The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action

Autores
Goldoni Lazarini, Josy; Prado Massarioli, Adna; Cintra Soares, Jackeline; Dias Nani, Bruno; Charó, Nancy Lorena; Souza Oliveira, Douglas; Camargo, Lauren; Alvarez Flores, Miryam Paola; de Fatima Correia Batista, Isabel; Chudzinski Tavassi, Ana Marisa; de Alencar, Severino Matias; Franchin, Marcelo; Rosalen, Pedro Luiz
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.
Fil: Goldoni Lazarini, Josy. Universidade Estadual de Campinas; Brasil
Fil: Prado Massarioli, Adna. Universidade de Sao Paulo; Brasil
Fil: Cintra Soares, Jackeline. Universidade de Sao Paulo; Brasil
Fil: Dias Nani, Bruno. Universidade Estadual de Campinas; Brasil
Fil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Souza Oliveira, Douglas. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Camargo, Lauren. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Alvarez Flores, Miryam Paola. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: de Fatima Correia Batista, Isabel. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Chudzinski Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: de Alencar, Severino Matias. Universidade de Sao Paulo; Brasil
Fil: Franchin, Marcelo. Universidade Estadual de Campinas; Brasil
Fil: Rosalen, Pedro Luiz. Universidade Estadual de Campinas; Brasil
Materia
ANTI-INFLAMMATORY
EUGENIA SELLOI
NATIVE FRUIT
ROS AND RNS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210647
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210647
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of actionGoldoni Lazarini, JosyPrado Massarioli, AdnaCintra Soares, JackelineDias Nani, BrunoCharó, Nancy LorenaSouza Oliveira, DouglasCamargo, LaurenAlvarez Flores, Miryam Paolade Fatima Correia Batista, IsabelChudzinski Tavassi, Ana Marisade Alencar, Severino MatiasFranchin, MarceloRosalen, Pedro LuizANTI-INFLAMMATORYEUGENIA SELLOINATIVE FRUITROS AND RNShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.Fil: Goldoni Lazarini, Josy. Universidade Estadual de Campinas; BrasilFil: Prado Massarioli, Adna. Universidade de Sao Paulo; BrasilFil: Cintra Soares, Jackeline. Universidade de Sao Paulo; BrasilFil: Dias Nani, Bruno. Universidade Estadual de Campinas; BrasilFil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Souza Oliveira, Douglas. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Camargo, Lauren. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Alvarez Flores, Miryam Paola. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: de Fatima Correia Batista, Isabel. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Chudzinski Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: de Alencar, Severino Matias. Universidade de Sao Paulo; BrasilFil: Franchin, Marcelo. Universidade Estadual de Campinas; BrasilFil: Rosalen, Pedro Luiz. Universidade Estadual de Campinas; BrasilElsevier2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210647Goldoni Lazarini, Josy; Prado Massarioli, Adna; Cintra Soares, Jackeline; Dias Nani, Bruno; Charó, Nancy Lorena; et al.; The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action; Elsevier; Food Chemistry: Molecular Sciences; 4; 7-2022; 1-102666-5662CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fochms.2022.100093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:58Zoai:ri.conicet.gov.ar:11336/210647instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:58.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
title The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
spellingShingle The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
Goldoni Lazarini, Josy
ANTI-INFLAMMATORY
EUGENIA SELLOI
NATIVE FRUIT
ROS AND RNS
title_short The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
title_full The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
title_fullStr The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
title_full_unstemmed The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
title_sort The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action
dc.creator.none.fl_str_mv Goldoni Lazarini, Josy
Prado Massarioli, Adna
Cintra Soares, Jackeline
Dias Nani, Bruno
Charó, Nancy Lorena
Souza Oliveira, Douglas
Camargo, Lauren
Alvarez Flores, Miryam Paola
de Fatima Correia Batista, Isabel
Chudzinski Tavassi, Ana Marisa
de Alencar, Severino Matias
Franchin, Marcelo
Rosalen, Pedro Luiz
author Goldoni Lazarini, Josy
author_facet Goldoni Lazarini, Josy
Prado Massarioli, Adna
Cintra Soares, Jackeline
Dias Nani, Bruno
Charó, Nancy Lorena
Souza Oliveira, Douglas
Camargo, Lauren
Alvarez Flores, Miryam Paola
de Fatima Correia Batista, Isabel
Chudzinski Tavassi, Ana Marisa
de Alencar, Severino Matias
Franchin, Marcelo
Rosalen, Pedro Luiz
author_role author
author2 Prado Massarioli, Adna
Cintra Soares, Jackeline
Dias Nani, Bruno
Charó, Nancy Lorena
Souza Oliveira, Douglas
Camargo, Lauren
Alvarez Flores, Miryam Paola
de Fatima Correia Batista, Isabel
Chudzinski Tavassi, Ana Marisa
de Alencar, Severino Matias
Franchin, Marcelo
Rosalen, Pedro Luiz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTI-INFLAMMATORY
EUGENIA SELLOI
NATIVE FRUIT
ROS AND RNS
topic ANTI-INFLAMMATORY
EUGENIA SELLOI
NATIVE FRUIT
ROS AND RNS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.
Fil: Goldoni Lazarini, Josy. Universidade Estadual de Campinas; Brasil
Fil: Prado Massarioli, Adna. Universidade de Sao Paulo; Brasil
Fil: Cintra Soares, Jackeline. Universidade de Sao Paulo; Brasil
Fil: Dias Nani, Bruno. Universidade Estadual de Campinas; Brasil
Fil: Charó, Nancy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Souza Oliveira, Douglas. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Camargo, Lauren. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Alvarez Flores, Miryam Paola. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: de Fatima Correia Batista, Isabel. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Chudzinski Tavassi, Ana Marisa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: de Alencar, Severino Matias. Universidade de Sao Paulo; Brasil
Fil: Franchin, Marcelo. Universidade Estadual de Campinas; Brasil
Fil: Rosalen, Pedro Luiz. Universidade Estadual de Campinas; Brasil
description We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.
publishDate 2022
dc.date.none.fl_str_mv 2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210647
Goldoni Lazarini, Josy; Prado Massarioli, Adna; Cintra Soares, Jackeline; Dias Nani, Bruno; Charó, Nancy Lorena; et al.; The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action; Elsevier; Food Chemistry: Molecular Sciences; 4; 7-2022; 1-10
2666-5662
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210647
identifier_str_mv Goldoni Lazarini, Josy; Prado Massarioli, Adna; Cintra Soares, Jackeline; Dias Nani, Bruno; Charó, Nancy Lorena; et al.; The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): Toxicity and elucidation of their anti-inflammatory mechanism(s) of action; Elsevier; Food Chemistry: Molecular Sciences; 4; 7-2022; 1-10
2666-5662
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fochms.2022.100093
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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