Effect of laundry detergent formulation on the performance of alkaline phytoproteases
- Autores
- Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.
Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; Argentina
Fil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; Argentina
Fil: Barcia, Cristina. Universidad Nacional de San Luis; Argentina
Fil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; Argentina - Materia
-
asclepain
araujain
detergents formulations
alkaline plant proteases - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1005
Ver los metadatos del registro completo
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Effect of laundry detergent formulation on the performance of alkaline phytoproteasesBarberis, Sonia EstherQuiroga, EvelinaBarcia, CristinaLiggiere, Constanzaasclepainaraujaindetergents formulationsalkaline plant proteaseshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; ArgentinaFil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; ArgentinaFil: Barcia, Cristina. Universidad Nacional de San Luis; ArgentinaFil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; ArgentinaUniv Catolica de Valparaiso2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1005Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-80717-3458enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/indexAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:26Zoai:ri.conicet.gov.ar:11336/1005instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:26.47CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
title |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
spellingShingle |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases Barberis, Sonia Esther asclepain araujain detergents formulations alkaline plant proteases |
title_short |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
title_full |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
title_fullStr |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
title_full_unstemmed |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
title_sort |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
dc.creator.none.fl_str_mv |
Barberis, Sonia Esther Quiroga, Evelina Barcia, Cristina Liggiere, Constanza |
author |
Barberis, Sonia Esther |
author_facet |
Barberis, Sonia Esther Quiroga, Evelina Barcia, Cristina Liggiere, Constanza |
author_role |
author |
author2 |
Quiroga, Evelina Barcia, Cristina Liggiere, Constanza |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
asclepain araujain detergents formulations alkaline plant proteases |
topic |
asclepain araujain detergents formulations alkaline plant proteases |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain. Fil: Barberis, Sonia Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatologia; Argentina Fil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- San Luis. Instituto de Física Aplicada; Argentina Fil: Barcia, Cristina. Universidad Nacional de San Luis; Argentina Fil: Liggiere, Constanza. Universidad Nacional de la Plata. Facultad de Ciencias Exactas; Argentina |
description |
Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1005 Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-8 0717-3458 |
url |
http://hdl.handle.net/11336/1005 |
identifier_str_mv |
Barberis, Sonia Esther; Quiroga, Evelina; Barcia, Cristina; Liggiere, Constanza; Effect of laundry detergent formulation on the performance of alkaline phytoproteases; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 3; 5-2013; 1-8 0717-3458 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003 info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/index |
dc.rights.none.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
rights_invalid_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Univ Catolica de Valparaiso |
publisher.none.fl_str_mv |
Univ Catolica de Valparaiso |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614287997272064 |
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13.070432 |