Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process

Autores
Vázquez, Cecilia Alejandra; Escudero Pérez, Beatriz; Hayashi, Jennifer M.; Leon, Kristoffer E.; Moreira, João Paulo; Castañeda Cataña, Mayra Alejandra; Groseth, Allison; Ott, Melanie; Oestereich, Lisa; Muñoz Fontela, César; Garcia, Cybele; Cordo, Sandra Myriam
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lipid droplets (LDs) are organelles involved in lipid storage, maintenance of energy homeostasis, protein sequestration, signaling events and inter-organelle interactions. Recently, LDs have been shown to favor the replication of members from different viral families, such as the Flaviviridae and Coronaviridae. In this work, we show that LDs are essential organelles for members of the Arenaviridae family. A virus-driven reduction of LD number was observed in cultures infected with Junín mammarenavirus (JUNV), caused in part by action of the viral nucleoprotein. Notably, we identified a new pool of nucleoprotein and viral RNA that localizes in the vicinity of LDs, suggesting that LDs play a role during the viral replication cycle. Regarding the mechanism behind LD exhaustion, we found evidence that lipophagy is involved in LD degradation with the resulting fatty acids being substrates of fatty acid β-oxidation, which fuels viral multiplication. This work highlights the importance of LDs during the replication cycle of JUNV, contributing to the knowledge of the metabolic changes these mammarenaviruses cause in their hosts.
Fil: Vázquez, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Escudero Pérez, Beatriz. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; Alemania
Fil: Hayashi, Jennifer M.. J. David Gladstone Institutes; Estados Unidos
Fil: Leon, Kristoffer E.. J. David Gladstone Institutes; Estados Unidos
Fil: Moreira, João Paulo. J. David Gladstone Institutes; Estados Unidos
Fil: Castañeda Cataña, Mayra Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Groseth, Allison. Friedrich-Loeffler-Institut; Alemania
Fil: Ott, Melanie. J. David Gladstone Institutes; Estados Unidos
Fil: Oestereich, Lisa. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; Alemania
Fil: Muñoz Fontela, César. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cordo, Sandra Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
JUNV
LIPIDS
LDs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268459
Acceder
id CONICETDig_6b35eedd7a3659172590275ba7d71dfb
oai_identifier_str oai:ri.conicet.gov.ar:11336/268459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent processVázquez, Cecilia AlejandraEscudero Pérez, BeatrizHayashi, Jennifer M.Leon, Kristoffer E.Moreira, João PauloCastañeda Cataña, Mayra AlejandraGroseth, AllisonOtt, MelanieOestereich, LisaMuñoz Fontela, CésarGarcia, CybeleCordo, Sandra MyriamJUNVLIPIDSLDshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lipid droplets (LDs) are organelles involved in lipid storage, maintenance of energy homeostasis, protein sequestration, signaling events and inter-organelle interactions. Recently, LDs have been shown to favor the replication of members from different viral families, such as the Flaviviridae and Coronaviridae. In this work, we show that LDs are essential organelles for members of the Arenaviridae family. A virus-driven reduction of LD number was observed in cultures infected with Junín mammarenavirus (JUNV), caused in part by action of the viral nucleoprotein. Notably, we identified a new pool of nucleoprotein and viral RNA that localizes in the vicinity of LDs, suggesting that LDs play a role during the viral replication cycle. Regarding the mechanism behind LD exhaustion, we found evidence that lipophagy is involved in LD degradation with the resulting fatty acids being substrates of fatty acid β-oxidation, which fuels viral multiplication. This work highlights the importance of LDs during the replication cycle of JUNV, contributing to the knowledge of the metabolic changes these mammarenaviruses cause in their hosts.Fil: Vázquez, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Escudero Pérez, Beatriz. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; AlemaniaFil: Hayashi, Jennifer M.. J. David Gladstone Institutes; Estados UnidosFil: Leon, Kristoffer E.. J. David Gladstone Institutes; Estados UnidosFil: Moreira, João Paulo. J. David Gladstone Institutes; Estados UnidosFil: Castañeda Cataña, Mayra Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Groseth, Allison. Friedrich-Loeffler-Institut; AlemaniaFil: Ott, Melanie. J. David Gladstone Institutes; Estados UnidosFil: Oestereich, Lisa. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; AlemaniaFil: Muñoz Fontela, César. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Cordo, Sandra Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaCompany of Biologists2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268459Vázquez, Cecilia Alejandra; Escudero Pérez, Beatriz; Hayashi, Jennifer M.; Leon, Kristoffer E.; Moreira, João Paulo; et al.; Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process; Company of Biologists; Journal of Cell Science; 137; 19; 10-2024; 1-130021-9533CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jcs/article/137/19/jcs261745/362351/Intracellular-lipid-droplets-are-exploited-byinfo:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.261745info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:52Zoai:ri.conicet.gov.ar:11336/268459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:52.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
title Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
spellingShingle Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
Vázquez, Cecilia Alejandra
JUNV
LIPIDS
LDs
title_short Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
title_full Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
title_fullStr Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
title_full_unstemmed Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
title_sort Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process
dc.creator.none.fl_str_mv Vázquez, Cecilia Alejandra
Escudero Pérez, Beatriz
Hayashi, Jennifer M.
Leon, Kristoffer E.
Moreira, João Paulo
Castañeda Cataña, Mayra Alejandra
Groseth, Allison
Ott, Melanie
Oestereich, Lisa
Muñoz Fontela, César
Garcia, Cybele
Cordo, Sandra Myriam
author Vázquez, Cecilia Alejandra
author_facet Vázquez, Cecilia Alejandra
Escudero Pérez, Beatriz
Hayashi, Jennifer M.
Leon, Kristoffer E.
Moreira, João Paulo
Castañeda Cataña, Mayra Alejandra
Groseth, Allison
Ott, Melanie
Oestereich, Lisa
Muñoz Fontela, César
Garcia, Cybele
Cordo, Sandra Myriam
author_role author
author2 Escudero Pérez, Beatriz
Hayashi, Jennifer M.
Leon, Kristoffer E.
Moreira, João Paulo
Castañeda Cataña, Mayra Alejandra
Groseth, Allison
Ott, Melanie
Oestereich, Lisa
Muñoz Fontela, César
Garcia, Cybele
Cordo, Sandra Myriam
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv JUNV
LIPIDS
LDs
topic JUNV
LIPIDS
LDs
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lipid droplets (LDs) are organelles involved in lipid storage, maintenance of energy homeostasis, protein sequestration, signaling events and inter-organelle interactions. Recently, LDs have been shown to favor the replication of members from different viral families, such as the Flaviviridae and Coronaviridae. In this work, we show that LDs are essential organelles for members of the Arenaviridae family. A virus-driven reduction of LD number was observed in cultures infected with Junín mammarenavirus (JUNV), caused in part by action of the viral nucleoprotein. Notably, we identified a new pool of nucleoprotein and viral RNA that localizes in the vicinity of LDs, suggesting that LDs play a role during the viral replication cycle. Regarding the mechanism behind LD exhaustion, we found evidence that lipophagy is involved in LD degradation with the resulting fatty acids being substrates of fatty acid β-oxidation, which fuels viral multiplication. This work highlights the importance of LDs during the replication cycle of JUNV, contributing to the knowledge of the metabolic changes these mammarenaviruses cause in their hosts.
Fil: Vázquez, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Escudero Pérez, Beatriz. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; Alemania
Fil: Hayashi, Jennifer M.. J. David Gladstone Institutes; Estados Unidos
Fil: Leon, Kristoffer E.. J. David Gladstone Institutes; Estados Unidos
Fil: Moreira, João Paulo. J. David Gladstone Institutes; Estados Unidos
Fil: Castañeda Cataña, Mayra Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Groseth, Allison. Friedrich-Loeffler-Institut; Alemania
Fil: Ott, Melanie. J. David Gladstone Institutes; Estados Unidos
Fil: Oestereich, Lisa. Bernhard Nocht Institute For Tropical Medicine.; Alemania. German Center for Infection Research; Alemania
Fil: Muñoz Fontela, César. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cordo, Sandra Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Lipid droplets (LDs) are organelles involved in lipid storage, maintenance of energy homeostasis, protein sequestration, signaling events and inter-organelle interactions. Recently, LDs have been shown to favor the replication of members from different viral families, such as the Flaviviridae and Coronaviridae. In this work, we show that LDs are essential organelles for members of the Arenaviridae family. A virus-driven reduction of LD number was observed in cultures infected with Junín mammarenavirus (JUNV), caused in part by action of the viral nucleoprotein. Notably, we identified a new pool of nucleoprotein and viral RNA that localizes in the vicinity of LDs, suggesting that LDs play a role during the viral replication cycle. Regarding the mechanism behind LD exhaustion, we found evidence that lipophagy is involved in LD degradation with the resulting fatty acids being substrates of fatty acid β-oxidation, which fuels viral multiplication. This work highlights the importance of LDs during the replication cycle of JUNV, contributing to the knowledge of the metabolic changes these mammarenaviruses cause in their hosts.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268459
Vázquez, Cecilia Alejandra; Escudero Pérez, Beatriz; Hayashi, Jennifer M.; Leon, Kristoffer E.; Moreira, João Paulo; et al.; Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process; Company of Biologists; Journal of Cell Science; 137; 19; 10-2024; 1-13
0021-9533
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268459
identifier_str_mv Vázquez, Cecilia Alejandra; Escudero Pérez, Beatriz; Hayashi, Jennifer M.; Leon, Kristoffer E.; Moreira, João Paulo; et al.; Intracellular lipid droplets are exploited by Junín virus in a nucleoprotein-dependent process; Company of Biologists; Journal of Cell Science; 137; 19; 10-2024; 1-13
0021-9533
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jcs/article/137/19/jcs261745/362351/Intracellular-lipid-droplets-are-exploited-by
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.261745
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083148408946688
score 13.22299

Publicaciones similares