Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease

Autores
Lucero, María Soledad; Gómez, Evangelina Raquel; Richetta, Matías Daniel; Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; Gravisaco, Maria Jose Federica; Delgado, Fernando Oscar; Berinstein, Analia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry. The structural protein VP2 has been used for the development of subunit vaccines in a variety of heterologous platforms. We have previously demonstrated that plant-derived VP2 (pVP2) is able to elicit a neutralizing antibody response in chickens when administered intramuscularly (i.m.) in a prime/boost scheme. However, administration via injection is impractical and carries the risk of needle stick injury or pain. Mucosal vaccination is noninvasive and has several advantages over traditional systemic vaccines. Taking this into account and the fact that natural infections with IBDV occur by the oral route, we decided to investigate whether pVP2 was also immunogenic when given intranasally (i.n.) or orally to chickens. In addition, we evaluated if intramuscular vaccination with VP2 plant extract in a more welfarefriendly scheme with less injections and without adjuvant was able to elicit a protective immune response against IBDV as previously seen. We determined that animals inoculated i.m., but not i.n., with the experimental vaccine developed high titres of specific antibodies, with virus neutralizing activity. Also, bursae of animals vaccinated i.m. with pVP2 presented few infiltrating T cells, low viral charge and normal morphology. However, chickens that received the immunogen via nasal or oral route were not protected after challenge. Considering the disadvantages of conventional live-attenuated and inactivated vaccines, a plant-based subunit vaccine represents a viable alternative in the veterinary field. Once again pVP2 has proven to be immunogenic when parentally inoculated. However, further investigations need to be done in order to find an alternative route of administration which is more practical than the intramuscular injection and capable of eliciting a mucosal immune response.
Fil: Lucero, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Gómez, Evangelina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Richetta, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Chimeno Zoth, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Carballeda, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, Maria Jose Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Berinstein, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
INFECTIOUS BURSAL DISEASE
VP2
PLANT BASED VACCINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106911
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/106911
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal diseaseLucero, María SoledadGómez, Evangelina RaquelRichetta, Matías DanielChimeno Zoth, Silvina AndreaCarballeda, Juan ManuelGravisaco, Maria Jose FedericaDelgado, Fernando OscarBerinstein, AnaliaINFECTIOUS BURSAL DISEASEVP2PLANT BASED VACCINEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry. The structural protein VP2 has been used for the development of subunit vaccines in a variety of heterologous platforms. We have previously demonstrated that plant-derived VP2 (pVP2) is able to elicit a neutralizing antibody response in chickens when administered intramuscularly (i.m.) in a prime/boost scheme. However, administration via injection is impractical and carries the risk of needle stick injury or pain. Mucosal vaccination is noninvasive and has several advantages over traditional systemic vaccines. Taking this into account and the fact that natural infections with IBDV occur by the oral route, we decided to investigate whether pVP2 was also immunogenic when given intranasally (i.n.) or orally to chickens. In addition, we evaluated if intramuscular vaccination with VP2 plant extract in a more welfarefriendly scheme with less injections and without adjuvant was able to elicit a protective immune response against IBDV as previously seen. We determined that animals inoculated i.m., but not i.n., with the experimental vaccine developed high titres of specific antibodies, with virus neutralizing activity. Also, bursae of animals vaccinated i.m. with pVP2 presented few infiltrating T cells, low viral charge and normal morphology. However, chickens that received the immunogen via nasal or oral route were not protected after challenge. Considering the disadvantages of conventional live-attenuated and inactivated vaccines, a plant-based subunit vaccine represents a viable alternative in the veterinary field. Once again pVP2 has proven to be immunogenic when parentally inoculated. However, further investigations need to be done in order to find an alternative route of administration which is more practical than the intramuscular injection and capable of eliciting a mucosal immune response.Fil: Lucero, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Gómez, Evangelina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Richetta, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Chimeno Zoth, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Carballeda, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gravisaco, Maria Jose Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Berinstein, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaSciTechnol2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106911Lucero, María Soledad; Gómez, Evangelina Raquel; Richetta, Matías Daniel; Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; et al.; Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease; SciTechnol; Journal of Virology & Antiviral Research; 5; 4; 9-2016; 1-72324-8955CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8955.1000161info:eu-repo/semantics/altIdentifier/url/https://www.scitechnol.com/peer-review/effect-of-different-routes-of-inoculation-on-plantderived-vp2-immunogenicity-and-ability-to-confer-protection-against-infectious-b-5XrH.php?article_id=5327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:39Zoai:ri.conicet.gov.ar:11336/106911instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:39.786CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
title Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
spellingShingle Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
Lucero, María Soledad
INFECTIOUS BURSAL DISEASE
VP2
PLANT BASED VACCINE
title_short Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
title_full Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
title_fullStr Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
title_full_unstemmed Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
title_sort Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease
dc.creator.none.fl_str_mv Lucero, María Soledad
Gómez, Evangelina Raquel
Richetta, Matías Daniel
Chimeno Zoth, Silvina Andrea
Carballeda, Juan Manuel
Gravisaco, Maria Jose Federica
Delgado, Fernando Oscar
Berinstein, Analia
author Lucero, María Soledad
author_facet Lucero, María Soledad
Gómez, Evangelina Raquel
Richetta, Matías Daniel
Chimeno Zoth, Silvina Andrea
Carballeda, Juan Manuel
Gravisaco, Maria Jose Federica
Delgado, Fernando Oscar
Berinstein, Analia
author_role author
author2 Gómez, Evangelina Raquel
Richetta, Matías Daniel
Chimeno Zoth, Silvina Andrea
Carballeda, Juan Manuel
Gravisaco, Maria Jose Federica
Delgado, Fernando Oscar
Berinstein, Analia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFECTIOUS BURSAL DISEASE
VP2
PLANT BASED VACCINE
topic INFECTIOUS BURSAL DISEASE
VP2
PLANT BASED VACCINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry. The structural protein VP2 has been used for the development of subunit vaccines in a variety of heterologous platforms. We have previously demonstrated that plant-derived VP2 (pVP2) is able to elicit a neutralizing antibody response in chickens when administered intramuscularly (i.m.) in a prime/boost scheme. However, administration via injection is impractical and carries the risk of needle stick injury or pain. Mucosal vaccination is noninvasive and has several advantages over traditional systemic vaccines. Taking this into account and the fact that natural infections with IBDV occur by the oral route, we decided to investigate whether pVP2 was also immunogenic when given intranasally (i.n.) or orally to chickens. In addition, we evaluated if intramuscular vaccination with VP2 plant extract in a more welfarefriendly scheme with less injections and without adjuvant was able to elicit a protective immune response against IBDV as previously seen. We determined that animals inoculated i.m., but not i.n., with the experimental vaccine developed high titres of specific antibodies, with virus neutralizing activity. Also, bursae of animals vaccinated i.m. with pVP2 presented few infiltrating T cells, low viral charge and normal morphology. However, chickens that received the immunogen via nasal or oral route were not protected after challenge. Considering the disadvantages of conventional live-attenuated and inactivated vaccines, a plant-based subunit vaccine represents a viable alternative in the veterinary field. Once again pVP2 has proven to be immunogenic when parentally inoculated. However, further investigations need to be done in order to find an alternative route of administration which is more practical than the intramuscular injection and capable of eliciting a mucosal immune response.
Fil: Lucero, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Gómez, Evangelina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Richetta, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Chimeno Zoth, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Carballeda, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, Maria Jose Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Berinstein, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry. The structural protein VP2 has been used for the development of subunit vaccines in a variety of heterologous platforms. We have previously demonstrated that plant-derived VP2 (pVP2) is able to elicit a neutralizing antibody response in chickens when administered intramuscularly (i.m.) in a prime/boost scheme. However, administration via injection is impractical and carries the risk of needle stick injury or pain. Mucosal vaccination is noninvasive and has several advantages over traditional systemic vaccines. Taking this into account and the fact that natural infections with IBDV occur by the oral route, we decided to investigate whether pVP2 was also immunogenic when given intranasally (i.n.) or orally to chickens. In addition, we evaluated if intramuscular vaccination with VP2 plant extract in a more welfarefriendly scheme with less injections and without adjuvant was able to elicit a protective immune response against IBDV as previously seen. We determined that animals inoculated i.m., but not i.n., with the experimental vaccine developed high titres of specific antibodies, with virus neutralizing activity. Also, bursae of animals vaccinated i.m. with pVP2 presented few infiltrating T cells, low viral charge and normal morphology. However, chickens that received the immunogen via nasal or oral route were not protected after challenge. Considering the disadvantages of conventional live-attenuated and inactivated vaccines, a plant-based subunit vaccine represents a viable alternative in the veterinary field. Once again pVP2 has proven to be immunogenic when parentally inoculated. However, further investigations need to be done in order to find an alternative route of administration which is more practical than the intramuscular injection and capable of eliciting a mucosal immune response.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106911
Lucero, María Soledad; Gómez, Evangelina Raquel; Richetta, Matías Daniel; Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; et al.; Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease; SciTechnol; Journal of Virology & Antiviral Research; 5; 4; 9-2016; 1-7
2324-8955
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106911
identifier_str_mv Lucero, María Soledad; Gómez, Evangelina Raquel; Richetta, Matías Daniel; Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; et al.; Effect of different routes of inoculation on plant-derived VP2 immunogenicity and ability to confer protection against infectious bursal disease; SciTechnol; Journal of Virology & Antiviral Research; 5; 4; 9-2016; 1-7
2324-8955
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8955.1000161
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv SciTechnol
publisher.none.fl_str_mv SciTechnol
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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