Differential Role of the T6SS in Acinetobacter baumannii Virulence

Autores
Repizo, Guillermo Daniel; Gagné, Stéphanie; Foucault Grunenwald, Marie Laure; Borges, Vitor; Charpentier, Xavier; Limansky, Adriana Sara; Gomes, João Paulo; Viale, Alejandro Miguel; Salcedo, Suzana P.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.
Fil: Repizo, Guillermo Daniel. Université Claude Bernard Lyon 1; Francia
Fil: Gagné, Stéphanie. Université Claude Bernard Lyon 1; Francia
Fil: Foucault Grunenwald, Marie Laure. Université Claude Bernard Lyon 1; Francia
Fil: Borges, Vitor. National Institute of Health; Portugal
Fil: Charpentier, Xavier. Université Claude Bernard Lyon 1; Francia
Fil: Limansky, Adriana Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gomes, João Paulo. National Institute of Health; Portugal
Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Salcedo, Suzana P.. Université Claude Bernard Lyon 1; Francia
Materia
Acinetobacter baumannii
Virulence
T6SS
Bacterial dueling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21530
Acceder
id CONICETDig_6c70ab1dc1e6c65855580bf8cb071d41
oai_identifier_str oai:ri.conicet.gov.ar:11336/21530
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential Role of the T6SS in Acinetobacter baumannii VirulenceRepizo, Guillermo DanielGagné, StéphanieFoucault Grunenwald, Marie LaureBorges, VitorCharpentier, XavierLimansky, Adriana SaraGomes, João PauloViale, Alejandro MiguelSalcedo, Suzana P.Acinetobacter baumanniiVirulenceT6SSBacterial duelinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.Fil: Repizo, Guillermo Daniel. Université Claude Bernard Lyon 1; FranciaFil: Gagné, Stéphanie. Université Claude Bernard Lyon 1; FranciaFil: Foucault Grunenwald, Marie Laure. Université Claude Bernard Lyon 1; FranciaFil: Borges, Vitor. National Institute of Health; PortugalFil: Charpentier, Xavier. Université Claude Bernard Lyon 1; FranciaFil: Limansky, Adriana Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gomes, João Paulo. National Institute of Health; PortugalFil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Salcedo, Suzana P.. Université Claude Bernard Lyon 1; FranciaPublic Library Of Science2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21530Repizo, Guillermo Daniel; Gagné, Stéphanie; Foucault Grunenwald, Marie Laure; Borges, Vitor; Charpentier, Xavier; et al.; Differential Role of the T6SS in Acinetobacter baumannii Virulence; Public Library Of Science; Plos One; 10; 9; 9-2015; 1-20; e01382651932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0138265info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138265info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:50Zoai:ri.conicet.gov.ar:11336/21530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:50.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential Role of the T6SS in Acinetobacter baumannii Virulence
title Differential Role of the T6SS in Acinetobacter baumannii Virulence
spellingShingle Differential Role of the T6SS in Acinetobacter baumannii Virulence
Repizo, Guillermo Daniel
Acinetobacter baumannii
Virulence
T6SS
Bacterial dueling
title_short Differential Role of the T6SS in Acinetobacter baumannii Virulence
title_full Differential Role of the T6SS in Acinetobacter baumannii Virulence
title_fullStr Differential Role of the T6SS in Acinetobacter baumannii Virulence
title_full_unstemmed Differential Role of the T6SS in Acinetobacter baumannii Virulence
title_sort Differential Role of the T6SS in Acinetobacter baumannii Virulence
dc.creator.none.fl_str_mv Repizo, Guillermo Daniel
Gagné, Stéphanie
Foucault Grunenwald, Marie Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana Sara
Gomes, João Paulo
Viale, Alejandro Miguel
Salcedo, Suzana P.
author Repizo, Guillermo Daniel
author_facet Repizo, Guillermo Daniel
Gagné, Stéphanie
Foucault Grunenwald, Marie Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana Sara
Gomes, João Paulo
Viale, Alejandro Miguel
Salcedo, Suzana P.
author_role author
author2 Gagné, Stéphanie
Foucault Grunenwald, Marie Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana Sara
Gomes, João Paulo
Viale, Alejandro Miguel
Salcedo, Suzana P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acinetobacter baumannii
Virulence
T6SS
Bacterial dueling
topic Acinetobacter baumannii
Virulence
T6SS
Bacterial dueling
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.
Fil: Repizo, Guillermo Daniel. Université Claude Bernard Lyon 1; Francia
Fil: Gagné, Stéphanie. Université Claude Bernard Lyon 1; Francia
Fil: Foucault Grunenwald, Marie Laure. Université Claude Bernard Lyon 1; Francia
Fil: Borges, Vitor. National Institute of Health; Portugal
Fil: Charpentier, Xavier. Université Claude Bernard Lyon 1; Francia
Fil: Limansky, Adriana Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gomes, João Paulo. National Institute of Health; Portugal
Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Salcedo, Suzana P.. Université Claude Bernard Lyon 1; Francia
description Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21530
Repizo, Guillermo Daniel; Gagné, Stéphanie; Foucault Grunenwald, Marie Laure; Borges, Vitor; Charpentier, Xavier; et al.; Differential Role of the T6SS in Acinetobacter baumannii Virulence; Public Library Of Science; Plos One; 10; 9; 9-2015; 1-20; e0138265
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21530
identifier_str_mv Repizo, Guillermo Daniel; Gagné, Stéphanie; Foucault Grunenwald, Marie Laure; Borges, Vitor; Charpentier, Xavier; et al.; Differential Role of the T6SS in Acinetobacter baumannii Virulence; Public Library Of Science; Plos One; 10; 9; 9-2015; 1-20; e0138265
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0138265
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138265
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614317479034880
score 13.070432

Publicaciones similares