Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)

Autores
Galatro, Andrea Verónica; Puntarulo, Susana Ángela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Chloroplasts
Esr
No Generation
No2 −
Nos Like Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38904
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)Galatro, Andrea VerónicaPuntarulo, Susana ÁngelaChloroplastsEsrNo GenerationNo2 −Nos Like Activityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaHumana Press2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38904Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112978-1-4939-3600-71064-3745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-3600-7_9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:12:13Zoai:ri.conicet.gov.ar:11336/38904instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:12:13.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
title Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
spellingShingle Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
Galatro, Andrea Verónica
Chloroplasts
Esr
No Generation
No2 −
Nos Like Activity
title_short Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
title_full Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
title_fullStr Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
title_full_unstemmed Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
title_sort Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
dc.creator.none.fl_str_mv Galatro, Andrea Verónica
Puntarulo, Susana Ángela
author Galatro, Andrea Verónica
author_facet Galatro, Andrea Verónica
Puntarulo, Susana Ángela
author_role author
author2 Puntarulo, Susana Ángela
author2_role author
dc.subject.none.fl_str_mv Chloroplasts
Esr
No Generation
No2 −
Nos Like Activity
topic Chloroplasts
Esr
No Generation
No2 −
Nos Like Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
description Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38904
Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112
978-1-4939-3600-7
1064-3745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38904
identifier_str_mv Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112
978-1-4939-3600-7
1064-3745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-3600-7_9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781511997587456
score 12.982451

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