intoxication). The presence in rat mammary tissue of low activities of additional enzymes able to generate acetaldehyde was established (alcohol dehydrogenase: 0.97 +/- 0.84 mU mg(-1) protein; CYP2E1: 1.30 +/- 0.12 x 10(-2) pmol 4-nitrocatechol min(-1) mg(-1) protein) and a low activity of aldehyde dehydrogenase was observed in the cytosolic, mitochondrial and microsomal fractions (0.02 +/- 0.04; 0.35 +/- 0.09 and 0.72 +/- 0.19 mU mg(-1) protein, respectively). After a single high dose of ethanol, an increased susceptibility to oxidative stress was observed, as evidenced by changes in the shape of t-butylhydroperoxide induced emission of chemiluminescence in mammary tissue (6.3 g kg(-1) dose; at 3 and 6 h). In summary, the results show that even after single doses of ethanol, acetaldehyde, either formed in situ or arriving via blood, tends to accumulate in mammary tissue and that this condition might decrease cell defenses against injury.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25608
Castro, Gerardo Daniel; Delgado, A. M. A.; Fanelli, Silvia Laura; Maciel, Maria Eugenia; Diaz Gomez, Maria Isabel; et al.; Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol; Wiley; Journal of Applied Toxicology; 28; 3; 6-2007; 315-321
0260-437X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25608
identifier_str_mv Castro, Gerardo Daniel; Delgado, A. M. A.; Fanelli, Silvia Laura; Maciel, Maria Eugenia; Diaz Gomez, Maria Isabel; et al.; Acetaldehyde accumulation in rat mammary tissue after an acute treatment with alcohol; Wiley; Journal of Applied Toxicology; 28; 3; 6-2007; 315-321
0260-437X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jat.1281
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.1281/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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