Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification

Autores
Bolognese, Belén; Nerli, Bibiana Beatriz; Picó, Guillermo Alfredo
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (K r) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.
Fil: Bolognese, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
ETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMER
MALTODEXTRIN
PARTITIONING
TEMPERATURE-INDUCED TWO-PHASE SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95223
Acceder
id CONICETDig_6797402c77af71840cdd884dcfa6a834
oai_identifier_str oai:ri.conicet.gov.ar:11336/95223
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purificationBolognese, BelénNerli, Bibiana BeatrizPicó, Guillermo AlfredoETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMERMALTODEXTRINPARTITIONINGTEMPERATURE-INDUCED TWO-PHASE SYSTEMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (K r) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.Fil: Bolognese, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaElsevier Science2005-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95223Bolognese, Belén; Nerli, Bibiana Beatriz; Picó, Guillermo Alfredo; Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification; Elsevier Science; Journal of Chromatography B; 814; 2; 1-2005; 347-3530378-43471570-0232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023204008669info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2004.10.057info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:46Zoai:ri.conicet.gov.ar:11336/95223instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:47.262CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
title Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
spellingShingle Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
Bolognese, Belén
ETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMER
MALTODEXTRIN
PARTITIONING
TEMPERATURE-INDUCED TWO-PHASE SYSTEM
title_short Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
title_full Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
title_fullStr Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
title_full_unstemmed Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
title_sort Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
dc.creator.none.fl_str_mv Bolognese, Belén
Nerli, Bibiana Beatriz
Picó, Guillermo Alfredo
author Bolognese, Belén
author_facet Bolognese, Belén
Nerli, Bibiana Beatriz
Picó, Guillermo Alfredo
author_role author
author2 Nerli, Bibiana Beatriz
Picó, Guillermo Alfredo
author2_role author
author
dc.subject.none.fl_str_mv ETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMER
MALTODEXTRIN
PARTITIONING
TEMPERATURE-INDUCED TWO-PHASE SYSTEM
topic ETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMER
MALTODEXTRIN
PARTITIONING
TEMPERATURE-INDUCED TWO-PHASE SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (K r) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.
Fil: Bolognese, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (K r) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.
publishDate 2005
dc.date.none.fl_str_mv 2005-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95223
Bolognese, Belén; Nerli, Bibiana Beatriz; Picó, Guillermo Alfredo; Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification; Elsevier Science; Journal of Chromatography B; 814; 2; 1-2005; 347-353
0378-4347
1570-0232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95223
identifier_str_mv Bolognese, Belén; Nerli, Bibiana Beatriz; Picó, Guillermo Alfredo; Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification; Elsevier Science; Journal of Chromatography B; 814; 2; 1-2005; 347-353
0378-4347
1570-0232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023204008669
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2004.10.057
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270093914931200
score 13.13397

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