Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases

Autores
Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI  6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.
Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
MORACEAE
MILK CLOTTING
PLANT PROTEASE
ANTIOXIDANT ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271725
Acceder
id CONICETDig_67e4944ccbefcb52c3d7e2c9b1e053c3
oai_identifier_str oai:ri.conicet.gov.ar:11336/271725
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteasesCorrons, María AliciaBertucci, Juan IgnacioLiggieri, Constanza SilvinaLopez, Laura Maria IsabelBruno, Mariela AnahíMORACEAEMILK CLOTTINGPLANT PROTEASEANTIOXIDANT ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI  6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271725Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-1090023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643811004282info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2011.12.028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:09Zoai:ri.conicet.gov.ar:11336/271725instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:09.967CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
title Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
spellingShingle Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
Corrons, María Alicia
MORACEAE
MILK CLOTTING
PLANT PROTEASE
ANTIOXIDANT ACTIVITY
title_short Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
title_full Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
title_fullStr Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
title_full_unstemmed Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
title_sort Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
dc.creator.none.fl_str_mv Corrons, María Alicia
Bertucci, Juan Ignacio
Liggieri, Constanza Silvina
Lopez, Laura Maria Isabel
Bruno, Mariela Anahí
author Corrons, María Alicia
author_facet Corrons, María Alicia
Bertucci, Juan Ignacio
Liggieri, Constanza Silvina
Lopez, Laura Maria Isabel
Bruno, Mariela Anahí
author_role author
author2 Bertucci, Juan Ignacio
Liggieri, Constanza Silvina
Lopez, Laura Maria Isabel
Bruno, Mariela Anahí
author2_role author
author
author
author
dc.subject.none.fl_str_mv MORACEAE
MILK CLOTTING
PLANT PROTEASE
ANTIOXIDANT ACTIVITY
topic MORACEAE
MILK CLOTTING
PLANT PROTEASE
ANTIOXIDANT ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI  6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.
Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI  6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271725
Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-109
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271725
identifier_str_mv Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-109
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643811004282
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2011.12.028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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