Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases
- Autores
- Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI 6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.
Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina - Materia
-
MORACEAE
MILK CLOTTING
PLANT PROTEASE
ANTIOXIDANT ACTIVITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/271725
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Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteasesCorrons, María AliciaBertucci, Juan IgnacioLiggieri, Constanza SilvinaLopez, Laura Maria IsabelBruno, Mariela AnahíMORACEAEMILK CLOTTINGPLANT PROTEASEANTIOXIDANT ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI 6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods.Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271725Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-1090023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643811004282info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2011.12.028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:23Zoai:ri.conicet.gov.ar:11336/271725instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:24.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
title |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
spellingShingle |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases Corrons, María Alicia MORACEAE MILK CLOTTING PLANT PROTEASE ANTIOXIDANT ACTIVITY |
title_short |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
title_full |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
title_fullStr |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
title_full_unstemmed |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
title_sort |
Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases |
dc.creator.none.fl_str_mv |
Corrons, María Alicia Bertucci, Juan Ignacio Liggieri, Constanza Silvina Lopez, Laura Maria Isabel Bruno, Mariela Anahí |
author |
Corrons, María Alicia |
author_facet |
Corrons, María Alicia Bertucci, Juan Ignacio Liggieri, Constanza Silvina Lopez, Laura Maria Isabel Bruno, Mariela Anahí |
author_role |
author |
author2 |
Bertucci, Juan Ignacio Liggieri, Constanza Silvina Lopez, Laura Maria Isabel Bruno, Mariela Anahí |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
MORACEAE MILK CLOTTING PLANT PROTEASE ANTIOXIDANT ACTIVITY |
topic |
MORACEAE MILK CLOTTING PLANT PROTEASE ANTIOXIDANT ACTIVITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI 6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods. Fil: Corrons, María Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Bertucci, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina Fil: Liggieri, Constanza Silvina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina |
description |
A crude extract named pomiferin was obtained from latex of fruits of Maclura pomifera (Raf.) Schneid (Moraceae), containing serine endopeptidases. The caseinolytic activity was 14.1 +/_ 0.8 Ucas/mL and the protein concentration was 1.5 +/- 0.1 mg/mL. Isoelectrofocusing of pomiferin followed by zymogram analysis showed several bands (pI 3.7 - 9.2) of which those of pI 6.0 had a high proteolytic activity. The highest esterase activity was achieved on Ala, Gly and Leu derivatives of N-alfa-CBZ-pnitrophenyl amino acid esters. In milk clotting assays pomiferin showed a clotting strenght similar to that of chymosin standard (50 IMCU/mL), and clots presented stable consistency over time. A linear dependence was presented between the clotting time and the enzyme dilution.Clotting activity had increased significantly until 25 mmol/L of CaCl2 content. Wheys obtained using pomiferin and chymosin as clotting agents showed specific peptide profiles on tricine SDS-PAGE gels. Antioxidant activity and ACE inhibitory activity of filtered whey obtained with pomiferin were 57.4 +/- 0.4 % and 11 +/- 2 %, respectively. Both activities could be attributed to small peptides generated by action of proteases on milk proteins during clotting, therefore this whey could be used as ingredient in the manufacture of functional foods. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/271725 Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-109 0023-6438 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/271725 |
identifier_str_mv |
Corrons, María Alicia; Bertucci, Juan Ignacio; Liggieri, Constanza Silvina; Lopez, Laura Maria Isabel; Bruno, Mariela Anahí; Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases; Elsevier Science; LWT - Food Science and Technology; 47; 1; 1-2012; 103-109 0023-6438 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643811004282 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2011.12.028 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614240395067392 |
score |
13.070432 |