Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments

Autores
Cadavid Vargas, Juan Fernando; Larrañaga, Aitor; Lizundia, Erlantz
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among nanoscale biopolymers, fungal chitin nanofibrils (ChNFs) stand out for their low carbon footprint and functional properties. However, the nanotoxicity properties of ChNFs have not been fully elucidated. To fill this knowledge gap, here we investigate the cytotoxicity and inflammatory effects of chemically modified ChNFs having gradient acid and alkaline treatments. ChNFs isolated from white mushroom exhibit a long fibrous morphology with diameters of 3–8 nm and lengths of 150–600 nm, and are composed of α-chitin in coexistence with amorphous covalently bounded β-glucans. Both alkaline (2 m NaOH) and acidic (2 m HCl) treatments impact the crystallinity, N-acetylation, zeta potential, and nitrogen content values to provide ranges of 23-to-51 %, 45-to-99 %, −5 to +26 mV, and 2.6-to-5.2 %, respectively. Nanotoxicity studies with colloidal dispersions demonstrate differences in the inflammatory response by cells after chemical post-treatments. The NaOH-treated ChNFs elicited a much lower inflammatory response, attenuating the release of nitrites and the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Alginate hydrogels with ChNFs were further fabricated and demonstrated potential to host cells in three-dimensional microenvironments, preserving a good metabolic activity, viability, and cell proliferation. These results may guide new applications of fungal nanochitin in pharmaceutical or tissue engineering.
Fil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Larrañaga, Aitor. Universidad del País Vasco; España
Fil: Lizundia, Erlantz. Universidad del País Vasco; España
Materia
nanochitin
inflammation
microglia
mushroom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281441
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281441
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatmentsCadavid Vargas, Juan FernandoLarrañaga, AitorLizundia, Erlantznanochitininflammationmicrogliamushroomhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Among nanoscale biopolymers, fungal chitin nanofibrils (ChNFs) stand out for their low carbon footprint and functional properties. However, the nanotoxicity properties of ChNFs have not been fully elucidated. To fill this knowledge gap, here we investigate the cytotoxicity and inflammatory effects of chemically modified ChNFs having gradient acid and alkaline treatments. ChNFs isolated from white mushroom exhibit a long fibrous morphology with diameters of 3–8 nm and lengths of 150–600 nm, and are composed of α-chitin in coexistence with amorphous covalently bounded β-glucans. Both alkaline (2 m NaOH) and acidic (2 m HCl) treatments impact the crystallinity, N-acetylation, zeta potential, and nitrogen content values to provide ranges of 23-to-51 %, 45-to-99 %, −5 to +26 mV, and 2.6-to-5.2 %, respectively. Nanotoxicity studies with colloidal dispersions demonstrate differences in the inflammatory response by cells after chemical post-treatments. The NaOH-treated ChNFs elicited a much lower inflammatory response, attenuating the release of nitrites and the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Alginate hydrogels with ChNFs were further fabricated and demonstrated potential to host cells in three-dimensional microenvironments, preserving a good metabolic activity, viability, and cell proliferation. These results may guide new applications of fungal nanochitin in pharmaceutical or tissue engineering.Fil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Larrañaga, Aitor. Universidad del País Vasco; EspañaFil: Lizundia, Erlantz. Universidad del País Vasco; EspañaElsevier2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281441Cadavid Vargas, Juan Fernando; Larrañaga, Aitor; Lizundia, Erlantz; Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments; Elsevier; Carbohydrate Polymers; 357; 6-2025; 1-120144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0144861725002656info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2025.123484info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:27:02Zoai:ri.conicet.gov.ar:11336/281441instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:27:02.961CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
title Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
spellingShingle Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
Cadavid Vargas, Juan Fernando
nanochitin
inflammation
microglia
mushroom
title_short Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
title_full Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
title_fullStr Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
title_full_unstemmed Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
title_sort Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments
dc.creator.none.fl_str_mv Cadavid Vargas, Juan Fernando
Larrañaga, Aitor
Lizundia, Erlantz
author Cadavid Vargas, Juan Fernando
author_facet Cadavid Vargas, Juan Fernando
Larrañaga, Aitor
Lizundia, Erlantz
author_role author
author2 Larrañaga, Aitor
Lizundia, Erlantz
author2_role author
author
dc.subject.none.fl_str_mv nanochitin
inflammation
microglia
mushroom
topic nanochitin
inflammation
microglia
mushroom
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Among nanoscale biopolymers, fungal chitin nanofibrils (ChNFs) stand out for their low carbon footprint and functional properties. However, the nanotoxicity properties of ChNFs have not been fully elucidated. To fill this knowledge gap, here we investigate the cytotoxicity and inflammatory effects of chemically modified ChNFs having gradient acid and alkaline treatments. ChNFs isolated from white mushroom exhibit a long fibrous morphology with diameters of 3–8 nm and lengths of 150–600 nm, and are composed of α-chitin in coexistence with amorphous covalently bounded β-glucans. Both alkaline (2 m NaOH) and acidic (2 m HCl) treatments impact the crystallinity, N-acetylation, zeta potential, and nitrogen content values to provide ranges of 23-to-51 %, 45-to-99 %, −5 to +26 mV, and 2.6-to-5.2 %, respectively. Nanotoxicity studies with colloidal dispersions demonstrate differences in the inflammatory response by cells after chemical post-treatments. The NaOH-treated ChNFs elicited a much lower inflammatory response, attenuating the release of nitrites and the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Alginate hydrogels with ChNFs were further fabricated and demonstrated potential to host cells in three-dimensional microenvironments, preserving a good metabolic activity, viability, and cell proliferation. These results may guide new applications of fungal nanochitin in pharmaceutical or tissue engineering.
Fil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Larrañaga, Aitor. Universidad del País Vasco; España
Fil: Lizundia, Erlantz. Universidad del País Vasco; España
description Among nanoscale biopolymers, fungal chitin nanofibrils (ChNFs) stand out for their low carbon footprint and functional properties. However, the nanotoxicity properties of ChNFs have not been fully elucidated. To fill this knowledge gap, here we investigate the cytotoxicity and inflammatory effects of chemically modified ChNFs having gradient acid and alkaline treatments. ChNFs isolated from white mushroom exhibit a long fibrous morphology with diameters of 3–8 nm and lengths of 150–600 nm, and are composed of α-chitin in coexistence with amorphous covalently bounded β-glucans. Both alkaline (2 m NaOH) and acidic (2 m HCl) treatments impact the crystallinity, N-acetylation, zeta potential, and nitrogen content values to provide ranges of 23-to-51 %, 45-to-99 %, −5 to +26 mV, and 2.6-to-5.2 %, respectively. Nanotoxicity studies with colloidal dispersions demonstrate differences in the inflammatory response by cells after chemical post-treatments. The NaOH-treated ChNFs elicited a much lower inflammatory response, attenuating the release of nitrites and the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Alginate hydrogels with ChNFs were further fabricated and demonstrated potential to host cells in three-dimensional microenvironments, preserving a good metabolic activity, viability, and cell proliferation. These results may guide new applications of fungal nanochitin in pharmaceutical or tissue engineering.
publishDate 2025
dc.date.none.fl_str_mv 2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281441
Cadavid Vargas, Juan Fernando; Larrañaga, Aitor; Lizundia, Erlantz; Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments; Elsevier; Carbohydrate Polymers; 357; 6-2025; 1-12
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281441
identifier_str_mv Cadavid Vargas, Juan Fernando; Larrañaga, Aitor; Lizundia, Erlantz; Structure and nanotoxicity of fungal chitin-glucan nanofibrils with gradient acid and alkaline treatments; Elsevier; Carbohydrate Polymers; 357; 6-2025; 1-12
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0144861725002656
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2025.123484
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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