Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa

Autores
Bobadilla, Fernando Javier; Novosak, Marina Gisel; Winnik, Daniana L.; Kachuk, Analia V.; Laczeski, Margarita Ester; Quiroga, Marina I.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas aeruginosa is one of the main causes of nosocomial infections worldwide, with great potential for multi-resistance development. The World Health Organization (WHO) considers the antimicrobial resistance as an emerging public health problem that makes new therapies an extremely urgent issue. The aim of this work was to study the antibacterial capacity of the ethanolic extract of Eugenia uniflora L. leaves on Pseudomonas aeruginosa, its possible synergistic action with commercial antibiotics and to carry out general toxicity tests using the crustacean Artemia salina. The extract had a Minimum Inhibitory Dose (MID) of 0.5 mg.disc-1. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) for P. aeruginosa PAO1 were 1.33 mg.ml-1 and 13.33 mg.ml-1. For the clinical isolations were 0.83 mg.ml-1 and 8.33 mg.ml-1. The MBC/MIC ratio (MICI) qualified the action of the extract as bacteriostatic. The Time-killing curve showed that the total loss of viability of the bacteria occurs at 24 h to 16 mg.ml-1 and at 8 h to 32 mg.ml-1. The combination of meropenem with the extract results in a decrease in its inhibitory action on P. aeruginosa PAO1. Meanwhile, the combination with piperacycline or ceftazidime produces a synergistic action. For ciprofloxacin, amicacin and colistin the combination is indifferent. The LC50 obtained using A. salina was 0.61 mg.ml-1; indicating that the extract has a moderate toxicity. These results suggest that this species would be a potential source of antimicrobial agents to be applied in the treatment of infections caused by P. aeruginosa.
Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Winnik, Daniana L.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Kachuk, Analia V.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Quiroga, Marina I.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Materia
ANTIBACTERIAL ACTIVITY
ETHANOLIC EXTRACT
EUGENIA UNIFLORA L
PSEUDOMONAS AERUGINOSA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/83936
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas AeruginosaBobadilla, Fernando JavierNovosak, Marina GiselWinnik, Daniana L.Kachuk, Analia V.Laczeski, Margarita EsterQuiroga, Marina I.ANTIBACTERIAL ACTIVITYETHANOLIC EXTRACTEUGENIA UNIFLORA LPSEUDOMONAS AERUGINOSAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudomonas aeruginosa is one of the main causes of nosocomial infections worldwide, with great potential for multi-resistance development. The World Health Organization (WHO) considers the antimicrobial resistance as an emerging public health problem that makes new therapies an extremely urgent issue. The aim of this work was to study the antibacterial capacity of the ethanolic extract of Eugenia uniflora L. leaves on Pseudomonas aeruginosa, its possible synergistic action with commercial antibiotics and to carry out general toxicity tests using the crustacean Artemia salina. The extract had a Minimum Inhibitory Dose (MID) of 0.5 mg.disc-1. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) for P. aeruginosa PAO1 were 1.33 mg.ml-1 and 13.33 mg.ml-1. For the clinical isolations were 0.83 mg.ml-1 and 8.33 mg.ml-1. The MBC/MIC ratio (MICI) qualified the action of the extract as bacteriostatic. The Time-killing curve showed that the total loss of viability of the bacteria occurs at 24 h to 16 mg.ml-1 and at 8 h to 32 mg.ml-1. The combination of meropenem with the extract results in a decrease in its inhibitory action on P. aeruginosa PAO1. Meanwhile, the combination with piperacycline or ceftazidime produces a synergistic action. For ciprofloxacin, amicacin and colistin the combination is indifferent. The LC50 obtained using A. salina was 0.61 mg.ml-1; indicating that the extract has a moderate toxicity. These results suggest that this species would be a potential source of antimicrobial agents to be applied in the treatment of infections caused by P. aeruginosa.Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaFil: Winnik, Daniana L.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaFil: Kachuk, Analia V.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaFil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaFil: Quiroga, Marina I.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; ArgentinaSlovak University of Agriculture2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83936Bobadilla, Fernando Javier; Novosak, Marina Gisel; Winnik, Daniana L.; Kachuk, Analia V.; Laczeski, Margarita Ester; et al.; Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 2; 10-2018; 842-8461338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2018.8.2.842-846info:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/issue/october-november-2018-vol-8-no-2/jmbfs_418_bobadilla/?issue_id=4902&article_id=16info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:05Zoai:ri.conicet.gov.ar:11336/83936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:06.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
title Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
spellingShingle Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
Bobadilla, Fernando Javier
ANTIBACTERIAL ACTIVITY
ETHANOLIC EXTRACT
EUGENIA UNIFLORA L
PSEUDOMONAS AERUGINOSA
title_short Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
title_full Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
title_fullStr Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
title_full_unstemmed Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
title_sort Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa
dc.creator.none.fl_str_mv Bobadilla, Fernando Javier
Novosak, Marina Gisel
Winnik, Daniana L.
Kachuk, Analia V.
Laczeski, Margarita Ester
Quiroga, Marina I.
author Bobadilla, Fernando Javier
author_facet Bobadilla, Fernando Javier
Novosak, Marina Gisel
Winnik, Daniana L.
Kachuk, Analia V.
Laczeski, Margarita Ester
Quiroga, Marina I.
author_role author
author2 Novosak, Marina Gisel
Winnik, Daniana L.
Kachuk, Analia V.
Laczeski, Margarita Ester
Quiroga, Marina I.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBACTERIAL ACTIVITY
ETHANOLIC EXTRACT
EUGENIA UNIFLORA L
PSEUDOMONAS AERUGINOSA
topic ANTIBACTERIAL ACTIVITY
ETHANOLIC EXTRACT
EUGENIA UNIFLORA L
PSEUDOMONAS AERUGINOSA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pseudomonas aeruginosa is one of the main causes of nosocomial infections worldwide, with great potential for multi-resistance development. The World Health Organization (WHO) considers the antimicrobial resistance as an emerging public health problem that makes new therapies an extremely urgent issue. The aim of this work was to study the antibacterial capacity of the ethanolic extract of Eugenia uniflora L. leaves on Pseudomonas aeruginosa, its possible synergistic action with commercial antibiotics and to carry out general toxicity tests using the crustacean Artemia salina. The extract had a Minimum Inhibitory Dose (MID) of 0.5 mg.disc-1. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) for P. aeruginosa PAO1 were 1.33 mg.ml-1 and 13.33 mg.ml-1. For the clinical isolations were 0.83 mg.ml-1 and 8.33 mg.ml-1. The MBC/MIC ratio (MICI) qualified the action of the extract as bacteriostatic. The Time-killing curve showed that the total loss of viability of the bacteria occurs at 24 h to 16 mg.ml-1 and at 8 h to 32 mg.ml-1. The combination of meropenem with the extract results in a decrease in its inhibitory action on P. aeruginosa PAO1. Meanwhile, the combination with piperacycline or ceftazidime produces a synergistic action. For ciprofloxacin, amicacin and colistin the combination is indifferent. The LC50 obtained using A. salina was 0.61 mg.ml-1; indicating that the extract has a moderate toxicity. These results suggest that this species would be a potential source of antimicrobial agents to be applied in the treatment of infections caused by P. aeruginosa.
Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Winnik, Daniana L.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Kachuk, Analia V.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
Fil: Quiroga, Marina I.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina
description Pseudomonas aeruginosa is one of the main causes of nosocomial infections worldwide, with great potential for multi-resistance development. The World Health Organization (WHO) considers the antimicrobial resistance as an emerging public health problem that makes new therapies an extremely urgent issue. The aim of this work was to study the antibacterial capacity of the ethanolic extract of Eugenia uniflora L. leaves on Pseudomonas aeruginosa, its possible synergistic action with commercial antibiotics and to carry out general toxicity tests using the crustacean Artemia salina. The extract had a Minimum Inhibitory Dose (MID) of 0.5 mg.disc-1. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) for P. aeruginosa PAO1 were 1.33 mg.ml-1 and 13.33 mg.ml-1. For the clinical isolations were 0.83 mg.ml-1 and 8.33 mg.ml-1. The MBC/MIC ratio (MICI) qualified the action of the extract as bacteriostatic. The Time-killing curve showed that the total loss of viability of the bacteria occurs at 24 h to 16 mg.ml-1 and at 8 h to 32 mg.ml-1. The combination of meropenem with the extract results in a decrease in its inhibitory action on P. aeruginosa PAO1. Meanwhile, the combination with piperacycline or ceftazidime produces a synergistic action. For ciprofloxacin, amicacin and colistin the combination is indifferent. The LC50 obtained using A. salina was 0.61 mg.ml-1; indicating that the extract has a moderate toxicity. These results suggest that this species would be a potential source of antimicrobial agents to be applied in the treatment of infections caused by P. aeruginosa.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83936
Bobadilla, Fernando Javier; Novosak, Marina Gisel; Winnik, Daniana L.; Kachuk, Analia V.; Laczeski, Margarita Ester; et al.; Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 2; 10-2018; 842-846
1338-5178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83936
identifier_str_mv Bobadilla, Fernando Javier; Novosak, Marina Gisel; Winnik, Daniana L.; Kachuk, Analia V.; Laczeski, Margarita Ester; et al.; Antibacterial activity and toxicity of the ethanolic extract of Eugenia Uniflora L. Leaves On Pseudomonas Aeruginosa; Slovak University of Agriculture; Journal of Microbiology, Biotechnology and Food Sciences; 8; 2; 10-2018; 842-846
1338-5178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2018.8.2.842-846
info:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/issue/october-november-2018-vol-8-no-2/jmbfs_418_bobadilla/?issue_id=4902&article_id=16
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Slovak University of Agriculture
publisher.none.fl_str_mv Slovak University of Agriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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