Effect of laundry detergent formulation on the performance of alkaline phytoproteases

Autores
Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.
Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; Argentina
Fil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; Argentina
Fil: Barcia, Cristina. Universidad Nacional de San Luis; Argentina
Fil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; Argentina
Materia
asclepain
araujain
detergents formulations
alkaline plant proteases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1005
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1005
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of laundry detergent formulation on the performance of alkaline phytoproteasesBarberis, Sonia EstherQuiroga, EvelinaBarcia, CristinaLiggiere, Constanzaasclepainaraujaindetergents formulationsalkaline plant proteaseshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; ArgentinaFil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; ArgentinaFil: Barcia, Cristina. Universidad Nacional de San Luis; ArgentinaFil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; ArgentinaUniv Catolica de Valparaiso2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1005Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-80717-3458enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/indexAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:47Zoai:ri.conicet.gov.ar:11336/1005instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:47.694CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of laundry detergent formulation on the performance of alkaline phytoproteases
title Effect of laundry detergent formulation on the performance of alkaline phytoproteases
spellingShingle Effect of laundry detergent formulation on the performance of alkaline phytoproteases
Barberis, Sonia Esther
asclepain
araujain
detergents formulations
alkaline plant proteases
title_short Effect of laundry detergent formulation on the performance of alkaline phytoproteases
title_full Effect of laundry detergent formulation on the performance of alkaline phytoproteases
title_fullStr Effect of laundry detergent formulation on the performance of alkaline phytoproteases
title_full_unstemmed Effect of laundry detergent formulation on the performance of alkaline phytoproteases
title_sort Effect of laundry detergent formulation on the performance of alkaline phytoproteases
dc.creator.none.fl_str_mv Barberis, Sonia Esther
Quiroga, Evelina
Barcia, Cristina
Liggiere, Constanza
author Barberis, Sonia Esther
author_facet Barberis, Sonia Esther
Quiroga, Evelina
Barcia, Cristina
Liggiere, Constanza
author_role author
author2 Quiroga, Evelina
Barcia, Cristina
Liggiere, Constanza
author2_role author
author
author
dc.subject.none.fl_str_mv asclepain
araujain
detergents formulations
alkaline plant proteases
topic asclepain
araujain
detergents formulations
alkaline plant proteases
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.
Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; Argentina
Fil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; Argentina
Fil: Barcia, Cristina. Universidad Nacional de San Luis; Argentina
Fil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; Argentina
description Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1005
Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-8
0717-3458
url http://hdl.handle.net/11336/1005
identifier_str_mv Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-8
0717-3458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003
info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/index
dc.rights.none.fl_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Catolica de Valparaiso
publisher.none.fl_str_mv Univ Catolica de Valparaiso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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