Toxicological assessment of three medicinal plants from the Northwest of Argentina
- Autores
- Carabajal, Monica Patricia Antonella; Nicolella, H. D.; Squarisi, I.; Guissoni, A. P. P.; Zampini, Iris Catiana; Tavares, D. C.; Isla, Maria Ines
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.
Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina
Fil: Nicolella, H. D.. Universidade de Franca; Brasil
Fil: Squarisi, I.. Universidade de Franca; Brasil
Fil: Guissoni, A. P. P.. Universidade de Franca; Brasil
Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Tavares, D. C.. Universidade de Franca; Brasil
Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology
Vitoria
Brasil
Federal University of São Carlos
Federal University of Espírito Santo
University of São Paulo - Materia
-
Medicinal plants
Toxicological analysis
Jarillas - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/238714
Ver los metadatos del registro completo
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Toxicological assessment of three medicinal plants from the Northwest of ArgentinaCarabajal, Monica Patricia AntonellaNicolella, H. D.Squarisi, I.Guissoni, A. P. P.Zampini, Iris CatianaTavares, D. C.Isla, Maria InesMedicinal plantsToxicological analysisJarillashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Nicolella, H. D.. Universidade de Franca; BrasilFil: Squarisi, I.. Universidade de Franca; BrasilFil: Guissoni, A. P. P.. Universidade de Franca; BrasilFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Tavares, D. C.. Universidade de Franca; BrasilFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and EcologyVitoriaBrasilFederal University of São CarlosFederal University of Espírito SantoUniversity of São PauloUniversidade Federal do Espirito Santo2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238714Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://proceedings.science/bcnp-series/bcnp/papers/toxicological-assessment-of-three-medicinal-plants-from-the-northwest-of-argenti?lang=enInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:17Zoai:ri.conicet.gov.ar:11336/238714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:17.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
title |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
spellingShingle |
Toxicological assessment of three medicinal plants from the Northwest of Argentina Carabajal, Monica Patricia Antonella Medicinal plants Toxicological analysis Jarillas |
title_short |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
title_full |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
title_fullStr |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
title_full_unstemmed |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
title_sort |
Toxicological assessment of three medicinal plants from the Northwest of Argentina |
dc.creator.none.fl_str_mv |
Carabajal, Monica Patricia Antonella Nicolella, H. D. Squarisi, I. Guissoni, A. P. P. Zampini, Iris Catiana Tavares, D. C. Isla, Maria Ines |
author |
Carabajal, Monica Patricia Antonella |
author_facet |
Carabajal, Monica Patricia Antonella Nicolella, H. D. Squarisi, I. Guissoni, A. P. P. Zampini, Iris Catiana Tavares, D. C. Isla, Maria Ines |
author_role |
author |
author2 |
Nicolella, H. D. Squarisi, I. Guissoni, A. P. P. Zampini, Iris Catiana Tavares, D. C. Isla, Maria Ines |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Medicinal plants Toxicological analysis Jarillas |
topic |
Medicinal plants Toxicological analysis Jarillas |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species. Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Nicolella, H. D.. Universidade de Franca; Brasil Fil: Squarisi, I.. Universidade de Franca; Brasil Fil: Guissoni, A. P. P.. Universidade de Franca; Brasil Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Tavares, D. C.. Universidade de Franca; Brasil Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology Vitoria Brasil Federal University of São Carlos Federal University of Espírito Santo University of São Paulo |
description |
In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
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info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Conferencia Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
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conferenceObject |
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http://hdl.handle.net/11336/238714 Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/238714 |
identifier_str_mv |
Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1 CONICET Digital CONICET |
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eng |
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eng |
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Internacional |
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Universidade Federal do Espirito Santo |
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Universidade Federal do Espirito Santo |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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