Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
- Autores
- Galatro, Andrea Verónica; Puntarulo, Susana Ángela
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina - Materia
-
Chloroplasts
Esr
No Generation
No2 −
Nos Like Activity - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/38904
Ver los metadatos del registro completo
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Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)Galatro, Andrea VerónicaPuntarulo, Susana ÁngelaChloroplastsEsrNo GenerationNo2 −Nos Like Activityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaHumana Press2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38904Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112978-1-4939-3600-71064-3745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-3600-7_9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:32Zoai:ri.conicet.gov.ar:11336/38904instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:32.407CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
title |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
spellingShingle |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) Galatro, Andrea Verónica Chloroplasts Esr No Generation No2 − Nos Like Activity |
title_short |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
title_full |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
title_fullStr |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
title_full_unstemmed |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
title_sort |
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) |
dc.creator.none.fl_str_mv |
Galatro, Andrea Verónica Puntarulo, Susana Ángela |
author |
Galatro, Andrea Verónica |
author_facet |
Galatro, Andrea Verónica Puntarulo, Susana Ángela |
author_role |
author |
author2 |
Puntarulo, Susana Ángela |
author2_role |
author |
dc.subject.none.fl_str_mv |
Chloroplasts Esr No Generation No2 − Nos Like Activity |
topic |
Chloroplasts Esr No Generation No2 − Nos Like Activity |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants. Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina |
description |
Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/38904 Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112 978-1-4939-3600-7 1064-3745 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/38904 |
identifier_str_mv |
Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112 978-1-4939-3600-7 1064-3745 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-3600-7_9 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_9 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Humana Press |
publisher.none.fl_str_mv |
Humana Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |