Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats

Autores
Meneghini, María Agustina; Flores Quiroga, Jeremias Pablo; Analia Elizabeth, Cortez; Faletti, Alicia Graciela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Obesity has increased in recent years and is the most importantnoncommunicable chronic disease. Maternal overnutrition may inducemultiple pathologies in both women and their offspring. Ourprevious studies showed that male offspring from high-fat-fed ratsexhibited higher body and testis weight and altered puberty. Also,we found a lower number of germ cells, percentage of motile spermand capacitation. Thus, the aim of the present study was to evaluatethe effects of maternal overnutrition, induced by high-fat diet, on thequality and function of sperm in the offspring.To this end, maternalovernutrition were induced by a high-fat palatable (cafeteria) diet,which was supplied continuously until weaning of their offspring, includingpregnancy and lactation. Male offspring from rats fed standard(OSD) or cafeteria diet (OCD) were fed with a standard diet, inspectedperiodically, and euthanized at 60 days of age. In the germcells we examined the presence of the reactive oxygen species byflow cytometry using a fluorescent probe (2,7-dichlorofluorescein diacetate),DNA fragmentation by TUNEL kit, mitochondrial functionusing the probe 3.3-diaminobenzidine, the membrane functionalstatus by hypoosmotic swelling test, and the presence of abnormalchromosomes by cytogenetic assay (Evan test). Compared withOSD rats, OCD group showed a lower percentage of the hypoosmotic-reacted sperm (15±1 vs 23±2, p<0.01) and an increase in theabnormal metaphases (6±1 vs 2.1±0.7, p<0.001). No differenceswere found in the TUNEL positive cells, but OCD exhibited higherfluorescein intensity expressed as relative units (577±74, p<0.01)compared with OSD (233±27). Finally, 50% of OCD rats displayeda lower mitochondrial function, expressed as relative units (94.8±4vs 97.7±0.4 from OSD, p<0.01).These results indicate that diet-inducedmaternal overnutrition may contribute to disorders in the fetalprogramming, particularly in the germ cell quality.
Fil: Meneghini, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Flores Quiroga, Jeremias Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Analia Elizabeth, Cortez. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Faletti, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
Obesity
Sperm quality
Offspring
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197725
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197725
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disruption in the sperm quality of the offspring caused by maternal overnutrition in ratsMeneghini, María AgustinaFlores Quiroga, Jeremias PabloAnalia Elizabeth, CortezFaletti, Alicia GracielaObesitySperm qualityOffspringhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Obesity has increased in recent years and is the most importantnoncommunicable chronic disease. Maternal overnutrition may inducemultiple pathologies in both women and their offspring. Ourprevious studies showed that male offspring from high-fat-fed ratsexhibited higher body and testis weight and altered puberty. Also,we found a lower number of germ cells, percentage of motile spermand capacitation. Thus, the aim of the present study was to evaluatethe effects of maternal overnutrition, induced by high-fat diet, on thequality and function of sperm in the offspring.To this end, maternalovernutrition were induced by a high-fat palatable (cafeteria) diet,which was supplied continuously until weaning of their offspring, includingpregnancy and lactation. Male offspring from rats fed standard(OSD) or cafeteria diet (OCD) were fed with a standard diet, inspectedperiodically, and euthanized at 60 days of age. In the germcells we examined the presence of the reactive oxygen species byflow cytometry using a fluorescent probe (2,7-dichlorofluorescein diacetate),DNA fragmentation by TUNEL kit, mitochondrial functionusing the probe 3.3-diaminobenzidine, the membrane functionalstatus by hypoosmotic swelling test, and the presence of abnormalchromosomes by cytogenetic assay (Evan test). Compared withOSD rats, OCD group showed a lower percentage of the hypoosmotic-reacted sperm (15±1 vs 23±2, p<0.01) and an increase in theabnormal metaphases (6±1 vs 2.1±0.7, p<0.001). No differenceswere found in the TUNEL positive cells, but OCD exhibited higherfluorescein intensity expressed as relative units (577±74, p<0.01)compared with OSD (233±27). Finally, 50% of OCD rats displayeda lower mitochondrial function, expressed as relative units (94.8±4vs 97.7±0.4 from OSD, p<0.01).These results indicate that diet-inducedmaternal overnutrition may contribute to disorders in the fetalprogramming, particularly in the germ cell quality.Fil: Meneghini, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Flores Quiroga, Jeremias Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Analia Elizabeth, Cortez. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Faletti, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de Investigación clínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197725Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 1-10025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:50Zoai:ri.conicet.gov.ar:11336/197725instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:50.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
title Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
spellingShingle Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
Meneghini, María Agustina
Obesity
Sperm quality
Offspring
title_short Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
title_full Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
title_fullStr Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
title_full_unstemmed Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
title_sort Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats
dc.creator.none.fl_str_mv Meneghini, María Agustina
Flores Quiroga, Jeremias Pablo
Analia Elizabeth, Cortez
Faletti, Alicia Graciela
author Meneghini, María Agustina
author_facet Meneghini, María Agustina
Flores Quiroga, Jeremias Pablo
Analia Elizabeth, Cortez
Faletti, Alicia Graciela
author_role author
author2 Flores Quiroga, Jeremias Pablo
Analia Elizabeth, Cortez
Faletti, Alicia Graciela
author2_role author
author
author
dc.subject.none.fl_str_mv Obesity
Sperm quality
Offspring
topic Obesity
Sperm quality
Offspring
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Obesity has increased in recent years and is the most importantnoncommunicable chronic disease. Maternal overnutrition may inducemultiple pathologies in both women and their offspring. Ourprevious studies showed that male offspring from high-fat-fed ratsexhibited higher body and testis weight and altered puberty. Also,we found a lower number of germ cells, percentage of motile spermand capacitation. Thus, the aim of the present study was to evaluatethe effects of maternal overnutrition, induced by high-fat diet, on thequality and function of sperm in the offspring.To this end, maternalovernutrition were induced by a high-fat palatable (cafeteria) diet,which was supplied continuously until weaning of their offspring, includingpregnancy and lactation. Male offspring from rats fed standard(OSD) or cafeteria diet (OCD) were fed with a standard diet, inspectedperiodically, and euthanized at 60 days of age. In the germcells we examined the presence of the reactive oxygen species byflow cytometry using a fluorescent probe (2,7-dichlorofluorescein diacetate),DNA fragmentation by TUNEL kit, mitochondrial functionusing the probe 3.3-diaminobenzidine, the membrane functionalstatus by hypoosmotic swelling test, and the presence of abnormalchromosomes by cytogenetic assay (Evan test). Compared withOSD rats, OCD group showed a lower percentage of the hypoosmotic-reacted sperm (15±1 vs 23±2, p<0.01) and an increase in theabnormal metaphases (6±1 vs 2.1±0.7, p<0.001). No differenceswere found in the TUNEL positive cells, but OCD exhibited higherfluorescein intensity expressed as relative units (577±74, p<0.01)compared with OSD (233±27). Finally, 50% of OCD rats displayeda lower mitochondrial function, expressed as relative units (94.8±4vs 97.7±0.4 from OSD, p<0.01).These results indicate that diet-inducedmaternal overnutrition may contribute to disorders in the fetalprogramming, particularly in the germ cell quality.
Fil: Meneghini, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Flores Quiroga, Jeremias Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Analia Elizabeth, Cortez. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Faletti, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Obesity has increased in recent years and is the most importantnoncommunicable chronic disease. Maternal overnutrition may inducemultiple pathologies in both women and their offspring. Ourprevious studies showed that male offspring from high-fat-fed ratsexhibited higher body and testis weight and altered puberty. Also,we found a lower number of germ cells, percentage of motile spermand capacitation. Thus, the aim of the present study was to evaluatethe effects of maternal overnutrition, induced by high-fat diet, on thequality and function of sperm in the offspring.To this end, maternalovernutrition were induced by a high-fat palatable (cafeteria) diet,which was supplied continuously until weaning of their offspring, includingpregnancy and lactation. Male offspring from rats fed standard(OSD) or cafeteria diet (OCD) were fed with a standard diet, inspectedperiodically, and euthanized at 60 days of age. In the germcells we examined the presence of the reactive oxygen species byflow cytometry using a fluorescent probe (2,7-dichlorofluorescein diacetate),DNA fragmentation by TUNEL kit, mitochondrial functionusing the probe 3.3-diaminobenzidine, the membrane functionalstatus by hypoosmotic swelling test, and the presence of abnormalchromosomes by cytogenetic assay (Evan test). Compared withOSD rats, OCD group showed a lower percentage of the hypoosmotic-reacted sperm (15±1 vs 23±2, p<0.01) and an increase in theabnormal metaphases (6±1 vs 2.1±0.7, p<0.001). No differenceswere found in the TUNEL positive cells, but OCD exhibited higherfluorescein intensity expressed as relative units (577±74, p<0.01)compared with OSD (233±27). Finally, 50% of OCD rats displayeda lower mitochondrial function, expressed as relative units (94.8±4vs 97.7±0.4 from OSD, p<0.01).These results indicate that diet-inducedmaternal overnutrition may contribute to disorders in the fetalprogramming, particularly in the germ cell quality.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197725
Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 1-1
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197725
identifier_str_mv Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 1-1
0025-7680
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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