Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina

Autores
Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; Quiberoni, Andrea del Lujan
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Deveau, H.. Laval University; Canadá
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Moineau, S.. Laval University; Canadá
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
STREPTOCOCCUS THERMOPHILUS
BACTERIOPHAGE
SEQUENCE ANALYSIS
STRUCTURAL PROTEINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103372
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103372
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome analysis of two virulent Streptococcus thermophilus phages isolated in ArgentinaGuglielmotti, Daniela MartaDeveau, H.Binetti, Ana GriseldaReinheimer, Jorge AlbertoMoineau, S.Quiberoni, Andrea del LujanSTREPTOCOCCUS THERMOPHILUSBACTERIOPHAGESEQUENCE ANALYSISSTRUCTURAL PROTEINShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Deveau, H.. Laval University; CanadáFil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Moineau, S.. Laval University; CanadáFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaElsevier Science2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103372Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-1090168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2009.09.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:59Zoai:ri.conicet.gov.ar:11336/103372instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:59.845CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
title Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
spellingShingle Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
Guglielmotti, Daniela Marta
STREPTOCOCCUS THERMOPHILUS
BACTERIOPHAGE
SEQUENCE ANALYSIS
STRUCTURAL PROTEINS
title_short Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
title_full Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
title_fullStr Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
title_full_unstemmed Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
title_sort Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
dc.creator.none.fl_str_mv Guglielmotti, Daniela Marta
Deveau, H.
Binetti, Ana Griselda
Reinheimer, Jorge Alberto
Moineau, S.
Quiberoni, Andrea del Lujan
author Guglielmotti, Daniela Marta
author_facet Guglielmotti, Daniela Marta
Deveau, H.
Binetti, Ana Griselda
Reinheimer, Jorge Alberto
Moineau, S.
Quiberoni, Andrea del Lujan
author_role author
author2 Deveau, H.
Binetti, Ana Griselda
Reinheimer, Jorge Alberto
Moineau, S.
Quiberoni, Andrea del Lujan
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv STREPTOCOCCUS THERMOPHILUS
BACTERIOPHAGE
SEQUENCE ANALYSIS
STRUCTURAL PROTEINS
topic STREPTOCOCCUS THERMOPHILUS
BACTERIOPHAGE
SEQUENCE ANALYSIS
STRUCTURAL PROTEINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Deveau, H.. Laval University; Canadá
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Moineau, S.. Laval University; Canadá
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Two Streptococcus thermophilus phages (ALQ13.2 and Abc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525 bp in length for the pac-type phage ALQ13.2 and 34,882 bp for the cos-type phage Abc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and Abc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the Abc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDSPAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of Abc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103372
Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-109
0168-1605
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103372
identifier_str_mv Guglielmotti, Daniela Marta; Deveau, H.; Binetti, Ana Griselda; Reinheimer, Jorge Alberto; Moineau, S.; et al.; Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina; Elsevier Science; International Journal of Food Microbiology; 136; 1; 11-2009; 101-109
0168-1605
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2009.09.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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