17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins

Autores
Jerez S, Sierra L y Peral de Bruno M.; Jerez, Susana Josefina; Sierra, Liliana Beatríz; Peral de Bruno, María
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study investigated the role of CYP-enzymes in the modulation of vasoconstrictor responses to angiotensin II in rabbit aortae. In arteries with the endothelium-intact (E+) the CYP-inhibitor, 17-octadecynoic acid (17 ODYA), increased the efficacy to angiotensin II (17-ODYA-effect) as well as simultaneous incubation with miconazole (epoxygenase-inhibitor) and CAY 10434 (ω-hydroxylase-inhibitor). The removal of endothelium (E−) caused potentiation of the 17 ODYA-effect. Therefore, endothelium-dependent and -independent mechanisms would be involved. 17-ODYA and miconazole reduced Ach-relaxation. Indomethacin blocked the 17-ODYA-effect in E+ and E− arteries but blunted the response to angiotensin II only in E+ arteries. NS 398 (cyclooxygenase-2-inhibitor) blocked the 17-ODYA-effect and reduced angiotensin II affinity as well as SQ 29548 (thromboxane-prostanoid (TP) receptor-inhibitor). In E− arteries, CAY 10434 enhanced angiotensin II response as well as 17-ODYA. SC 560 (cyclooxygenase-1-inhibitor) and NS 398 partially blocked the 17-ODYA-effect. In conclusion, 17-ODYA induced endothelial dysfunction by inhibiting CYP-epoxygenase and thus improves vasoconstrictor cyclooxygenase-2 metabolites release acting through TP receptors. The endothelium-independent mechanism of 17-ODYA-effect may involve increase of vasoconstrictor cyclooxygenase-metabolites induced by prostaglandin-ω-hydroxylase-inhibition.
Fil: Jerez S, Sierra L y Peral de Bruno M.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sierra, Liliana Beatríz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral de Bruno, María. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Materia
Angiotensin Ii
Vascular Response
17-Octadecynoic
Cyclooxygenase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76947
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/76947
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandinsJerez S, Sierra L y Peral de Bruno M.Jerez, Susana JosefinaSierra, Liliana BeatrízPeral de Bruno, MaríaAngiotensin IiVascular Response17-OctadecynoicCyclooxygenasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study investigated the role of CYP-enzymes in the modulation of vasoconstrictor responses to angiotensin II in rabbit aortae. In arteries with the endothelium-intact (E+) the CYP-inhibitor, 17-octadecynoic acid (17 ODYA), increased the efficacy to angiotensin II (17-ODYA-effect) as well as simultaneous incubation with miconazole (epoxygenase-inhibitor) and CAY 10434 (ω-hydroxylase-inhibitor). The removal of endothelium (E−) caused potentiation of the 17 ODYA-effect. Therefore, endothelium-dependent and -independent mechanisms would be involved. 17-ODYA and miconazole reduced Ach-relaxation. Indomethacin blocked the 17-ODYA-effect in E+ and E− arteries but blunted the response to angiotensin II only in E+ arteries. NS 398 (cyclooxygenase-2-inhibitor) blocked the 17-ODYA-effect and reduced angiotensin II affinity as well as SQ 29548 (thromboxane-prostanoid (TP) receptor-inhibitor). In E− arteries, CAY 10434 enhanced angiotensin II response as well as 17-ODYA. SC 560 (cyclooxygenase-1-inhibitor) and NS 398 partially blocked the 17-ODYA-effect. In conclusion, 17-ODYA induced endothelial dysfunction by inhibiting CYP-epoxygenase and thus improves vasoconstrictor cyclooxygenase-2 metabolites release acting through TP receptors. The endothelium-independent mechanism of 17-ODYA-effect may involve increase of vasoconstrictor cyclooxygenase-metabolites induced by prostaglandin-ω-hydroxylase-inhibition.Fil: Jerez S, Sierra L y Peral de Bruno M.. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sierra, Liliana Beatríz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Peral de Bruno, María. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaElsevier Science Inc2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76947Jerez S, Sierra L y Peral de Bruno M.; Jerez, Susana Josefina; Sierra, Liliana Beatríz; Peral de Bruno, María; 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins; Elsevier Science Inc; Prostaglandins; 97; 1-2012; 36-421098-8823CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1098882311000700?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.prostaglandins.2011.07.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:51Zoai:ri.conicet.gov.ar:11336/76947instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:51.744CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
title 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
spellingShingle 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
Jerez S, Sierra L y Peral de Bruno M.
Angiotensin Ii
Vascular Response
17-Octadecynoic
Cyclooxygenase
title_short 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
title_full 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
title_fullStr 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
title_full_unstemmed 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
title_sort 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins
dc.creator.none.fl_str_mv Jerez S, Sierra L y Peral de Bruno M.
Jerez, Susana Josefina
Sierra, Liliana Beatríz
Peral de Bruno, María
author Jerez S, Sierra L y Peral de Bruno M.
author_facet Jerez S, Sierra L y Peral de Bruno M.
Jerez, Susana Josefina
Sierra, Liliana Beatríz
Peral de Bruno, María
author_role author
author2 Jerez, Susana Josefina
Sierra, Liliana Beatríz
Peral de Bruno, María
author2_role author
author
author
dc.subject.none.fl_str_mv Angiotensin Ii
Vascular Response
17-Octadecynoic
Cyclooxygenase
topic Angiotensin Ii
Vascular Response
17-Octadecynoic
Cyclooxygenase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study investigated the role of CYP-enzymes in the modulation of vasoconstrictor responses to angiotensin II in rabbit aortae. In arteries with the endothelium-intact (E+) the CYP-inhibitor, 17-octadecynoic acid (17 ODYA), increased the efficacy to angiotensin II (17-ODYA-effect) as well as simultaneous incubation with miconazole (epoxygenase-inhibitor) and CAY 10434 (ω-hydroxylase-inhibitor). The removal of endothelium (E−) caused potentiation of the 17 ODYA-effect. Therefore, endothelium-dependent and -independent mechanisms would be involved. 17-ODYA and miconazole reduced Ach-relaxation. Indomethacin blocked the 17-ODYA-effect in E+ and E− arteries but blunted the response to angiotensin II only in E+ arteries. NS 398 (cyclooxygenase-2-inhibitor) blocked the 17-ODYA-effect and reduced angiotensin II affinity as well as SQ 29548 (thromboxane-prostanoid (TP) receptor-inhibitor). In E− arteries, CAY 10434 enhanced angiotensin II response as well as 17-ODYA. SC 560 (cyclooxygenase-1-inhibitor) and NS 398 partially blocked the 17-ODYA-effect. In conclusion, 17-ODYA induced endothelial dysfunction by inhibiting CYP-epoxygenase and thus improves vasoconstrictor cyclooxygenase-2 metabolites release acting through TP receptors. The endothelium-independent mechanism of 17-ODYA-effect may involve increase of vasoconstrictor cyclooxygenase-metabolites induced by prostaglandin-ω-hydroxylase-inhibition.
Fil: Jerez S, Sierra L y Peral de Bruno M.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sierra, Liliana Beatríz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral de Bruno, María. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
description The present study investigated the role of CYP-enzymes in the modulation of vasoconstrictor responses to angiotensin II in rabbit aortae. In arteries with the endothelium-intact (E+) the CYP-inhibitor, 17-octadecynoic acid (17 ODYA), increased the efficacy to angiotensin II (17-ODYA-effect) as well as simultaneous incubation with miconazole (epoxygenase-inhibitor) and CAY 10434 (ω-hydroxylase-inhibitor). The removal of endothelium (E−) caused potentiation of the 17 ODYA-effect. Therefore, endothelium-dependent and -independent mechanisms would be involved. 17-ODYA and miconazole reduced Ach-relaxation. Indomethacin blocked the 17-ODYA-effect in E+ and E− arteries but blunted the response to angiotensin II only in E+ arteries. NS 398 (cyclooxygenase-2-inhibitor) blocked the 17-ODYA-effect and reduced angiotensin II affinity as well as SQ 29548 (thromboxane-prostanoid (TP) receptor-inhibitor). In E− arteries, CAY 10434 enhanced angiotensin II response as well as 17-ODYA. SC 560 (cyclooxygenase-1-inhibitor) and NS 398 partially blocked the 17-ODYA-effect. In conclusion, 17-ODYA induced endothelial dysfunction by inhibiting CYP-epoxygenase and thus improves vasoconstrictor cyclooxygenase-2 metabolites release acting through TP receptors. The endothelium-independent mechanism of 17-ODYA-effect may involve increase of vasoconstrictor cyclooxygenase-metabolites induced by prostaglandin-ω-hydroxylase-inhibition.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76947
Jerez S, Sierra L y Peral de Bruno M.; Jerez, Susana Josefina; Sierra, Liliana Beatríz; Peral de Bruno, María; 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins; Elsevier Science Inc; Prostaglandins; 97; 1-2012; 36-42
1098-8823
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76947
identifier_str_mv Jerez S, Sierra L y Peral de Bruno M.; Jerez, Susana Josefina; Sierra, Liliana Beatríz; Peral de Bruno, María; 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins; Elsevier Science Inc; Prostaglandins; 97; 1-2012; 36-42
1098-8823
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1098882311000700?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.prostaglandins.2011.07.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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