Toxicological assessment of three medicinal plants from the Northwest of Argentina

Autores
Carabajal, Monica Patricia Antonella; Nicolella, H. D.; Squarisi, I.; Guissoni, A. P. P.; Zampini, Iris Catiana; Tavares, D. C.; Isla, Maria Ines
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.
Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina
Fil: Nicolella, H. D.. Universidade de Franca; Brasil
Fil: Squarisi, I.. Universidade de Franca; Brasil
Fil: Guissoni, A. P. P.. Universidade de Franca; Brasil
Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Tavares, D. C.. Universidade de Franca; Brasil
Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology
Vitoria
Brasil
Federal University of São Carlos
Federal University of Espírito Santo
University of São Paulo
Materia
Medicinal plants
Toxicological analysis
Jarillas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238714

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network_name_str CONICET Digital (CONICET)
spelling Toxicological assessment of three medicinal plants from the Northwest of ArgentinaCarabajal, Monica Patricia AntonellaNicolella, H. D.Squarisi, I.Guissoni, A. P. P.Zampini, Iris CatianaTavares, D. C.Isla, Maria InesMedicinal plantsToxicological analysisJarillashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Nicolella, H. D.. Universidade de Franca; BrasilFil: Squarisi, I.. Universidade de Franca; BrasilFil: Guissoni, A. P. P.. Universidade de Franca; BrasilFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Tavares, D. C.. Universidade de Franca; BrasilFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and EcologyVitoriaBrasilFederal University of São CarlosFederal University of Espírito SantoUniversity of São PauloUniversidade Federal do Espirito Santo2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238714Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://proceedings.science/bcnp-series/bcnp/papers/toxicological-assessment-of-three-medicinal-plants-from-the-northwest-of-argenti?lang=enInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:17Zoai:ri.conicet.gov.ar:11336/238714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:17.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxicological assessment of three medicinal plants from the Northwest of Argentina
title Toxicological assessment of three medicinal plants from the Northwest of Argentina
spellingShingle Toxicological assessment of three medicinal plants from the Northwest of Argentina
Carabajal, Monica Patricia Antonella
Medicinal plants
Toxicological analysis
Jarillas
title_short Toxicological assessment of three medicinal plants from the Northwest of Argentina
title_full Toxicological assessment of three medicinal plants from the Northwest of Argentina
title_fullStr Toxicological assessment of three medicinal plants from the Northwest of Argentina
title_full_unstemmed Toxicological assessment of three medicinal plants from the Northwest of Argentina
title_sort Toxicological assessment of three medicinal plants from the Northwest of Argentina
dc.creator.none.fl_str_mv Carabajal, Monica Patricia Antonella
Nicolella, H. D.
Squarisi, I.
Guissoni, A. P. P.
Zampini, Iris Catiana
Tavares, D. C.
Isla, Maria Ines
author Carabajal, Monica Patricia Antonella
author_facet Carabajal, Monica Patricia Antonella
Nicolella, H. D.
Squarisi, I.
Guissoni, A. P. P.
Zampini, Iris Catiana
Tavares, D. C.
Isla, Maria Ines
author_role author
author2 Nicolella, H. D.
Squarisi, I.
Guissoni, A. P. P.
Zampini, Iris Catiana
Tavares, D. C.
Isla, Maria Ines
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicinal plants
Toxicological analysis
Jarillas
topic Medicinal plants
Toxicological analysis
Jarillas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.
Fil: Carabajal, Monica Patricia Antonella. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina
Fil: Nicolella, H. D.. Universidade de Franca; Brasil
Fil: Squarisi, I.. Universidade de Franca; Brasil
Fil: Guissoni, A. P. P.. Universidade de Franca; Brasil
Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Tavares, D. C.. Universidade de Franca; Brasil
Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology
Vitoria
Brasil
Federal University of São Carlos
Federal University of Espírito Santo
University of São Paulo
description In Argentina, the practice of plants-based traditional healing is still deeply rooted in cultures of indigenous and rural communities as primary health care. Some xeric vegetal species from the phytogeographical Monte region have been principally used as medicinal resources by local communities, among them “jarilla” species stand out. Zuccagnia punctata Cav., Larrea cuneifolia Cav. and Larrea divaricata Cav are constituents of “jarillal” (typical plants community), and several biological activities in vitro and in vivo were demonstrated for them, such as antioxidant, antigenotoxic, antimicrobial, among others. Considering the medicinal use of these plant species, the present study aimed to evaluate the mutagenic and cytotoxic potential of jarilla species that grown in the northwest of Argentina. Due to the main way of consumption is as infusion, they were prepared by the usual domestic preparation technique. The evaluation of mutagenicity was performed using the Salmonella/Microsome assay, with and without metabolic activation, based on the plate-incorporation method. The Brine Shrimp lethality assay was used to test acute toxicity. For the evaluation of cytotoxicity, the XTT assay was used in chinese hamster ovary cells (CHO) and normal human lung fibroblasts cell (GM07492A). The results showed that none of the species revealed mutagenic and pro-mutagenic effect by the retromutation bacterial assay. Z. punctata showed “slight toxicity” in the Brine Shrimp lethality assay with a LC50 equal to 720 µg/mL. Additionally, this plant extract showed minor values of IC50 against normal cell cultures, when compared to the species of the genus Larrea Nevertheless, it is worth to mention that the IC50 values in most cases were superiors to those concentrations that it present biological activities, as have been previously described. These results provide a preliminary toxicological profile of these medicinal infusions, in order to take safety measures in their consumption. Further studies are needed to better understand the toxic effect of these species.
publishDate 2017
dc.date.none.fl_str_mv 2017
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http://purl.org/coar/resource_type/c_5794
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238714
Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238714
identifier_str_mv Toxicological assessment of three medicinal plants from the Northwest of Argentina; 6th Brazilian Conference on Natural Products; XXXII Annual Meeting on Micromolecular Evolution, Systematics and Ecology; Vitoria; Brasil; 2017; 1-1
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Universidade Federal do Espirito Santo
publisher.none.fl_str_mv Universidade Federal do Espirito Santo
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