Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis

Autores
Flores Hernández, Emmanuel; Gonzalez, Maria Elisa; Alvarado Guitron, Paulina; Jasso Robles, Francisco Ignacio; Ovando Vázquez, Cesaré; Jiménez Bremont, Juan Francisco; Cavar Zeljkovic, Sanja; Ulbrichová, Markéta; De Diego, Nuria; Rodríguez y Domínguez Kessler, Margarita
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the Arabidopsis thaliana Polyamine Uptake Transporters gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the put2-1 mutant abolished systemic acquired resistance while enhancing basal resistance to Pseudomonas syringae pv. tomato DC3000. In contrast, the 35S::PUT2 overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the put2-1 mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the put2-1 mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in put2-1, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the put2-1 mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in Arabidopsis, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.
Fil: Flores Hernández, Emmanuel. Universidad Autónoma de San Luis Potosí; México
Fil: Gonzalez, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Alvarado Guitron, Paulina. Universidad Autónoma de San Luis Potosí; México
Fil: Jasso Robles, Francisco Ignacio. Palacky University Olomouc (upol);
Fil: Ovando Vázquez, Cesaré. Instituto Potosino de Investigación Científica y Tecnológica; México
Fil: Jiménez Bremont, Juan Francisco. Instituto Potosino de Investigación Científica y Tecnológica; México
Fil: Cavar Zeljkovic, Sanja. Palacky University Olomouc (upol);
Fil: Ulbrichová, Markéta. Palacky University Olomouc (upol);
Fil: De Diego, Nuria. Palacky University Olomouc (upol);
Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Autónoma de San Luis Potosí; México
Materia
PUTRESCINE
POLYAMINE TRANSPORT
REACTIVE OXYGEN SPECIES
SALICYLIC ACID
SYSTEMIC ACQUIRED RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/282633
Acceder
id CONICETDig_67ac9b05d991c5fe4e2ac229e3a20083
oai_identifier_str oai:ri.conicet.gov.ar:11336/282633
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in ArabidopsisFlores Hernández, EmmanuelGonzalez, Maria ElisaAlvarado Guitron, PaulinaJasso Robles, Francisco IgnacioOvando Vázquez, CesaréJiménez Bremont, Juan FranciscoCavar Zeljkovic, SanjaUlbrichová, MarkétaDe Diego, NuriaRodríguez y Domínguez Kessler, MargaritaPUTRESCINEPOLYAMINE TRANSPORTREACTIVE OXYGEN SPECIESSALICYLIC ACIDSYSTEMIC ACQUIRED RESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the Arabidopsis thaliana Polyamine Uptake Transporters gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the put2-1 mutant abolished systemic acquired resistance while enhancing basal resistance to Pseudomonas syringae pv. tomato DC3000. In contrast, the 35S::PUT2 overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the put2-1 mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the put2-1 mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in put2-1, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the put2-1 mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in Arabidopsis, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.Fil: Flores Hernández, Emmanuel. Universidad Autónoma de San Luis Potosí; MéxicoFil: Gonzalez, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Alvarado Guitron, Paulina. Universidad Autónoma de San Luis Potosí; MéxicoFil: Jasso Robles, Francisco Ignacio. Palacky University Olomouc (upol);Fil: Ovando Vázquez, Cesaré. Instituto Potosino de Investigación Científica y Tecnológica; MéxicoFil: Jiménez Bremont, Juan Francisco. Instituto Potosino de Investigación Científica y Tecnológica; MéxicoFil: Cavar Zeljkovic, Sanja. Palacky University Olomouc (upol);Fil: Ulbrichová, Markéta. Palacky University Olomouc (upol);Fil: De Diego, Nuria. Palacky University Olomouc (upol);Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Autónoma de San Luis Potosí; MéxicoElsevier2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/282633Flores Hernández, Emmanuel; Gonzalez, Maria Elisa; Alvarado Guitron, Paulina; Jasso Robles, Francisco Ignacio; Ovando Vázquez, Cesaré; et al.; Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis; Elsevier; Plant Stress; 18; 101046; 12-2025; 1-192667-064XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.stress.2025.101046info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2667064X25003148info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-28T12:11:45Zoai:ri.conicet.gov.ar:11336/282633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-28 12:11:45.819CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
title Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
spellingShingle Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
Flores Hernández, Emmanuel
PUTRESCINE
POLYAMINE TRANSPORT
REACTIVE OXYGEN SPECIES
SALICYLIC ACID
SYSTEMIC ACQUIRED RESISTANCE
title_short Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
title_full Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
title_fullStr Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
title_full_unstemmed Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
title_sort Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis
dc.creator.none.fl_str_mv Flores Hernández, Emmanuel
Gonzalez, Maria Elisa
Alvarado Guitron, Paulina
Jasso Robles, Francisco Ignacio
Ovando Vázquez, Cesaré
Jiménez Bremont, Juan Francisco
Cavar Zeljkovic, Sanja
Ulbrichová, Markéta
De Diego, Nuria
Rodríguez y Domínguez Kessler, Margarita
author Flores Hernández, Emmanuel
author_facet Flores Hernández, Emmanuel
Gonzalez, Maria Elisa
Alvarado Guitron, Paulina
Jasso Robles, Francisco Ignacio
Ovando Vázquez, Cesaré
Jiménez Bremont, Juan Francisco
Cavar Zeljkovic, Sanja
Ulbrichová, Markéta
De Diego, Nuria
Rodríguez y Domínguez Kessler, Margarita
author_role author
author2 Gonzalez, Maria Elisa
Alvarado Guitron, Paulina
Jasso Robles, Francisco Ignacio
Ovando Vázquez, Cesaré
Jiménez Bremont, Juan Francisco
Cavar Zeljkovic, Sanja
Ulbrichová, Markéta
De Diego, Nuria
Rodríguez y Domínguez Kessler, Margarita
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PUTRESCINE
POLYAMINE TRANSPORT
REACTIVE OXYGEN SPECIES
SALICYLIC ACID
SYSTEMIC ACQUIRED RESISTANCE
topic PUTRESCINE
POLYAMINE TRANSPORT
REACTIVE OXYGEN SPECIES
SALICYLIC ACID
SYSTEMIC ACQUIRED RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the Arabidopsis thaliana Polyamine Uptake Transporters gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the put2-1 mutant abolished systemic acquired resistance while enhancing basal resistance to Pseudomonas syringae pv. tomato DC3000. In contrast, the 35S::PUT2 overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the put2-1 mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the put2-1 mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in put2-1, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the put2-1 mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in Arabidopsis, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.
Fil: Flores Hernández, Emmanuel. Universidad Autónoma de San Luis Potosí; México
Fil: Gonzalez, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Alvarado Guitron, Paulina. Universidad Autónoma de San Luis Potosí; México
Fil: Jasso Robles, Francisco Ignacio. Palacky University Olomouc (upol);
Fil: Ovando Vázquez, Cesaré. Instituto Potosino de Investigación Científica y Tecnológica; México
Fil: Jiménez Bremont, Juan Francisco. Instituto Potosino de Investigación Científica y Tecnológica; México
Fil: Cavar Zeljkovic, Sanja. Palacky University Olomouc (upol);
Fil: Ulbrichová, Markéta. Palacky University Olomouc (upol);
Fil: De Diego, Nuria. Palacky University Olomouc (upol);
Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Autónoma de San Luis Potosí; México
description The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the Arabidopsis thaliana Polyamine Uptake Transporters gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the put2-1 mutant abolished systemic acquired resistance while enhancing basal resistance to Pseudomonas syringae pv. tomato DC3000. In contrast, the 35S::PUT2 overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the put2-1 mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the put2-1 mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in put2-1, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the put2-1 mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in Arabidopsis, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/282633
Flores Hernández, Emmanuel; Gonzalez, Maria Elisa; Alvarado Guitron, Paulina; Jasso Robles, Francisco Ignacio; Ovando Vázquez, Cesaré; et al.; Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis; Elsevier; Plant Stress; 18; 101046; 12-2025; 1-19
2667-064X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/282633
identifier_str_mv Flores Hernández, Emmanuel; Gonzalez, Maria Elisa; Alvarado Guitron, Paulina; Jasso Robles, Francisco Ignacio; Ovando Vázquez, Cesaré; et al.; Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis; Elsevier; Plant Stress; 18; 101046; 12-2025; 1-19
2667-064X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stress.2025.101046
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2667064X25003148
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.039084

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