MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling

Autores
Musri, Melina Mara; Coll Bonfill, Núria; Maron, Bradley A.; Peinado, Victor Ivo; Wang, Rui Sheng; Altirriba, Jordi; Blanco, Isabel; Oldham, William M.; Tura Ceide, Olga; García Lucio, Jessica; de la Cruz Thea, Benjamín Isaías; Meister, Gunter; Loscalzo, Joseph; Barberà, Joan A.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR- 139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflowobstruction andPAintimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotypemeasured by both cell cycle analysis and wound-healing assay. Using luciferase assays, Western blot, and quantitative PCR, we confirmed that miR-197 targets the transcription factor E2F1. In PAs from patients with COPD, levels of E2F1 were increased as compared withNS. InPAs of patients with COPD, remodeling of the vesselwall is associated with downregulation of miR-197, which regulates SMC phenotype. The effect ofmiR-197 onPAsmight bemediated, at least in part, by the key proproliferative factor, E2F1.
Fil: Musri, Melina Mara. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Coll Bonfill, Núria. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: Maron, Bradley A.. Brigham And Women's Hospital; Estados Unidos
Fil: Peinado, Victor Ivo. Universidad de Barcelona; España. Biomedical Research Centre Network For Respiratory Diseases; España
Fil: Wang, Rui Sheng. Brigham And Women's Hospital; Estados Unidos
Fil: Altirriba, Jordi. Universidad de Ginebra; Suiza
Fil: Blanco, Isabel. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: Oldham, William M.. Brigham And Women's Hospital; Estados Unidos
Fil: Tura Ceide, Olga. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: García Lucio, Jessica. Universidad de Barcelona; España
Fil: de la Cruz Thea, Benjamín Isaías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Meister, Gunter. Universitat Regensburg; Alemania
Fil: Loscalzo, Joseph. Brigham And Women's Hospital; Estados Unidos
Fil: Barberà, Joan A.. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Materia
COPD
MICRORNAS
PULMONARY ARTERY
SMOOTH MUSCLE CELL PHENOTYPIC SWITCH
VASCULAR REMODELING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96868
Acceder
id CONICETDig_6a8c28083d85a472eb5d3beeb1280f6e
oai_identifier_str oai:ri.conicet.gov.ar:11336/96868
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodelingMusri, Melina MaraColl Bonfill, NúriaMaron, Bradley A.Peinado, Victor IvoWang, Rui ShengAltirriba, JordiBlanco, IsabelOldham, William M.Tura Ceide, OlgaGarcía Lucio, Jessicade la Cruz Thea, Benjamín IsaíasMeister, GunterLoscalzo, JosephBarberà, Joan A.COPDMICRORNASPULMONARY ARTERYSMOOTH MUSCLE CELL PHENOTYPIC SWITCHVASCULAR REMODELINGhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR- 139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflowobstruction andPAintimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotypemeasured by both cell cycle analysis and wound-healing assay. Using luciferase assays, Western blot, and quantitative PCR, we confirmed that miR-197 targets the transcription factor E2F1. In PAs from patients with COPD, levels of E2F1 were increased as compared withNS. InPAs of patients with COPD, remodeling of the vesselwall is associated with downregulation of miR-197, which regulates SMC phenotype. The effect ofmiR-197 onPAsmight bemediated, at least in part, by the key proproliferative factor, E2F1.Fil: Musri, Melina Mara. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Coll Bonfill, Núria. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; EspañaFil: Maron, Bradley A.. Brigham And Women's Hospital; Estados UnidosFil: Peinado, Victor Ivo. Universidad de Barcelona; España. Biomedical Research Centre Network For Respiratory Diseases; EspañaFil: Wang, Rui Sheng. Brigham And Women's Hospital; Estados UnidosFil: Altirriba, Jordi. Universidad de Ginebra; SuizaFil: Blanco, Isabel. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; EspañaFil: Oldham, William M.. Brigham And Women's Hospital; Estados UnidosFil: Tura Ceide, Olga. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; EspañaFil: García Lucio, Jessica. Universidad de Barcelona; EspañaFil: de la Cruz Thea, Benjamín Isaías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Meister, Gunter. Universitat Regensburg; AlemaniaFil: Loscalzo, Joseph. Brigham And Women's Hospital; Estados UnidosFil: Barberà, Joan A.. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; EspañaAmerican Thoracic Society2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96868Musri, Melina Mara; Coll Bonfill, Núria; Maron, Bradley A.; Peinado, Victor Ivo; Wang, Rui Sheng; et al.; MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling; American Thoracic Society; American Journal Of Respiratory Cell And Molecular Biology; 59; 4; 10-2018; 490-4991044-1549CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1165/rcmb.2017-0040OCinfo:eu-repo/semantics/altIdentifier/url/https://www.atsjournals.org/doi/10.1165/rcmb.2017-0040OCinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:20Zoai:ri.conicet.gov.ar:11336/96868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:21.028CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
title MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
spellingShingle MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
Musri, Melina Mara
COPD
MICRORNAS
PULMONARY ARTERY
SMOOTH MUSCLE CELL PHENOTYPIC SWITCH
VASCULAR REMODELING
title_short MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
title_full MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
title_fullStr MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
title_full_unstemmed MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
title_sort MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
dc.creator.none.fl_str_mv Musri, Melina Mara
Coll Bonfill, Núria
Maron, Bradley A.
Peinado, Victor Ivo
Wang, Rui Sheng
Altirriba, Jordi
Blanco, Isabel
Oldham, William M.
Tura Ceide, Olga
García Lucio, Jessica
de la Cruz Thea, Benjamín Isaías
Meister, Gunter
Loscalzo, Joseph
Barberà, Joan A.
author Musri, Melina Mara
author_facet Musri, Melina Mara
Coll Bonfill, Núria
Maron, Bradley A.
Peinado, Victor Ivo
Wang, Rui Sheng
Altirriba, Jordi
Blanco, Isabel
Oldham, William M.
Tura Ceide, Olga
García Lucio, Jessica
de la Cruz Thea, Benjamín Isaías
Meister, Gunter
Loscalzo, Joseph
Barberà, Joan A.
author_role author
author2 Coll Bonfill, Núria
Maron, Bradley A.
Peinado, Victor Ivo
Wang, Rui Sheng
Altirriba, Jordi
Blanco, Isabel
Oldham, William M.
Tura Ceide, Olga
García Lucio, Jessica
de la Cruz Thea, Benjamín Isaías
Meister, Gunter
Loscalzo, Joseph
Barberà, Joan A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COPD
MICRORNAS
PULMONARY ARTERY
SMOOTH MUSCLE CELL PHENOTYPIC SWITCH
VASCULAR REMODELING
topic COPD
MICRORNAS
PULMONARY ARTERY
SMOOTH MUSCLE CELL PHENOTYPIC SWITCH
VASCULAR REMODELING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR- 139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflowobstruction andPAintimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotypemeasured by both cell cycle analysis and wound-healing assay. Using luciferase assays, Western blot, and quantitative PCR, we confirmed that miR-197 targets the transcription factor E2F1. In PAs from patients with COPD, levels of E2F1 were increased as compared withNS. InPAs of patients with COPD, remodeling of the vesselwall is associated with downregulation of miR-197, which regulates SMC phenotype. The effect ofmiR-197 onPAsmight bemediated, at least in part, by the key proproliferative factor, E2F1.
Fil: Musri, Melina Mara. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Coll Bonfill, Núria. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: Maron, Bradley A.. Brigham And Women's Hospital; Estados Unidos
Fil: Peinado, Victor Ivo. Universidad de Barcelona; España. Biomedical Research Centre Network For Respiratory Diseases; España
Fil: Wang, Rui Sheng. Brigham And Women's Hospital; Estados Unidos
Fil: Altirriba, Jordi. Universidad de Ginebra; Suiza
Fil: Blanco, Isabel. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: Oldham, William M.. Brigham And Women's Hospital; Estados Unidos
Fil: Tura Ceide, Olga. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
Fil: García Lucio, Jessica. Universidad de Barcelona; España
Fil: de la Cruz Thea, Benjamín Isaías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Meister, Gunter. Universitat Regensburg; Alemania
Fil: Loscalzo, Joseph. Brigham And Women's Hospital; Estados Unidos
Fil: Barberà, Joan A.. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
description Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR- 139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflowobstruction andPAintimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotypemeasured by both cell cycle analysis and wound-healing assay. Using luciferase assays, Western blot, and quantitative PCR, we confirmed that miR-197 targets the transcription factor E2F1. In PAs from patients with COPD, levels of E2F1 were increased as compared withNS. InPAs of patients with COPD, remodeling of the vesselwall is associated with downregulation of miR-197, which regulates SMC phenotype. The effect ofmiR-197 onPAsmight bemediated, at least in part, by the key proproliferative factor, E2F1.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96868
Musri, Melina Mara; Coll Bonfill, Núria; Maron, Bradley A.; Peinado, Victor Ivo; Wang, Rui Sheng; et al.; MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling; American Thoracic Society; American Journal Of Respiratory Cell And Molecular Biology; 59; 4; 10-2018; 490-499
1044-1549
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96868
identifier_str_mv Musri, Melina Mara; Coll Bonfill, Núria; Maron, Bradley A.; Peinado, Victor Ivo; Wang, Rui Sheng; et al.; MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling; American Thoracic Society; American Journal Of Respiratory Cell And Molecular Biology; 59; 4; 10-2018; 490-499
1044-1549
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1165/rcmb.2017-0040OC
info:eu-repo/semantics/altIdentifier/url/https://www.atsjournals.org/doi/10.1165/rcmb.2017-0040OC
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Thoracic Society
publisher.none.fl_str_mv American Thoracic Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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