Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
- Autores
- Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; Quiberoni, Andrea del Lujan
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Deveau, H.. Laval University; Canadá
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Moineau, S.. Laval University; Canadá
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina - Materia
-
STREPTOCOCCUS THERMOPHILUS
BACTERIOPHAGE
SEQUENCE ANALYSIS
STRUCTURAL PROTEINS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/103372
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/103372 |
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3498 |
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CONICET Digital (CONICET) |
spelling |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in ArgentinaGuglielmotti, Daniela MartaDeveau, H.Binetti, Ana GriseldaReinheimer, Jorge AlbertoMoineau, S.Quiberoni, Andrea del LujanSTREPTOCOCCUS THERMOPHILUSBACTERIOPHAGESEQUENCE ANALYSISSTRUCTURAL PROTEINShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Deveau, H.. Laval University; CanadáFil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Moineau, S.. Laval University; CanadáFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaElsevier Science2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103372Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-1090168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2009.09.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:39Zoai:ri.conicet.gov.ar:11336/103372instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:39.891CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
title |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
spellingShingle |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina Guglielmotti, Daniela Marta STREPTOCOCCUS THERMOPHILUS BACTERIOPHAGE SEQUENCE ANALYSIS STRUCTURAL PROTEINS |
title_short |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
title_full |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
title_fullStr |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
title_full_unstemmed |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
title_sort |
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina |
dc.creator.none.fl_str_mv |
Guglielmotti, Daniela Marta Deveau, H. Binetti, Ana Griselda Reinheimer, Jorge Alberto Moineau, S. Quiberoni, Andrea del Lujan |
author |
Guglielmotti, Daniela Marta |
author_facet |
Guglielmotti, Daniela Marta Deveau, H. Binetti, Ana Griselda Reinheimer, Jorge Alberto Moineau, S. Quiberoni, Andrea del Lujan |
author_role |
author |
author2 |
Deveau, H. Binetti, Ana Griselda Reinheimer, Jorge Alberto Moineau, S. Quiberoni, Andrea del Lujan |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
STREPTOCOCCUS THERMOPHILUS BACTERIOPHAGE SEQUENCE ANALYSIS STRUCTURAL PROTEINS |
topic |
STREPTOCOCCUS THERMOPHILUS BACTERIOPHAGE SEQUENCE ANALYSIS STRUCTURAL PROTEINS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures. Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Deveau, H.. Laval University; Canadá Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Moineau, S.. Laval University; Canadá Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina |
description |
Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/103372 Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-109 0168-1605 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/103372 |
identifier_str_mv |
Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-109 0168-1605 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2009.09.005 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613636993056768 |
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13.070432 |