Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles

Autores
Moredo, Fabiana A.; Piñeyro Piñeiro, Pablo Enrique; Márquez, Gabriela C.; Sanz, Marcelo; Colello, Rocío; Etcheverría, Analía Inés; Padola, Nora L.; Quiroga, Maria A.; Perfumo, Carlos J.; Galli, Lucía; Leotta, Gerardo Anibal
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen’s presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfbO157/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx2e/estI, stx2e/estI/estII, and stx2e/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus–PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.
Fil: Moredo, Fabiana A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Microbiologia. Catedra de Microbiologia; Argentina
Fil: Piñeyro Piñeiro, Pablo Enrique. University of Iowa; Estados Unidos
Fil: Márquez, Gabriela C.. Virginia Polytechnic Institute; Estados Unidos
Fil: Sanz, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Padola, Nora L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Quiroga, Maria A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; Argentina
Fil: Perfumo, Carlos J.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Etec
Pigs
Pcr
Eric
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11798
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11798
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network_name_str CONICET Digital (CONICET)
spelling Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profilesMoredo, Fabiana A.Piñeyro Piñeiro, Pablo EnriqueMárquez, Gabriela C.Sanz, MarceloColello, RocíoEtcheverría, Analía InésPadola, Nora L.Quiroga, Maria A.Perfumo, Carlos J.Galli, LucíaLeotta, Gerardo AnibalEtecPigsPcrErichttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen’s presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfbO157/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx2e/estI, stx2e/estI/estII, and stx2e/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus–PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.Fil: Moredo, Fabiana A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Microbiologia. Catedra de Microbiologia; ArgentinaFil: Piñeyro Piñeiro, Pablo Enrique. University of Iowa; Estados UnidosFil: Márquez, Gabriela C.. Virginia Polytechnic Institute; Estados UnidosFil: Sanz, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Padola, Nora L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Quiroga, Maria A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; ArgentinaFil: Perfumo, Carlos J.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaMary Ann Liebert Inc2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11798Moredo, Fabiana A.; Piñeyro Piñeiro, Pablo Enrique; Márquez, Gabriela C.; Sanz, Marcelo; Colello, Rocío; et al.; Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 12; 8; 8-2015; 704-7111535-31411556-7125enginfo:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2015.1959info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/10.1089/fpd.2015.1959info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:26Zoai:ri.conicet.gov.ar:11336/11798instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:26.25CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
title Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
spellingShingle Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
Moredo, Fabiana A.
Etec
Pigs
Pcr
Eric
title_short Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
title_full Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
title_fullStr Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
title_full_unstemmed Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
title_sort Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles
dc.creator.none.fl_str_mv Moredo, Fabiana A.
Piñeyro Piñeiro, Pablo Enrique
Márquez, Gabriela C.
Sanz, Marcelo
Colello, Rocío
Etcheverría, Analía Inés
Padola, Nora L.
Quiroga, Maria A.
Perfumo, Carlos J.
Galli, Lucía
Leotta, Gerardo Anibal
author Moredo, Fabiana A.
author_facet Moredo, Fabiana A.
Piñeyro Piñeiro, Pablo Enrique
Márquez, Gabriela C.
Sanz, Marcelo
Colello, Rocío
Etcheverría, Analía Inés
Padola, Nora L.
Quiroga, Maria A.
Perfumo, Carlos J.
Galli, Lucía
Leotta, Gerardo Anibal
author_role author
author2 Piñeyro Piñeiro, Pablo Enrique
Márquez, Gabriela C.
Sanz, Marcelo
Colello, Rocío
Etcheverría, Analía Inés
Padola, Nora L.
Quiroga, Maria A.
Perfumo, Carlos J.
Galli, Lucía
Leotta, Gerardo Anibal
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Etec
Pigs
Pcr
Eric
topic Etec
Pigs
Pcr
Eric
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen’s presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfbO157/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx2e/estI, stx2e/estI/estII, and stx2e/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus–PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.
Fil: Moredo, Fabiana A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Microbiologia. Catedra de Microbiologia; Argentina
Fil: Piñeyro Piñeiro, Pablo Enrique. University of Iowa; Estados Unidos
Fil: Márquez, Gabriela C.. Virginia Polytechnic Institute; Estados Unidos
Fil: Sanz, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Padola, Nora L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Quiroga, Maria A.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; Argentina
Fil: Perfumo, Carlos J.. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Patologia. Laboratorio de Patolog. Espec. Veterinaria "dr. Bernardo Epstein"; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen’s presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfbO157/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx2e/estI, stx2e/estI/estII, and stx2e/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus–PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11798
Moredo, Fabiana A.; Piñeyro Piñeiro, Pablo Enrique; Márquez, Gabriela C.; Sanz, Marcelo; Colello, Rocío; et al.; Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 12; 8; 8-2015; 704-711
1535-3141
1556-7125
url http://hdl.handle.net/11336/11798
identifier_str_mv Moredo, Fabiana A.; Piñeyro Piñeiro, Pablo Enrique; Márquez, Gabriela C.; Sanz, Marcelo; Colello, Rocío; et al.; Enterotoxigenic Escherichia Coli subclinical infection in pigs: bacteriological and genotypic characterization and antimicrobial resistance profiles; Mary Ann Liebert Inc; Foodborne Pathogens And Disease; 12; 8; 8-2015; 704-711
1535-3141
1556-7125
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2015.1959
info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/10.1089/fpd.2015.1959
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc
publisher.none.fl_str_mv Mary Ann Liebert Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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