Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease

Autores
Amweg, Ayelen Noelia; Salvetti, Natalia Raquel; Stangaferro, M. L.; Paredes, A. H.; Lara, H. H.; Rodríguez, Fernanda Mariel; Ortega, Hugo Hector
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cystic ovarian disease (COD) is an important cause of infertility in cattle, and ACTH has been involved in regulatory mechanisms related to ovarian function associated with ovulation, steroidogenesis, and luteal function. Here, we examined the localization of 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) and 11βHSD2 proteins in the ovary of healthy cows and animals with spontaneous and ACTH-induced COD and the in vitro response of the follicular wall exposed to ACTH. After stimulation by ACTH, we documented changes in 11βHSD expression and cortisol secretion by the follicular wall of large antral and follicular cysts. Follicular cysts showed a higher constitutive expression of both enzymes, whereas ACTH induced an increase in 11βHSD1 in tertiary follicles and follicular cysts and a decrease in 11βHSD2 in follicular cysts. Moderate expression of 11βHSD1 was observed by immunohistochemistry in granulosa of control animals, with an increase (P < 0.05) from primary to secondary, tertiary, and atretic follicles. The level of immunostaining in theca interna was lower than that in granulosa. The expression of 11βHSD2 was lower in the granulosa of primary follicles than in that of secondary, tertiary, and atretic follicles and was lower in the theca interna than in the granulosa. In ACTH-induced and spontaneously occurring follicular cysts, differences from controls were observed only in the expression of 11βHSD1 in the granulosa, being higher (P < 0.05) than in tertiary follicles. These findings indicate that follicular cysts may be exposed to high local concentrations of active glucocorticoids and indicate a local role for cortisol in COD pathogenesis and in regulatory mechanisms of ovarian function.
Fil: Amweg, Ayelen Noelia. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Stangaferro, M. L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Paredes, A. H.. Universidad de Chile; Chile
Fil: Lara, H. H.. Universidad de Chile; Chile
Fil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Acth
Cystic Ovarian Disease
11beta Hsd
Bovine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6407
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6407
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian diseaseAmweg, Ayelen NoeliaSalvetti, Natalia RaquelStangaferro, M. L.Paredes, A. H.Lara, H. H.Rodríguez, Fernanda MarielOrtega, Hugo HectorActhCystic Ovarian Disease11beta HsdBovinehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Cystic ovarian disease (COD) is an important cause of infertility in cattle, and ACTH has been involved in regulatory mechanisms related to ovarian function associated with ovulation, steroidogenesis, and luteal function. Here, we examined the localization of 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) and 11βHSD2 proteins in the ovary of healthy cows and animals with spontaneous and ACTH-induced COD and the in vitro response of the follicular wall exposed to ACTH. After stimulation by ACTH, we documented changes in 11βHSD expression and cortisol secretion by the follicular wall of large antral and follicular cysts. Follicular cysts showed a higher constitutive expression of both enzymes, whereas ACTH induced an increase in 11βHSD1 in tertiary follicles and follicular cysts and a decrease in 11βHSD2 in follicular cysts. Moderate expression of 11βHSD1 was observed by immunohistochemistry in granulosa of control animals, with an increase (P < 0.05) from primary to secondary, tertiary, and atretic follicles. The level of immunostaining in theca interna was lower than that in granulosa. The expression of 11βHSD2 was lower in the granulosa of primary follicles than in that of secondary, tertiary, and atretic follicles and was lower in the theca interna than in the granulosa. In ACTH-induced and spontaneously occurring follicular cysts, differences from controls were observed only in the expression of 11βHSD1 in the granulosa, being higher (P < 0.05) than in tertiary follicles. These findings indicate that follicular cysts may be exposed to high local concentrations of active glucocorticoids and indicate a local role for cortisol in COD pathogenesis and in regulatory mechanisms of ovarian function.Fil: Amweg, Ayelen Noelia. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Stangaferro, M. L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Paredes, A. H.. Universidad de Chile; ChileFil: Lara, H. H.. Universidad de Chile; ChileFil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevier2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6407Amweg, Ayelen Noelia; Salvetti, Natalia Raquel; Stangaferro, M. L.; Paredes, A. H.; Lara, H. H.; et al.; Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease; Elsevier; Domestic Animal Endocrinology; 45; 3; 10-2013; 126-1400739-7240enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.domaniend.2013.07.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:34Zoai:ri.conicet.gov.ar:11336/6407instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:34.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
title Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
spellingShingle Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
Amweg, Ayelen Noelia
Acth
Cystic Ovarian Disease
11beta Hsd
Bovine
title_short Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
title_full Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
title_fullStr Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
title_full_unstemmed Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
title_sort Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease
dc.creator.none.fl_str_mv Amweg, Ayelen Noelia
Salvetti, Natalia Raquel
Stangaferro, M. L.
Paredes, A. H.
Lara, H. H.
Rodríguez, Fernanda Mariel
Ortega, Hugo Hector
author Amweg, Ayelen Noelia
author_facet Amweg, Ayelen Noelia
Salvetti, Natalia Raquel
Stangaferro, M. L.
Paredes, A. H.
Lara, H. H.
Rodríguez, Fernanda Mariel
Ortega, Hugo Hector
author_role author
author2 Salvetti, Natalia Raquel
Stangaferro, M. L.
Paredes, A. H.
Lara, H. H.
Rodríguez, Fernanda Mariel
Ortega, Hugo Hector
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acth
Cystic Ovarian Disease
11beta Hsd
Bovine
topic Acth
Cystic Ovarian Disease
11beta Hsd
Bovine
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cystic ovarian disease (COD) is an important cause of infertility in cattle, and ACTH has been involved in regulatory mechanisms related to ovarian function associated with ovulation, steroidogenesis, and luteal function. Here, we examined the localization of 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) and 11βHSD2 proteins in the ovary of healthy cows and animals with spontaneous and ACTH-induced COD and the in vitro response of the follicular wall exposed to ACTH. After stimulation by ACTH, we documented changes in 11βHSD expression and cortisol secretion by the follicular wall of large antral and follicular cysts. Follicular cysts showed a higher constitutive expression of both enzymes, whereas ACTH induced an increase in 11βHSD1 in tertiary follicles and follicular cysts and a decrease in 11βHSD2 in follicular cysts. Moderate expression of 11βHSD1 was observed by immunohistochemistry in granulosa of control animals, with an increase (P < 0.05) from primary to secondary, tertiary, and atretic follicles. The level of immunostaining in theca interna was lower than that in granulosa. The expression of 11βHSD2 was lower in the granulosa of primary follicles than in that of secondary, tertiary, and atretic follicles and was lower in the theca interna than in the granulosa. In ACTH-induced and spontaneously occurring follicular cysts, differences from controls were observed only in the expression of 11βHSD1 in the granulosa, being higher (P < 0.05) than in tertiary follicles. These findings indicate that follicular cysts may be exposed to high local concentrations of active glucocorticoids and indicate a local role for cortisol in COD pathogenesis and in regulatory mechanisms of ovarian function.
Fil: Amweg, Ayelen Noelia. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Stangaferro, M. L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Paredes, A. H.. Universidad de Chile; Chile
Fil: Lara, H. H.. Universidad de Chile; Chile
Fil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Departamento de Cs. Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Cystic ovarian disease (COD) is an important cause of infertility in cattle, and ACTH has been involved in regulatory mechanisms related to ovarian function associated with ovulation, steroidogenesis, and luteal function. Here, we examined the localization of 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) and 11βHSD2 proteins in the ovary of healthy cows and animals with spontaneous and ACTH-induced COD and the in vitro response of the follicular wall exposed to ACTH. After stimulation by ACTH, we documented changes in 11βHSD expression and cortisol secretion by the follicular wall of large antral and follicular cysts. Follicular cysts showed a higher constitutive expression of both enzymes, whereas ACTH induced an increase in 11βHSD1 in tertiary follicles and follicular cysts and a decrease in 11βHSD2 in follicular cysts. Moderate expression of 11βHSD1 was observed by immunohistochemistry in granulosa of control animals, with an increase (P < 0.05) from primary to secondary, tertiary, and atretic follicles. The level of immunostaining in theca interna was lower than that in granulosa. The expression of 11βHSD2 was lower in the granulosa of primary follicles than in that of secondary, tertiary, and atretic follicles and was lower in the theca interna than in the granulosa. In ACTH-induced and spontaneously occurring follicular cysts, differences from controls were observed only in the expression of 11βHSD1 in the granulosa, being higher (P < 0.05) than in tertiary follicles. These findings indicate that follicular cysts may be exposed to high local concentrations of active glucocorticoids and indicate a local role for cortisol in COD pathogenesis and in regulatory mechanisms of ovarian function.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6407
Amweg, Ayelen Noelia; Salvetti, Natalia Raquel; Stangaferro, M. L.; Paredes, A. H.; Lara, H. H.; et al.; Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease; Elsevier; Domestic Animal Endocrinology; 45; 3; 10-2013; 126-140
0739-7240
url http://hdl.handle.net/11336/6407
identifier_str_mv Amweg, Ayelen Noelia; Salvetti, Natalia Raquel; Stangaferro, M. L.; Paredes, A. H.; Lara, H. H.; et al.; Ovarian localization of 11b-hydroxysteroid dehydrogenase (11bHSD): effects of ACTH stimulation and its relationship with bovine cystic ovarian disease; Elsevier; Domestic Animal Endocrinology; 45; 3; 10-2013; 126-140
0739-7240
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.domaniend.2013.07.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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