Comparative androgenetic competence of various species and genotypes within the genus Pisum L.

Autores
Bermejo, Carolina Julieta; Guindon, María Fernanda; Palacios Martinez, Laura Tatiana; Cazzola, Federico; Gatti, Ileana; Cointry Peix, Enrique Luis
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genus Pisum was undertaken. We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild forms Pisum fulvum Sibth. & Sm. and Pisum sativum subsp. elatius (Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes of P. sativum, the botanical variety arvense regenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogénita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively). P. fulvum, P. sativum subsp. elatius and P. sativum subsp sativum var arvense were identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties.
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Guindon, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Palacios Martinez, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Cazzola, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Gatti, Ileana. Universidad Nacional de Rosario; Argentina
Fil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
ANDROGENIC RESPONSE
ANTHER CULTURE
PISUM
PLANT REGENERATION
WILD RELATIVES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163350
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163350
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative androgenetic competence of various species and genotypes within the genus Pisum L.Bermejo, Carolina JulietaGuindon, María FernandaPalacios Martinez, Laura TatianaCazzola, FedericoGatti, IleanaCointry Peix, Enrique LuisANDROGENIC RESPONSEANTHER CULTUREPISUMPLANT REGENERATIONWILD RELATIVEShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genus Pisum was undertaken. We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild forms Pisum fulvum Sibth. & Sm. and Pisum sativum subsp. elatius (Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes of P. sativum, the botanical variety arvense regenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogénita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively). P. fulvum, P. sativum subsp. elatius and P. sativum subsp sativum var arvense were identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties.Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Guindon, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Palacios Martinez, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Cazzola, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Gatti, Ileana. Universidad Nacional de Rosario; ArgentinaFil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaSpringer2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163350Bermejo, Carolina Julieta; Guindon, María Fernanda; Palacios Martinez, Laura Tatiana; Cazzola, Federico; Gatti, Ileana; et al.; Comparative androgenetic competence of various species and genotypes within the genus Pisum L.; Springer; Plant Cell, Tissue and Organ Culture; 143; 3; 9-2020; 487-4970167-6857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-020-01934-yinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-020-01934-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:23:50Zoai:ri.conicet.gov.ar:11336/163350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:23:50.746CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
title Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
spellingShingle Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
Bermejo, Carolina Julieta
ANDROGENIC RESPONSE
ANTHER CULTURE
PISUM
PLANT REGENERATION
WILD RELATIVES
title_short Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
title_full Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
title_fullStr Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
title_full_unstemmed Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
title_sort Comparative androgenetic competence of various species and genotypes within the genus Pisum L.
dc.creator.none.fl_str_mv Bermejo, Carolina Julieta
Guindon, María Fernanda
Palacios Martinez, Laura Tatiana
Cazzola, Federico
Gatti, Ileana
Cointry Peix, Enrique Luis
author Bermejo, Carolina Julieta
author_facet Bermejo, Carolina Julieta
Guindon, María Fernanda
Palacios Martinez, Laura Tatiana
Cazzola, Federico
Gatti, Ileana
Cointry Peix, Enrique Luis
author_role author
author2 Guindon, María Fernanda
Palacios Martinez, Laura Tatiana
Cazzola, Federico
Gatti, Ileana
Cointry Peix, Enrique Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANDROGENIC RESPONSE
ANTHER CULTURE
PISUM
PLANT REGENERATION
WILD RELATIVES
topic ANDROGENIC RESPONSE
ANTHER CULTURE
PISUM
PLANT REGENERATION
WILD RELATIVES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genus Pisum was undertaken. We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild forms Pisum fulvum Sibth. & Sm. and Pisum sativum subsp. elatius (Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes of P. sativum, the botanical variety arvense regenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogénita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively). P. fulvum, P. sativum subsp. elatius and P. sativum subsp sativum var arvense were identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties.
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Guindon, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Palacios Martinez, Laura Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Cazzola, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Gatti, Ileana. Universidad Nacional de Rosario; Argentina
Fil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genus Pisum was undertaken. We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild forms Pisum fulvum Sibth. & Sm. and Pisum sativum subsp. elatius (Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes of P. sativum, the botanical variety arvense regenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogénita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively). P. fulvum, P. sativum subsp. elatius and P. sativum subsp sativum var arvense were identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163350
Bermejo, Carolina Julieta; Guindon, María Fernanda; Palacios Martinez, Laura Tatiana; Cazzola, Federico; Gatti, Ileana; et al.; Comparative androgenetic competence of various species and genotypes within the genus Pisum L.; Springer; Plant Cell, Tissue and Organ Culture; 143; 3; 9-2020; 487-497
0167-6857
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163350
identifier_str_mv Bermejo, Carolina Julieta; Guindon, María Fernanda; Palacios Martinez, Laura Tatiana; Cazzola, Federico; Gatti, Ileana; et al.; Comparative androgenetic competence of various species and genotypes within the genus Pisum L.; Springer; Plant Cell, Tissue and Organ Culture; 143; 3; 9-2020; 487-497
0167-6857
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-020-01934-y
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-020-01934-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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