Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz

Autores
Zufriategui, Camila; Fernandez, Patricia Carina; Rossini, Carmen; Porrini, Martín Pablo; Baldo, María Belén; Eguaras, Martin Javier; Garrido, Paula Melisa
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Cuticular hydrocarbons (CHC) are key-essential semiochemicals for honeybees with multiple functions, including the reduction of water loss and facilitation of intraspecific communication, such as recognition of nestmates. CHC patterns can be modified by genotype, physiological state, pathogens, and environmental context. Therefore, the present study aimed to characterize the CHC profile of Apis mellifera worker bees exposed to two stressors frequently found within the colony: Nosema ceranae - a prevalent fungal parasite- , and amitraz-an acaricide widely used for treating Varroa disease-. A semi-field assay was conducted using eight healthy hives, half of which were treated with amitraz strips (a drug commonly used to control mite disease). Three day-old-bees were individually marked on the thorax and introduced into the hives. Half of these introduced bees were infected with an inoculum of ca. 100000 N. ceranae spores. Consequently, four treatment groups were established: a) Control, b) Exposed to amitraz, c) Infected with N. ceranae spores, d) Infected with N. ceranae and exposed to amitraz. Afterwards, bees were sampled at two monitoring points (10 and 18-days post-emergence), and both parasitic load and CHC were quantified. When analyzing the evolution of the total CHC, it revealed a general reduction in the total mass for all treatments, except for the worker bees exposed to amitraz, whose values remained similar over time. However, focusing on the analysis of the 14 identified major CHC peaks, it was possible to recognize five hydrocarbons (nonacosane, ?-hentriacontene,?-hentriacontene, hentricontane, and tritriacontadiene) that exhibited a significant reduction in bees exposed to each one of the stress factors or their combination. Our results evidence a potential impact of chemical and biological stressors on in-hive communication cues. Whether these changes in chemical signaling are sufficient to be associated with alterations of social interactions is discussed.
Fil: Zufriategui, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Fernandez, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Rossini, Carmen. Universidad de la República. Facultad de Química. Departamento de Química Orgánica. Laboratorio de Ecología Química; Uruguay
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Baldo, María Belén. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
VII Congress of the Latin American Association of Chemical Ecology
Buenos Aires
Argentina
Universidad de Buenos Aires
Latin American Association of Chemical Ecology
Materia
Apis mellifera
Nosema ceranae
Cuticular hydrocarbons
Stressors
Honey bees
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253257
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/253257
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitrazZufriategui, CamilaFernandez, Patricia CarinaRossini, CarmenPorrini, Martín PabloBaldo, María BelénEguaras, Martin JavierGarrido, Paula MelisaApis melliferaNosema ceranaeCuticular hydrocarbonsStressorsHoney beeshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Cuticular hydrocarbons (CHC) are key-essential semiochemicals for honeybees with multiple functions, including the reduction of water loss and facilitation of intraspecific communication, such as recognition of nestmates. CHC patterns can be modified by genotype, physiological state, pathogens, and environmental context. Therefore, the present study aimed to characterize the CHC profile of Apis mellifera worker bees exposed to two stressors frequently found within the colony: Nosema ceranae - a prevalent fungal parasite- , and amitraz-an acaricide widely used for treating Varroa disease-. A semi-field assay was conducted using eight healthy hives, half of which were treated with amitraz strips (a drug commonly used to control mite disease). Three day-old-bees were individually marked on the thorax and introduced into the hives. Half of these introduced bees were infected with an inoculum of ca. 100000 N. ceranae spores. Consequently, four treatment groups were established: a) Control, b) Exposed to amitraz, c) Infected with N. ceranae spores, d) Infected with N. ceranae and exposed to amitraz. Afterwards, bees were sampled at two monitoring points (10 and 18-days post-emergence), and both parasitic load and CHC were quantified. When analyzing the evolution of the total CHC, it revealed a general reduction in the total mass for all treatments, except for the worker bees exposed to amitraz, whose values remained similar over time. However, focusing on the analysis of the 14 identified major CHC peaks, it was possible to recognize five hydrocarbons (nonacosane, ?-hentriacontene,?-hentriacontene, hentricontane, and tritriacontadiene) that exhibited a significant reduction in bees exposed to each one of the stress factors or their combination. Our results evidence a potential impact of chemical and biological stressors on in-hive communication cues. Whether these changes in chemical signaling are sufficient to be associated with alterations of social interactions is discussed.Fil: Zufriategui, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Fernandez, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Rossini, Carmen. Universidad de la República. Facultad de Química. Departamento de Química Orgánica. Laboratorio de Ecología Química; UruguayFil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Baldo, María Belén. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaVII Congress of the Latin American Association of Chemical EcologyBuenos AiresArgentinaUniversidad de Buenos AiresLatin American Association of Chemical EcologyUniversidad de Buenos Aires2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253257Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz; VII Congress of the Latin American Association of Chemical Ecology; Buenos Aires; Argentina; 2023; 204-204CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.alaeq.org/vii-congress-of-the-latin-american-association-of-chemical-ecology/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:37Zoai:ri.conicet.gov.ar:11336/253257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:37.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
title Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
spellingShingle Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
Zufriategui, Camila
Apis mellifera
Nosema ceranae
Cuticular hydrocarbons
Stressors
Honey bees
title_short Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
title_full Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
title_fullStr Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
title_full_unstemmed Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
title_sort Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz
dc.creator.none.fl_str_mv Zufriategui, Camila
Fernandez, Patricia Carina
Rossini, Carmen
Porrini, Martín Pablo
Baldo, María Belén
Eguaras, Martin Javier
Garrido, Paula Melisa
author Zufriategui, Camila
author_facet Zufriategui, Camila
Fernandez, Patricia Carina
Rossini, Carmen
Porrini, Martín Pablo
Baldo, María Belén
Eguaras, Martin Javier
Garrido, Paula Melisa
author_role author
author2 Fernandez, Patricia Carina
Rossini, Carmen
Porrini, Martín Pablo
Baldo, María Belén
Eguaras, Martin Javier
Garrido, Paula Melisa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apis mellifera
Nosema ceranae
Cuticular hydrocarbons
Stressors
Honey bees
topic Apis mellifera
Nosema ceranae
Cuticular hydrocarbons
Stressors
Honey bees
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cuticular hydrocarbons (CHC) are key-essential semiochemicals for honeybees with multiple functions, including the reduction of water loss and facilitation of intraspecific communication, such as recognition of nestmates. CHC patterns can be modified by genotype, physiological state, pathogens, and environmental context. Therefore, the present study aimed to characterize the CHC profile of Apis mellifera worker bees exposed to two stressors frequently found within the colony: Nosema ceranae - a prevalent fungal parasite- , and amitraz-an acaricide widely used for treating Varroa disease-. A semi-field assay was conducted using eight healthy hives, half of which were treated with amitraz strips (a drug commonly used to control mite disease). Three day-old-bees were individually marked on the thorax and introduced into the hives. Half of these introduced bees were infected with an inoculum of ca. 100000 N. ceranae spores. Consequently, four treatment groups were established: a) Control, b) Exposed to amitraz, c) Infected with N. ceranae spores, d) Infected with N. ceranae and exposed to amitraz. Afterwards, bees were sampled at two monitoring points (10 and 18-days post-emergence), and both parasitic load and CHC were quantified. When analyzing the evolution of the total CHC, it revealed a general reduction in the total mass for all treatments, except for the worker bees exposed to amitraz, whose values remained similar over time. However, focusing on the analysis of the 14 identified major CHC peaks, it was possible to recognize five hydrocarbons (nonacosane, ?-hentriacontene,?-hentriacontene, hentricontane, and tritriacontadiene) that exhibited a significant reduction in bees exposed to each one of the stress factors or their combination. Our results evidence a potential impact of chemical and biological stressors on in-hive communication cues. Whether these changes in chemical signaling are sufficient to be associated with alterations of social interactions is discussed.
Fil: Zufriategui, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Fernandez, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Rossini, Carmen. Universidad de la República. Facultad de Química. Departamento de Química Orgánica. Laboratorio de Ecología Química; Uruguay
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Baldo, María Belén. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
VII Congress of the Latin American Association of Chemical Ecology
Buenos Aires
Argentina
Universidad de Buenos Aires
Latin American Association of Chemical Ecology
description Cuticular hydrocarbons (CHC) are key-essential semiochemicals for honeybees with multiple functions, including the reduction of water loss and facilitation of intraspecific communication, such as recognition of nestmates. CHC patterns can be modified by genotype, physiological state, pathogens, and environmental context. Therefore, the present study aimed to characterize the CHC profile of Apis mellifera worker bees exposed to two stressors frequently found within the colony: Nosema ceranae - a prevalent fungal parasite- , and amitraz-an acaricide widely used for treating Varroa disease-. A semi-field assay was conducted using eight healthy hives, half of which were treated with amitraz strips (a drug commonly used to control mite disease). Three day-old-bees were individually marked on the thorax and introduced into the hives. Half of these introduced bees were infected with an inoculum of ca. 100000 N. ceranae spores. Consequently, four treatment groups were established: a) Control, b) Exposed to amitraz, c) Infected with N. ceranae spores, d) Infected with N. ceranae and exposed to amitraz. Afterwards, bees were sampled at two monitoring points (10 and 18-days post-emergence), and both parasitic load and CHC were quantified. When analyzing the evolution of the total CHC, it revealed a general reduction in the total mass for all treatments, except for the worker bees exposed to amitraz, whose values remained similar over time. However, focusing on the analysis of the 14 identified major CHC peaks, it was possible to recognize five hydrocarbons (nonacosane, ?-hentriacontene,?-hentriacontene, hentricontane, and tritriacontadiene) that exhibited a significant reduction in bees exposed to each one of the stress factors or their combination. Our results evidence a potential impact of chemical and biological stressors on in-hive communication cues. Whether these changes in chemical signaling are sufficient to be associated with alterations of social interactions is discussed.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253257
Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz; VII Congress of the Latin American Association of Chemical Ecology; Buenos Aires; Argentina; 2023; 204-204
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253257
identifier_str_mv Cuticular hydrocarbon profiles of honey bees (Apis mellifera) change after parasitized by Nosema ceranae in hives treated with amitraz; VII Congress of the Latin American Association of Chemical Ecology; Buenos Aires; Argentina; 2023; 204-204
CONICET Digital
CONICET
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language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Buenos Aires
publisher.none.fl_str_mv Universidad de Buenos Aires
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