Diverging biological roles among human monocyte subsets in the context of tuberculosis infection

Autores
Balboa, Luciana; Barrios Payan, Jorge; González Domínguez, Erika; Lastrucci, Claire; Lugo Villarino, Geanncarlo; Mata Espinoza, Dulce; Schierloh, Luis Pablo; Kviatcovsky, Denise; Neyrolles, Olivier; Maridonneau Parini, Isabelle; Sánchez Torres, Carmen; Sasiain, María del Carmen; Hernández Pando, Rogelio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16pos Mo were expanded in TB (tuberculosis) patients, correlated with disease severity and were refractory to dendritic cell differentiation. In the present study, we investigated whether human Mo subsets (CD16neg and CD16pos) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis. We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16neg Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to M. tuberculosis intracellular growth and produced higher reactive oxygen species than their CD16pos counterpart. To assess further the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige, where SCID is severe combined immunodeficient) to transfer each Mo subset, track their migratory fate during M. tuberculosis infection, and determine their impact on the host immune response. In M. tuberculosis-infected mice, the adoptively transferred CD16neg Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leucocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison with CD16pos Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of M. tuberculosis infection, a scenario in which CD16neg Mo may contribute to the anti-mycobacterial immune response, whereas CD16pos Mo might promote microbial resilience, shedding light on a key aspect of the physiopathology of TB disease.
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Barrios Payan, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: González Domínguez, Erika. Instituto Politécnico Nacional; México
Fil: Lastrucci, Claire. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Lugo Villarino, Geanncarlo. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Mata Espinoza, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Kviatcovsky, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Neyrolles, Olivier. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Maridonneau Parini, Isabelle. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Sánchez Torres, Carmen. Instituto Politécnico Nacional; México
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Materia
Bacterial Infection
Cell Movement
Monocytes
Mycobacterium Infection
Pulmonary Infection
Tuberculosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39825
Acceder
id CONICETDig_69ef811580d5b056e511432a8e01b52d
oai_identifier_str oai:ri.conicet.gov.ar:11336/39825
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diverging biological roles among human monocyte subsets in the context of tuberculosis infectionBalboa, LucianaBarrios Payan, JorgeGonzález Domínguez, ErikaLastrucci, ClaireLugo Villarino, GeanncarloMata Espinoza, DulceSchierloh, Luis PabloKviatcovsky, DeniseNeyrolles, OlivierMaridonneau Parini, IsabelleSánchez Torres, CarmenSasiain, María del CarmenHernández Pando, RogelioBacterial InfectionCell MovementMonocytesMycobacterium InfectionPulmonary InfectionTuberculosishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16pos Mo were expanded in TB (tuberculosis) patients, correlated with disease severity and were refractory to dendritic cell differentiation. In the present study, we investigated whether human Mo subsets (CD16neg and CD16pos) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis. We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16neg Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to M. tuberculosis intracellular growth and produced higher reactive oxygen species than their CD16pos counterpart. To assess further the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige, where SCID is severe combined immunodeficient) to transfer each Mo subset, track their migratory fate during M. tuberculosis infection, and determine their impact on the host immune response. In M. tuberculosis-infected mice, the adoptively transferred CD16neg Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leucocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison with CD16pos Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of M. tuberculosis infection, a scenario in which CD16neg Mo may contribute to the anti-mycobacterial immune response, whereas CD16pos Mo might promote microbial resilience, shedding light on a key aspect of the physiopathology of TB disease.Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Barrios Payan, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: González Domínguez, Erika. Instituto Politécnico Nacional; MéxicoFil: Lastrucci, Claire. Institut de Pharmacologie et de Biologie Structurale; FranciaFil: Lugo Villarino, Geanncarlo. Institut de Pharmacologie et de Biologie Structurale; FranciaFil: Mata Espinoza, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Kviatcovsky, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Neyrolles, Olivier. Institut de Pharmacologie et de Biologie Structurale; FranciaFil: Maridonneau Parini, Isabelle. Institut de Pharmacologie et de Biologie Structurale; FranciaFil: Sánchez Torres, Carmen. Instituto Politécnico Nacional; MéxicoFil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoPortland Press2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39825Balboa, Luciana; Barrios Payan, Jorge; González Domínguez, Erika; Lastrucci, Claire; Lugo Villarino, Geanncarlo; et al.; Diverging biological roles among human monocyte subsets in the context of tuberculosis infection; Portland Press; Clinical Science; 129; 4; 4-2015; 319-3300143-5221CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1042/CS20150021info:eu-repo/semantics/altIdentifier/url/http://www.clinsci.org/content/129/4/319info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:36Zoai:ri.conicet.gov.ar:11336/39825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:36.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
title Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
spellingShingle Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
Balboa, Luciana
Bacterial Infection
Cell Movement
Monocytes
Mycobacterium Infection
Pulmonary Infection
Tuberculosis
title_short Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
title_full Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
title_fullStr Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
title_full_unstemmed Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
title_sort Diverging biological roles among human monocyte subsets in the context of tuberculosis infection
dc.creator.none.fl_str_mv Balboa, Luciana
Barrios Payan, Jorge
González Domínguez, Erika
Lastrucci, Claire
Lugo Villarino, Geanncarlo
Mata Espinoza, Dulce
Schierloh, Luis Pablo
Kviatcovsky, Denise
Neyrolles, Olivier
Maridonneau Parini, Isabelle
Sánchez Torres, Carmen
Sasiain, María del Carmen
Hernández Pando, Rogelio
author Balboa, Luciana
author_facet Balboa, Luciana
Barrios Payan, Jorge
González Domínguez, Erika
Lastrucci, Claire
Lugo Villarino, Geanncarlo
Mata Espinoza, Dulce
Schierloh, Luis Pablo
Kviatcovsky, Denise
Neyrolles, Olivier
Maridonneau Parini, Isabelle
Sánchez Torres, Carmen
Sasiain, María del Carmen
Hernández Pando, Rogelio
author_role author
author2 Barrios Payan, Jorge
González Domínguez, Erika
Lastrucci, Claire
Lugo Villarino, Geanncarlo
Mata Espinoza, Dulce
Schierloh, Luis Pablo
Kviatcovsky, Denise
Neyrolles, Olivier
Maridonneau Parini, Isabelle
Sánchez Torres, Carmen
Sasiain, María del Carmen
Hernández Pando, Rogelio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial Infection
Cell Movement
Monocytes
Mycobacterium Infection
Pulmonary Infection
Tuberculosis
topic Bacterial Infection
Cell Movement
Monocytes
Mycobacterium Infection
Pulmonary Infection
Tuberculosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16pos Mo were expanded in TB (tuberculosis) patients, correlated with disease severity and were refractory to dendritic cell differentiation. In the present study, we investigated whether human Mo subsets (CD16neg and CD16pos) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis. We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16neg Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to M. tuberculosis intracellular growth and produced higher reactive oxygen species than their CD16pos counterpart. To assess further the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige, where SCID is severe combined immunodeficient) to transfer each Mo subset, track their migratory fate during M. tuberculosis infection, and determine their impact on the host immune response. In M. tuberculosis-infected mice, the adoptively transferred CD16neg Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leucocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison with CD16pos Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of M. tuberculosis infection, a scenario in which CD16neg Mo may contribute to the anti-mycobacterial immune response, whereas CD16pos Mo might promote microbial resilience, shedding light on a key aspect of the physiopathology of TB disease.
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Barrios Payan, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: González Domínguez, Erika. Instituto Politécnico Nacional; México
Fil: Lastrucci, Claire. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Lugo Villarino, Geanncarlo. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Mata Espinoza, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Kviatcovsky, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Neyrolles, Olivier. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Maridonneau Parini, Isabelle. Institut de Pharmacologie et de Biologie Structurale; Francia
Fil: Sánchez Torres, Carmen. Instituto Politécnico Nacional; México
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
description Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16pos Mo were expanded in TB (tuberculosis) patients, correlated with disease severity and were refractory to dendritic cell differentiation. In the present study, we investigated whether human Mo subsets (CD16neg and CD16pos) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis. We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16neg Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to M. tuberculosis intracellular growth and produced higher reactive oxygen species than their CD16pos counterpart. To assess further the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige, where SCID is severe combined immunodeficient) to transfer each Mo subset, track their migratory fate during M. tuberculosis infection, and determine their impact on the host immune response. In M. tuberculosis-infected mice, the adoptively transferred CD16neg Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leucocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison with CD16pos Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of M. tuberculosis infection, a scenario in which CD16neg Mo may contribute to the anti-mycobacterial immune response, whereas CD16pos Mo might promote microbial resilience, shedding light on a key aspect of the physiopathology of TB disease.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39825
Balboa, Luciana; Barrios Payan, Jorge; González Domínguez, Erika; Lastrucci, Claire; Lugo Villarino, Geanncarlo; et al.; Diverging biological roles among human monocyte subsets in the context of tuberculosis infection; Portland Press; Clinical Science; 129; 4; 4-2015; 319-330
0143-5221
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39825
identifier_str_mv Balboa, Luciana; Barrios Payan, Jorge; González Domínguez, Erika; Lastrucci, Claire; Lugo Villarino, Geanncarlo; et al.; Diverging biological roles among human monocyte subsets in the context of tuberculosis infection; Portland Press; Clinical Science; 129; 4; 4-2015; 319-330
0143-5221
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1042/CS20150021
info:eu-repo/semantics/altIdentifier/url/http://www.clinsci.org/content/129/4/319
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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