Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons

Autores
Gómez Padilla, Eurídice; Bello Medina, Paola C.; León Jacinto, Uriel; Orta Salazar, Erika; Quirarte, Gina L.; Ramirez Amaya, Victor; Prado Alcalá, Roberto A.; Díaz Cintra, Sofía
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The hippocampus plays a fundamental role in spatial learning and memory. Dentate gyrus (DG) granular neurons project mainly to proximal apical dendrites of neurons in the CA3 stratum lucidum and also, to some extent, to the basal dendrites of CA3 pyramidal cells in the stratum oriens. The terminal specializations of DG neurons are the mossy fibers (MF), and these huge axon terminals show expansion in the CA3 stratum oriens after the animals undergo overtraining in the Morris Water Maze task (MWM). However, to our knowledge there are no reports regarding the possible changes in density of post-synaptic targets of these terminals in the basal dendrites of CA3 neurons after overtraining in the MWM. The purpose of this work was to study the density of thorny excrescences (TE) and other dendritic spine types (stubby, thin, and mushroom) in the CA3 stratum oriens in animals overtrained in the MWM for three consecutive days and in animals trained for only one day. Seven days after MWM training, the animals were sacrificed, and their brains removed and processed for rapid Golgi staining to visualize the different types of dendritic protrusions. Our results revealed that the relative quantity of stubby, thin, and mushroom dendritic spines did not change, regardless of amount of training. However, a significant increase in the density of TE was detected in the overtrained animals. These results strongly suggest that spatial water maze overtraining induces an increased density of MF–TE connections, which might be functionally relevant for long-term spatial memory formation.
Fil: Gómez Padilla, Eurídice. Universidad Autonoma de Queretaro.; México
Fil: Bello Medina, Paola C.. Universidad Nacional Autónoma de México; México
Fil: León Jacinto, Uriel. Universidad Nacional Autónoma de México; México
Fil: Orta Salazar, Erika. Universidad Nacional Autónoma de México; México
Fil: Quirarte, Gina L.. Universidad Nacional Autónoma de México; México
Fil: Ramirez Amaya, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Autonoma de Queretaro.; México
Fil: Prado Alcalá, Roberto A.. Universidad Nacional Autónoma de México; México
Fil: Díaz Cintra, Sofía. Universidad Nacional Autónoma de México; México
Materia
CA3
DENDRITIC SPINES
HIPPOCAMPUS
MORRIS WATER MAZE
OVERTRAINING
THORNY EXCRESCENCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140203
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/140203
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neuronsGómez Padilla, EurídiceBello Medina, Paola C.León Jacinto, UrielOrta Salazar, ErikaQuirarte, Gina L.Ramirez Amaya, VictorPrado Alcalá, Roberto A.Díaz Cintra, SofíaCA3DENDRITIC SPINESHIPPOCAMPUSMORRIS WATER MAZEOVERTRAININGTHORNY EXCRESCENCEShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1The hippocampus plays a fundamental role in spatial learning and memory. Dentate gyrus (DG) granular neurons project mainly to proximal apical dendrites of neurons in the CA3 stratum lucidum and also, to some extent, to the basal dendrites of CA3 pyramidal cells in the stratum oriens. The terminal specializations of DG neurons are the mossy fibers (MF), and these huge axon terminals show expansion in the CA3 stratum oriens after the animals undergo overtraining in the Morris Water Maze task (MWM). However, to our knowledge there are no reports regarding the possible changes in density of post-synaptic targets of these terminals in the basal dendrites of CA3 neurons after overtraining in the MWM. The purpose of this work was to study the density of thorny excrescences (TE) and other dendritic spine types (stubby, thin, and mushroom) in the CA3 stratum oriens in animals overtrained in the MWM for three consecutive days and in animals trained for only one day. Seven days after MWM training, the animals were sacrificed, and their brains removed and processed for rapid Golgi staining to visualize the different types of dendritic protrusions. Our results revealed that the relative quantity of stubby, thin, and mushroom dendritic spines did not change, regardless of amount of training. However, a significant increase in the density of TE was detected in the overtrained animals. These results strongly suggest that spatial water maze overtraining induces an increased density of MF–TE connections, which might be functionally relevant for long-term spatial memory formation.Fil: Gómez Padilla, Eurídice. Universidad Autonoma de Queretaro.; MéxicoFil: Bello Medina, Paola C.. Universidad Nacional Autónoma de México; MéxicoFil: León Jacinto, Uriel. Universidad Nacional Autónoma de México; MéxicoFil: Orta Salazar, Erika. Universidad Nacional Autónoma de México; MéxicoFil: Quirarte, Gina L.. Universidad Nacional Autónoma de México; MéxicoFil: Ramirez Amaya, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Autonoma de Queretaro.; MéxicoFil: Prado Alcalá, Roberto A.. Universidad Nacional Autónoma de México; MéxicoFil: Díaz Cintra, Sofía. Universidad Nacional Autónoma de México; MéxicoElsevier Science2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140203Gómez Padilla, Eurídice; Bello Medina, Paola C.; León Jacinto, Uriel; Orta Salazar, Erika; Quirarte, Gina L.; et al.; Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons; Elsevier Science; Behavioural Brain Research; 379,; 2-2020; 1-230166-4328CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0166432819309490info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2019.112373info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:35:50Zoai:ri.conicet.gov.ar:11336/140203instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:50.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
title Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
spellingShingle Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
Gómez Padilla, Eurídice
CA3
DENDRITIC SPINES
HIPPOCAMPUS
MORRIS WATER MAZE
OVERTRAINING
THORNY EXCRESCENCES
title_short Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
title_full Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
title_fullStr Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
title_full_unstemmed Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
title_sort Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons
dc.creator.none.fl_str_mv Gómez Padilla, Eurídice
Bello Medina, Paola C.
León Jacinto, Uriel
Orta Salazar, Erika
Quirarte, Gina L.
Ramirez Amaya, Victor
Prado Alcalá, Roberto A.
Díaz Cintra, Sofía
author Gómez Padilla, Eurídice
author_facet Gómez Padilla, Eurídice
Bello Medina, Paola C.
León Jacinto, Uriel
Orta Salazar, Erika
Quirarte, Gina L.
Ramirez Amaya, Victor
Prado Alcalá, Roberto A.
Díaz Cintra, Sofía
author_role author
author2 Bello Medina, Paola C.
León Jacinto, Uriel
Orta Salazar, Erika
Quirarte, Gina L.
Ramirez Amaya, Victor
Prado Alcalá, Roberto A.
Díaz Cintra, Sofía
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CA3
DENDRITIC SPINES
HIPPOCAMPUS
MORRIS WATER MAZE
OVERTRAINING
THORNY EXCRESCENCES
topic CA3
DENDRITIC SPINES
HIPPOCAMPUS
MORRIS WATER MAZE
OVERTRAINING
THORNY EXCRESCENCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The hippocampus plays a fundamental role in spatial learning and memory. Dentate gyrus (DG) granular neurons project mainly to proximal apical dendrites of neurons in the CA3 stratum lucidum and also, to some extent, to the basal dendrites of CA3 pyramidal cells in the stratum oriens. The terminal specializations of DG neurons are the mossy fibers (MF), and these huge axon terminals show expansion in the CA3 stratum oriens after the animals undergo overtraining in the Morris Water Maze task (MWM). However, to our knowledge there are no reports regarding the possible changes in density of post-synaptic targets of these terminals in the basal dendrites of CA3 neurons after overtraining in the MWM. The purpose of this work was to study the density of thorny excrescences (TE) and other dendritic spine types (stubby, thin, and mushroom) in the CA3 stratum oriens in animals overtrained in the MWM for three consecutive days and in animals trained for only one day. Seven days after MWM training, the animals were sacrificed, and their brains removed and processed for rapid Golgi staining to visualize the different types of dendritic protrusions. Our results revealed that the relative quantity of stubby, thin, and mushroom dendritic spines did not change, regardless of amount of training. However, a significant increase in the density of TE was detected in the overtrained animals. These results strongly suggest that spatial water maze overtraining induces an increased density of MF–TE connections, which might be functionally relevant for long-term spatial memory formation.
Fil: Gómez Padilla, Eurídice. Universidad Autonoma de Queretaro.; México
Fil: Bello Medina, Paola C.. Universidad Nacional Autónoma de México; México
Fil: León Jacinto, Uriel. Universidad Nacional Autónoma de México; México
Fil: Orta Salazar, Erika. Universidad Nacional Autónoma de México; México
Fil: Quirarte, Gina L.. Universidad Nacional Autónoma de México; México
Fil: Ramirez Amaya, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Autonoma de Queretaro.; México
Fil: Prado Alcalá, Roberto A.. Universidad Nacional Autónoma de México; México
Fil: Díaz Cintra, Sofía. Universidad Nacional Autónoma de México; México
description The hippocampus plays a fundamental role in spatial learning and memory. Dentate gyrus (DG) granular neurons project mainly to proximal apical dendrites of neurons in the CA3 stratum lucidum and also, to some extent, to the basal dendrites of CA3 pyramidal cells in the stratum oriens. The terminal specializations of DG neurons are the mossy fibers (MF), and these huge axon terminals show expansion in the CA3 stratum oriens after the animals undergo overtraining in the Morris Water Maze task (MWM). However, to our knowledge there are no reports regarding the possible changes in density of post-synaptic targets of these terminals in the basal dendrites of CA3 neurons after overtraining in the MWM. The purpose of this work was to study the density of thorny excrescences (TE) and other dendritic spine types (stubby, thin, and mushroom) in the CA3 stratum oriens in animals overtrained in the MWM for three consecutive days and in animals trained for only one day. Seven days after MWM training, the animals were sacrificed, and their brains removed and processed for rapid Golgi staining to visualize the different types of dendritic protrusions. Our results revealed that the relative quantity of stubby, thin, and mushroom dendritic spines did not change, regardless of amount of training. However, a significant increase in the density of TE was detected in the overtrained animals. These results strongly suggest that spatial water maze overtraining induces an increased density of MF–TE connections, which might be functionally relevant for long-term spatial memory formation.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140203
Gómez Padilla, Eurídice; Bello Medina, Paola C.; León Jacinto, Uriel; Orta Salazar, Erika; Quirarte, Gina L.; et al.; Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons; Elsevier Science; Behavioural Brain Research; 379,; 2-2020; 1-23
0166-4328
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140203
identifier_str_mv Gómez Padilla, Eurídice; Bello Medina, Paola C.; León Jacinto, Uriel; Orta Salazar, Erika; Quirarte, Gina L.; et al.; Morris water maze overtraining increases the density of thorny excrescences in the basal dendrites of CA3 pyramidal neurons; Elsevier Science; Behavioural Brain Research; 379,; 2-2020; 1-23
0166-4328
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0166432819309490
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2019.112373
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083483391229952
score 13.22299

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