Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study

Autores
Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; Santagapita, Patricio Roman; Rocculi, Pietro
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response.
U ovom radu su prikazani rezultati poređenja različitih analitičkih tehnika (Diferencijalna skeniranja Kalorimetrija – DSC, Niska Polje nuklearna magnetna rezonanca - LF-NMR, svetlost mikroskopije - LM i Prenos elektronsku mikroskopiju - TEM), u cilju određivanja prenosa mase, statusa vode i modifikacije ćelija tkiva perikarpa kivija tokom osmotske dehidratacije tretmana (OD). Dve sorte kivija su bile izložene osmotskom tretmanu, A. deliciosa i A. chinensis. Osmotsko sušenje obavljeno je u 61,5% rastvoru saharoze na tri različite temperature (25, 35 i 45°C), sa vremenom trajanja sušenja od 0 do 300 minuta. Pelegovim modelom naglašene su glavne razlike između dve sorte kivija koje se događaju tokom početne faze osmotskog tretmana. DSC parametri osetljivi su na izmenu vode i čvrste materije između voća i osmotskog rastvora. LF-NMR-om otkrivene su posledice razmene vode i čvrste materije na ćeliju, odnosno vakuole, citoplazmu sa vanćelijskim prostorom i ćelijski zid. Tokom OD, smanjenje vakuole protona bazena, otkrivena je LF-NMR, predložio skupljanja takvog odeljka, potvrđeno LM. Ćelijske zidove spoljne perikarpa su pokazali značajne promene u veličini, strukturi i mrlja uzimanja u toku OD posmatrano na sistem. Predloženi multianalitical pristupi treba da omogući bolji dizajn u kombinaciji obrade tehnologija dozvoljava procenu njihovog uticaja na tkivo odgovor.
Fil: Dalla Rosa, Marco. Università di Bologna; Italia
Fil: Tylewicz, Urszula. Università di Bologna; Italia
Fil: Panarese, Valentina. Università di Bologna; Italia
Fil: Laghi, Luca. Università di Bologna; Italia
Fil: Pisi, Annamaria. Università di Bologna; Italia
Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rocculi, Pietro. Università di Bologna; Italia
Materia
osmotic dehydration
cell compartments
DSC
NMR
TEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115297

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oai_identifier_str oai:ri.conicet.gov.ar:11336/115297
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network_name_str CONICET Digital (CONICET)
spelling Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural studyUticaj osmotske dehidracije na kivi: rezultati multianalitičkog pristupa proučavanja struktureDalla Rosa, MarcoTylewicz, UrszulaPanarese, ValentinaLaghi, LucaPisi, AnnamariaSantagapita, Patricio RomanRocculi, Pietroosmotic dehydrationcell compartmentsDSCNMRTEMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response.U ovom radu su prikazani rezultati poređenja različitih analitičkih tehnika (Diferencijalna skeniranja Kalorimetrija – DSC, Niska Polje nuklearna magnetna rezonanca - LF-NMR, svetlost mikroskopije - LM i Prenos elektronsku mikroskopiju - TEM), u cilju određivanja prenosa mase, statusa vode i modifikacije ćelija tkiva perikarpa kivija tokom osmotske dehidratacije tretmana (OD). Dve sorte kivija su bile izložene osmotskom tretmanu, A. deliciosa i A. chinensis. Osmotsko sušenje obavljeno je u 61,5% rastvoru saharoze na tri različite temperature (25, 35 i 45°C), sa vremenom trajanja sušenja od 0 do 300 minuta. Pelegovim modelom naglašene su glavne razlike između dve sorte kivija koje se događaju tokom početne faze osmotskog tretmana. DSC parametri osetljivi su na izmenu vode i čvrste materije između voća i osmotskog rastvora. LF-NMR-om otkrivene su posledice razmene vode i čvrste materije na ćeliju, odnosno vakuole, citoplazmu sa vanćelijskim prostorom i ćelijski zid. Tokom OD, smanjenje vakuole protona bazena, otkrivena je LF-NMR, predložio skupljanja takvog odeljka, potvrđeno LM. Ćelijske zidove spoljne perikarpa su pokazali značajne promene u veličini, strukturi i mrlja uzimanja u toku OD posmatrano na sistem. Predloženi multianalitical pristupi treba da omogući bolji dizajn u kombinaciji obrade tehnologija dozvoljava procenu njihovog uticaja na tkivo odgovor.Fil: Dalla Rosa, Marco. Università di Bologna; ItaliaFil: Tylewicz, Urszula. Università di Bologna; ItaliaFil: Panarese, Valentina. Università di Bologna; ItaliaFil: Laghi, Luca. Università di Bologna; ItaliaFil: Pisi, Annamaria. Università di Bologna; ItaliaFil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rocculi, Pietro. Università di Bologna; ItaliaNational Society of Processing and Energy in Agriculture2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115297Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; et al.; Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study; National Society of Processing and Energy in Agriculture; Journal on Processing and Energy in Agriculture; 15; 3; 10-2011; 113-1171821-4487CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scindeks-clanci.ceon.rs/data/pdf/1821-4487/2011/1821-44871103113R.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:48Zoai:ri.conicet.gov.ar:11336/115297instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:49.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
Uticaj osmotske dehidracije na kivi: rezultati multianalitičkog pristupa proučavanja strukture
title Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
spellingShingle Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
Dalla Rosa, Marco
osmotic dehydration
cell compartments
DSC
NMR
TEM
title_short Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
title_full Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
title_fullStr Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
title_full_unstemmed Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
title_sort Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study
dc.creator.none.fl_str_mv Dalla Rosa, Marco
Tylewicz, Urszula
Panarese, Valentina
Laghi, Luca
Pisi, Annamaria
Santagapita, Patricio Roman
Rocculi, Pietro
author Dalla Rosa, Marco
author_facet Dalla Rosa, Marco
Tylewicz, Urszula
Panarese, Valentina
Laghi, Luca
Pisi, Annamaria
Santagapita, Patricio Roman
Rocculi, Pietro
author_role author
author2 Tylewicz, Urszula
Panarese, Valentina
Laghi, Luca
Pisi, Annamaria
Santagapita, Patricio Roman
Rocculi, Pietro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv osmotic dehydration
cell compartments
DSC
NMR
TEM
topic osmotic dehydration
cell compartments
DSC
NMR
TEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response.
U ovom radu su prikazani rezultati poređenja različitih analitičkih tehnika (Diferencijalna skeniranja Kalorimetrija – DSC, Niska Polje nuklearna magnetna rezonanca - LF-NMR, svetlost mikroskopije - LM i Prenos elektronsku mikroskopiju - TEM), u cilju određivanja prenosa mase, statusa vode i modifikacije ćelija tkiva perikarpa kivija tokom osmotske dehidratacije tretmana (OD). Dve sorte kivija su bile izložene osmotskom tretmanu, A. deliciosa i A. chinensis. Osmotsko sušenje obavljeno je u 61,5% rastvoru saharoze na tri različite temperature (25, 35 i 45°C), sa vremenom trajanja sušenja od 0 do 300 minuta. Pelegovim modelom naglašene su glavne razlike između dve sorte kivija koje se događaju tokom početne faze osmotskog tretmana. DSC parametri osetljivi su na izmenu vode i čvrste materije između voća i osmotskog rastvora. LF-NMR-om otkrivene su posledice razmene vode i čvrste materije na ćeliju, odnosno vakuole, citoplazmu sa vanćelijskim prostorom i ćelijski zid. Tokom OD, smanjenje vakuole protona bazena, otkrivena je LF-NMR, predložio skupljanja takvog odeljka, potvrđeno LM. Ćelijske zidove spoljne perikarpa su pokazali značajne promene u veličini, strukturi i mrlja uzimanja u toku OD posmatrano na sistem. Predloženi multianalitical pristupi treba da omogući bolji dizajn u kombinaciji obrade tehnologija dozvoljava procenu njihovog uticaja na tkivo odgovor.
Fil: Dalla Rosa, Marco. Università di Bologna; Italia
Fil: Tylewicz, Urszula. Università di Bologna; Italia
Fil: Panarese, Valentina. Università di Bologna; Italia
Fil: Laghi, Luca. Università di Bologna; Italia
Fil: Pisi, Annamaria. Università di Bologna; Italia
Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rocculi, Pietro. Università di Bologna; Italia
description This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115297
Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; et al.; Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study; National Society of Processing and Energy in Agriculture; Journal on Processing and Energy in Agriculture; 15; 3; 10-2011; 113-117
1821-4487
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115297
identifier_str_mv Dalla Rosa, Marco; Tylewicz, Urszula; Panarese, Valentina; Laghi, Luca; Pisi, Annamaria; et al.; Effect of osmotic dehydration on kiwifruit: Results of a multianalytical approach to structural study; National Society of Processing and Energy in Agriculture; Journal on Processing and Energy in Agriculture; 15; 3; 10-2011; 113-117
1821-4487
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scindeks-clanci.ceon.rs/data/pdf/1821-4487/2011/1821-44871103113R.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Society of Processing and Energy in Agriculture
publisher.none.fl_str_mv National Society of Processing and Energy in Agriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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