Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration

Autores
Adler, Catalina; Monavari, Mahshid; Abraham, Gustavo Abel; Boccaccini, Aldo R.; Ghorbani, Farnaz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study seeks to simulate both the chemistry and piezoelectricity of bone by synthesizing electroconductive silane-modified gelatin-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) scaffolds using the freeze drying technique. In order to enhance hydrophilicity, cell interaction, and biomineralization, the scaffolds were functionalized with polydopamine (PDA) inspired by mussels. Physicochemical, electrical, and mechanical analyses were conducted on the scaffolds, as well as in vitro evaluations using the osteosarcoma cell line MG-63. It was found that scaffolds had interconnected porous structures, so the PDA layer formation reduced the size of pores while maintaining scaffold uniformity. PDA functionalization reduced the electrical resistance of the constructs while improving their hydrophilicity, compressive strength, and modulus. As a result of the PDA functionalization and the use of silane coupling agents, higher stability and durability were achieved as well as an improvement in biomineralization capability after being soaked in SBF solution for a month. Additionally, the PDA coating enabled the constructs to enhance viability, adhesion, and proliferation of MG-63 cells, as well as to express alkaline phosphatase and deposit HA, indicating that scaffolds can be used for bone regeneration. Therefore, the PDA-coated scaffolds developed in this study and the non-toxic performance of PEDOT:PSS present a promising approach for further in vitro and in vivo studies.
Fil: Adler, Catalina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universitat Erlangen-Nuremberg; Alemania
Fil: Monavari, Mahshid. Universitat Erlangen-Nuremberg; Alemania
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Ghorbani, Farnaz. Universitat Erlangen-Nuremberg; Alemania
Materia
POLYMER SCAFFOLDS
BONE TISSUE ENGINEERING
PIEZOELECTRICITY
POROUS STRUCTURES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278187
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/278187
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regenerationAdler, CatalinaMonavari, MahshidAbraham, Gustavo AbelBoccaccini, Aldo R.Ghorbani, FarnazPOLYMER SCAFFOLDSBONE TISSUE ENGINEERINGPIEZOELECTRICITYPOROUS STRUCTUREShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This study seeks to simulate both the chemistry and piezoelectricity of bone by synthesizing electroconductive silane-modified gelatin-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) scaffolds using the freeze drying technique. In order to enhance hydrophilicity, cell interaction, and biomineralization, the scaffolds were functionalized with polydopamine (PDA) inspired by mussels. Physicochemical, electrical, and mechanical analyses were conducted on the scaffolds, as well as in vitro evaluations using the osteosarcoma cell line MG-63. It was found that scaffolds had interconnected porous structures, so the PDA layer formation reduced the size of pores while maintaining scaffold uniformity. PDA functionalization reduced the electrical resistance of the constructs while improving their hydrophilicity, compressive strength, and modulus. As a result of the PDA functionalization and the use of silane coupling agents, higher stability and durability were achieved as well as an improvement in biomineralization capability after being soaked in SBF solution for a month. Additionally, the PDA coating enabled the constructs to enhance viability, adhesion, and proliferation of MG-63 cells, as well as to express alkaline phosphatase and deposit HA, indicating that scaffolds can be used for bone regeneration. Therefore, the PDA-coated scaffolds developed in this study and the non-toxic performance of PEDOT:PSS present a promising approach for further in vitro and in vivo studies.Fil: Adler, Catalina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universitat Erlangen-Nuremberg; AlemaniaFil: Monavari, Mahshid. Universitat Erlangen-Nuremberg; AlemaniaFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Ghorbani, Farnaz. Universitat Erlangen-Nuremberg; AlemaniaRoyal Society of Chemistry2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278187Adler, Catalina; Monavari, Mahshid; Abraham, Gustavo Abel; Boccaccini, Aldo R.; Ghorbani, Farnaz; Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration; Royal Society of Chemistry; RSC Advances; 13; 23; 5-2023; 15960-159742046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra01311ainfo:eu-repo/semantics/altIdentifier/doi/10.1039/D3RA01311Ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:28:12Zoai:ri.conicet.gov.ar:11336/278187instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:28:13.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
title Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
spellingShingle Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
Adler, Catalina
POLYMER SCAFFOLDS
BONE TISSUE ENGINEERING
PIEZOELECTRICITY
POROUS STRUCTURES
title_short Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
title_full Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
title_fullStr Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
title_full_unstemmed Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
title_sort Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration
dc.creator.none.fl_str_mv Adler, Catalina
Monavari, Mahshid
Abraham, Gustavo Abel
Boccaccini, Aldo R.
Ghorbani, Farnaz
author Adler, Catalina
author_facet Adler, Catalina
Monavari, Mahshid
Abraham, Gustavo Abel
Boccaccini, Aldo R.
Ghorbani, Farnaz
author_role author
author2 Monavari, Mahshid
Abraham, Gustavo Abel
Boccaccini, Aldo R.
Ghorbani, Farnaz
author2_role author
author
author
author
dc.subject.none.fl_str_mv POLYMER SCAFFOLDS
BONE TISSUE ENGINEERING
PIEZOELECTRICITY
POROUS STRUCTURES
topic POLYMER SCAFFOLDS
BONE TISSUE ENGINEERING
PIEZOELECTRICITY
POROUS STRUCTURES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This study seeks to simulate both the chemistry and piezoelectricity of bone by synthesizing electroconductive silane-modified gelatin-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) scaffolds using the freeze drying technique. In order to enhance hydrophilicity, cell interaction, and biomineralization, the scaffolds were functionalized with polydopamine (PDA) inspired by mussels. Physicochemical, electrical, and mechanical analyses were conducted on the scaffolds, as well as in vitro evaluations using the osteosarcoma cell line MG-63. It was found that scaffolds had interconnected porous structures, so the PDA layer formation reduced the size of pores while maintaining scaffold uniformity. PDA functionalization reduced the electrical resistance of the constructs while improving their hydrophilicity, compressive strength, and modulus. As a result of the PDA functionalization and the use of silane coupling agents, higher stability and durability were achieved as well as an improvement in biomineralization capability after being soaked in SBF solution for a month. Additionally, the PDA coating enabled the constructs to enhance viability, adhesion, and proliferation of MG-63 cells, as well as to express alkaline phosphatase and deposit HA, indicating that scaffolds can be used for bone regeneration. Therefore, the PDA-coated scaffolds developed in this study and the non-toxic performance of PEDOT:PSS present a promising approach for further in vitro and in vivo studies.
Fil: Adler, Catalina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Universitat Erlangen-Nuremberg; Alemania
Fil: Monavari, Mahshid. Universitat Erlangen-Nuremberg; Alemania
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Ghorbani, Farnaz. Universitat Erlangen-Nuremberg; Alemania
description This study seeks to simulate both the chemistry and piezoelectricity of bone by synthesizing electroconductive silane-modified gelatin-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) scaffolds using the freeze drying technique. In order to enhance hydrophilicity, cell interaction, and biomineralization, the scaffolds were functionalized with polydopamine (PDA) inspired by mussels. Physicochemical, electrical, and mechanical analyses were conducted on the scaffolds, as well as in vitro evaluations using the osteosarcoma cell line MG-63. It was found that scaffolds had interconnected porous structures, so the PDA layer formation reduced the size of pores while maintaining scaffold uniformity. PDA functionalization reduced the electrical resistance of the constructs while improving their hydrophilicity, compressive strength, and modulus. As a result of the PDA functionalization and the use of silane coupling agents, higher stability and durability were achieved as well as an improvement in biomineralization capability after being soaked in SBF solution for a month. Additionally, the PDA coating enabled the constructs to enhance viability, adhesion, and proliferation of MG-63 cells, as well as to express alkaline phosphatase and deposit HA, indicating that scaffolds can be used for bone regeneration. Therefore, the PDA-coated scaffolds developed in this study and the non-toxic performance of PEDOT:PSS present a promising approach for further in vitro and in vivo studies.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278187
Adler, Catalina; Monavari, Mahshid; Abraham, Gustavo Abel; Boccaccini, Aldo R.; Ghorbani, Farnaz; Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration; Royal Society of Chemistry; RSC Advances; 13; 23; 5-2023; 15960-15974
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278187
identifier_str_mv Adler, Catalina; Monavari, Mahshid; Abraham, Gustavo Abel; Boccaccini, Aldo R.; Ghorbani, Farnaz; Mussel-inspired polydopamine decorated silane modified-electroconductive gelatin-PEDOT:PSS scaffolds for bone regeneration; Royal Society of Chemistry; RSC Advances; 13; 23; 5-2023; 15960-15974
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra01311a
info:eu-repo/semantics/altIdentifier/doi/10.1039/D3RA01311A
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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