Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse

Autores
Pérez Tito, Leticia Gabriela; Bevilacqua, Estela; Cebral, Elisa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p < 0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p < 0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p < 0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p < 0.05) versus controls, whereas embryo type 2 percentage increased (p < 0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p < 0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4–5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption.
Fil: Pérez Tito, Leticia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Bevilacqua, Estela. Universidade de Sao Paulo; Brasil
Fil: Cebral, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Alcohol
Blastocyst
Differentiation
Growth
Mouse
Trophoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31940
Acceder
id CONICETDig_69e131aaa348867e8ff84416c60e9eb2
oai_identifier_str oai:ri.conicet.gov.ar:11336/31940
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mousePérez Tito, Leticia GabrielaBevilacqua, EstelaCebral, ElisaAlcoholBlastocystDifferentiationGrowthMouseTrophoblasthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p < 0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p < 0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p < 0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p < 0.05) versus controls, whereas embryo type 2 percentage increased (p < 0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p < 0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4–5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption.Fil: Pérez Tito, Leticia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Bevilacqua, Estela. Universidade de Sao Paulo; BrasilFil: Cebral, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaTaylor2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31940Cebral, Elisa; Bevilacqua, Estela; Pérez Tito, Leticia Gabriela; Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse; Taylor; Drug And Chemical Toxicology; 37; 2; 10-2014; 184-1970148-0545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/01480545.2013.834358info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/01480545.2013.834358info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:45:39Zoai:ri.conicet.gov.ar:11336/31940instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:45:40.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
title Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
spellingShingle Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
Pérez Tito, Leticia Gabriela
Alcohol
Blastocyst
Differentiation
Growth
Mouse
Trophoblast
title_short Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
title_full Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
title_fullStr Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
title_full_unstemmed Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
title_sort Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
dc.creator.none.fl_str_mv Pérez Tito, Leticia Gabriela
Bevilacqua, Estela
Cebral, Elisa
author Pérez Tito, Leticia Gabriela
author_facet Pérez Tito, Leticia Gabriela
Bevilacqua, Estela
Cebral, Elisa
author_role author
author2 Bevilacqua, Estela
Cebral, Elisa
author2_role author
author
dc.subject.none.fl_str_mv Alcohol
Blastocyst
Differentiation
Growth
Mouse
Trophoblast
topic Alcohol
Blastocyst
Differentiation
Growth
Mouse
Trophoblast
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p < 0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p < 0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p < 0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p < 0.05) versus controls, whereas embryo type 2 percentage increased (p < 0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p < 0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4–5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption.
Fil: Pérez Tito, Leticia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Bevilacqua, Estela. Universidade de Sao Paulo; Brasil
Fil: Cebral, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p < 0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p < 0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p < 0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p < 0.05) versus controls, whereas embryo type 2 percentage increased (p < 0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p < 0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4–5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31940
Cebral, Elisa; Bevilacqua, Estela; Pérez Tito, Leticia Gabriela; Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse; Taylor; Drug And Chemical Toxicology; 37; 2; 10-2014; 184-197
0148-0545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31940
identifier_str_mv Cebral, Elisa; Bevilacqua, Estela; Pérez Tito, Leticia Gabriela; Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse; Taylor; Drug And Chemical Toxicology; 37; 2; 10-2014; 184-197
0148-0545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/01480545.2013.834358
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/01480545.2013.834358
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor
publisher.none.fl_str_mv Taylor
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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