Genomic organization of the 5′ region of the human thyroglobulin gene

Autores
Moya, C. M.; Mendive, F. M.; Rivolta, Carina Marcela; Vassart, G.; Targovnik, Hector Manuel
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The purpose of the present work is to establish the intron - exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5′ terminal half of the gene. Design: Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. Methods: A human genomic DNA library was screened by in situ hybridization. Southern blotting experiments were performed to characterize the phage inserts. Intron/exon junction sequences were determined by the Taq polymerase-based chain terminator method. Finally, the thyroglobulin gene was mapped using the Gene Bridge 4 radiation hybrid clone panel. Results: We isolated and characterized four λ phage clones that include nucleotides 3002 to 4816 of the thyroglobulin mRNA, encompassing exons 12 to 23 of the gene. The exon sizes range between 78 and 219 nucleotides. We found that the GT-AG splicing sequences rule was perfectly respected in all the introns. A total of 7302 intronic bases was analyzed. Hormogenic tyrosine 5 and 1291 are encoded by exons 2 and 18. Also, seven alternative spliced variants are associated with the 5′ region. Thyroglobulin gene maps to 5.5 centiRays from the AFMAO53XF1 marker, in chromosome 8. Conclusions: The present study shows that the first 4857 bases of thyroglobulin mRNA are divided into 23 exons and the four phages isolated include 32.6 kb genomic DNA, covering 1815 nucleotides of exonic sequence distributed in 12 exons, from exon 12 to 23.
Fil: Moya, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mendive, F. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Rivolta, Carina Marcela. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vassart, G.. Université Libre de Bruxelles; Bélgica
Fil: Targovnik, Hector Manuel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Université Libre de Bruxelles; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Genomic Organization
Thyroglobulin Gene
5' Region
Exon/Intron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40328
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40328
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic organization of the 5′ region of the human thyroglobulin geneMoya, C. M.Mendive, F. M.Rivolta, Carina MarcelaVassart, G.Targovnik, Hector ManuelGenomic OrganizationThyroglobulin Gene5' RegionExon/Intronhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: The purpose of the present work is to establish the intron - exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5′ terminal half of the gene. Design: Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. Methods: A human genomic DNA library was screened by in situ hybridization. Southern blotting experiments were performed to characterize the phage inserts. Intron/exon junction sequences were determined by the Taq polymerase-based chain terminator method. Finally, the thyroglobulin gene was mapped using the Gene Bridge 4 radiation hybrid clone panel. Results: We isolated and characterized four λ phage clones that include nucleotides 3002 to 4816 of the thyroglobulin mRNA, encompassing exons 12 to 23 of the gene. The exon sizes range between 78 and 219 nucleotides. We found that the GT-AG splicing sequences rule was perfectly respected in all the introns. A total of 7302 intronic bases was analyzed. Hormogenic tyrosine 5 and 1291 are encoded by exons 2 and 18. Also, seven alternative spliced variants are associated with the 5′ region. Thyroglobulin gene maps to 5.5 centiRays from the AFMAO53XF1 marker, in chromosome 8. Conclusions: The present study shows that the first 4857 bases of thyroglobulin mRNA are divided into 23 exons and the four phages isolated include 32.6 kb genomic DNA, covering 1815 nucleotides of exonic sequence distributed in 12 exons, from exon 12 to 23.Fil: Moya, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Mendive, F. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Rivolta, Carina Marcela. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vassart, G.. Université Libre de Bruxelles; BélgicaFil: Targovnik, Hector Manuel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Université Libre de Bruxelles; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioScientifica2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40328Moya, C. M.; Mendive, F. M.; Rivolta, Carina Marcela; Vassart, G.; Targovnik, Hector Manuel; Genomic organization of the 5′ region of the human thyroglobulin gene; BioScientifica; European Journal of Endocrinology; 143; 6; 12-2000; 789-7980804-4643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/eje.0.1430789info:eu-repo/semantics/altIdentifier/url/http://www.eje-online.org/content/143/6/789info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:35Zoai:ri.conicet.gov.ar:11336/40328instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:36.114CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic organization of the 5′ region of the human thyroglobulin gene
title Genomic organization of the 5′ region of the human thyroglobulin gene
spellingShingle Genomic organization of the 5′ region of the human thyroglobulin gene
Moya, C. M.
Genomic Organization
Thyroglobulin Gene
5' Region
Exon/Intron
title_short Genomic organization of the 5′ region of the human thyroglobulin gene
title_full Genomic organization of the 5′ region of the human thyroglobulin gene
title_fullStr Genomic organization of the 5′ region of the human thyroglobulin gene
title_full_unstemmed Genomic organization of the 5′ region of the human thyroglobulin gene
title_sort Genomic organization of the 5′ region of the human thyroglobulin gene
dc.creator.none.fl_str_mv Moya, C. M.
Mendive, F. M.
Rivolta, Carina Marcela
Vassart, G.
Targovnik, Hector Manuel
author Moya, C. M.
author_facet Moya, C. M.
Mendive, F. M.
Rivolta, Carina Marcela
Vassart, G.
Targovnik, Hector Manuel
author_role author
author2 Mendive, F. M.
Rivolta, Carina Marcela
Vassart, G.
Targovnik, Hector Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Genomic Organization
Thyroglobulin Gene
5' Region
Exon/Intron
topic Genomic Organization
Thyroglobulin Gene
5' Region
Exon/Intron
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: The purpose of the present work is to establish the intron - exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5′ terminal half of the gene. Design: Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. Methods: A human genomic DNA library was screened by in situ hybridization. Southern blotting experiments were performed to characterize the phage inserts. Intron/exon junction sequences were determined by the Taq polymerase-based chain terminator method. Finally, the thyroglobulin gene was mapped using the Gene Bridge 4 radiation hybrid clone panel. Results: We isolated and characterized four λ phage clones that include nucleotides 3002 to 4816 of the thyroglobulin mRNA, encompassing exons 12 to 23 of the gene. The exon sizes range between 78 and 219 nucleotides. We found that the GT-AG splicing sequences rule was perfectly respected in all the introns. A total of 7302 intronic bases was analyzed. Hormogenic tyrosine 5 and 1291 are encoded by exons 2 and 18. Also, seven alternative spliced variants are associated with the 5′ region. Thyroglobulin gene maps to 5.5 centiRays from the AFMAO53XF1 marker, in chromosome 8. Conclusions: The present study shows that the first 4857 bases of thyroglobulin mRNA are divided into 23 exons and the four phages isolated include 32.6 kb genomic DNA, covering 1815 nucleotides of exonic sequence distributed in 12 exons, from exon 12 to 23.
Fil: Moya, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mendive, F. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Rivolta, Carina Marcela. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vassart, G.. Université Libre de Bruxelles; Bélgica
Fil: Targovnik, Hector Manuel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Université Libre de Bruxelles; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: The purpose of the present work is to establish the intron - exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5′ terminal half of the gene. Design: Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. Methods: A human genomic DNA library was screened by in situ hybridization. Southern blotting experiments were performed to characterize the phage inserts. Intron/exon junction sequences were determined by the Taq polymerase-based chain terminator method. Finally, the thyroglobulin gene was mapped using the Gene Bridge 4 radiation hybrid clone panel. Results: We isolated and characterized four λ phage clones that include nucleotides 3002 to 4816 of the thyroglobulin mRNA, encompassing exons 12 to 23 of the gene. The exon sizes range between 78 and 219 nucleotides. We found that the GT-AG splicing sequences rule was perfectly respected in all the introns. A total of 7302 intronic bases was analyzed. Hormogenic tyrosine 5 and 1291 are encoded by exons 2 and 18. Also, seven alternative spliced variants are associated with the 5′ region. Thyroglobulin gene maps to 5.5 centiRays from the AFMAO53XF1 marker, in chromosome 8. Conclusions: The present study shows that the first 4857 bases of thyroglobulin mRNA are divided into 23 exons and the four phages isolated include 32.6 kb genomic DNA, covering 1815 nucleotides of exonic sequence distributed in 12 exons, from exon 12 to 23.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40328
Moya, C. M.; Mendive, F. M.; Rivolta, Carina Marcela; Vassart, G.; Targovnik, Hector Manuel; Genomic organization of the 5′ region of the human thyroglobulin gene; BioScientifica; European Journal of Endocrinology; 143; 6; 12-2000; 789-798
0804-4643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40328
identifier_str_mv Moya, C. M.; Mendive, F. M.; Rivolta, Carina Marcela; Vassart, G.; Targovnik, Hector Manuel; Genomic organization of the 5′ region of the human thyroglobulin gene; BioScientifica; European Journal of Endocrinology; 143; 6; 12-2000; 789-798
0804-4643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/eje.0.1430789
info:eu-repo/semantics/altIdentifier/url/http://www.eje-online.org/content/143/6/789
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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