New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates

Autores
Martino, Florencia; Petroni, Alejandro; Menocal, María Alejandra; Corso, Alejandra; Melano, Roberto; Faccone, Diego Francisco
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteriaincluding carbapenem-resistant Enterobacterales. There are ten mcr genes described in differentGram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far themost frequent combination. In Argentina, mcr-1 gene was characterized only on plasmidsbelonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboringplasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolatescarrying the mcr-1 gene were sequenced using next generation technologies. Threeisolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226,ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1), IncX4(3), IncHI2/2A (3) and a hybrid IncFIA/HI1A/HI1B (1) plasmid. Plasmids belonging to theIncI2 (n = 1) and IncX4 (n = 3) groups showed high similarity with previously described plasmids.Two IncHI2/HI2A plasmids, showed high identity between them, while the third,showed several differences including additional resistance genes like tet(A) and floR. OneIncFIA/H1A/H1B hybrid plasmid was characterized, highly similar to pSRC27-H, a prototypeplasmid lacking mcr genes. mcr-1.5 variant was found in four plasmids with three differentInc groups: IncI2, IncHI2/HI2A and the hybrid FIA/HI1A/HI1B plasmid. mcr-1.5 variant isalmost exclusively described in our country and with a high frequency. In addition, six E. coliisolates carried three allelic variants codifying for CTX-M-type extended-spectrum-β-lactamases:blaCTX-M-2 (3), blaCTX-M-65 (2), and blaCTX-M-14 (1). It is the first description of mcr-1harboring plasmids different to IncI2 group in our country. These results represents newinsights about mcr-1 harboring plasmids recovered from E. coli human samples from Argentina,showing different plasmid backbones and resistance gene combinations.
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Menocal, María Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Melano, Roberto. Public Health Ontario.; Canadá
Fil: Faccone, Diego Francisco. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
MAR-1
COLISTIN
ESCHERICHIA COLI
PLASMID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230589
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230589
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolatesMartino, FlorenciaPetroni, AlejandroMenocal, María AlejandraCorso, AlejandraMelano, RobertoFaccone, Diego FranciscoMAR-1COLISTINESCHERICHIA COLIPLASMIDhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteriaincluding carbapenem-resistant Enterobacterales. There are ten mcr genes described in differentGram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far themost frequent combination. In Argentina, mcr-1 gene was characterized only on plasmidsbelonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboringplasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolatescarrying the mcr-1 gene were sequenced using next generation technologies. Threeisolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226,ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1), IncX4(3), IncHI2/2A (3) and a hybrid IncFIA/HI1A/HI1B (1) plasmid. Plasmids belonging to theIncI2 (n = 1) and IncX4 (n = 3) groups showed high similarity with previously described plasmids.Two IncHI2/HI2A plasmids, showed high identity between them, while the third,showed several differences including additional resistance genes like tet(A) and floR. OneIncFIA/H1A/H1B hybrid plasmid was characterized, highly similar to pSRC27-H, a prototypeplasmid lacking mcr genes. mcr-1.5 variant was found in four plasmids with three differentInc groups: IncI2, IncHI2/HI2A and the hybrid FIA/HI1A/HI1B plasmid. mcr-1.5 variant isalmost exclusively described in our country and with a high frequency. In addition, six E. coliisolates carried three allelic variants codifying for CTX-M-type extended-spectrum-β-lactamases:blaCTX-M-2 (3), blaCTX-M-65 (2), and blaCTX-M-14 (1). It is the first description of mcr-1harboring plasmids different to IncI2 group in our country. These results represents newinsights about mcr-1 harboring plasmids recovered from E. coli human samples from Argentina,showing different plasmid backbones and resistance gene combinations.Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Menocal, María Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Melano, Roberto. Public Health Ontario.; CanadáFil: Faccone, Diego Francisco. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPublic Library of Science2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230589Martino, Florencia; Petroni, Alejandro; Menocal, María Alejandra ; Corso, Alejandra; Melano, Roberto; et al.; New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates; Public Library of Science; Plos One; 19; 2-2024; 1-121932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0294820info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0294820info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:31Zoai:ri.conicet.gov.ar:11336/230589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:32.252CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
title New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
spellingShingle New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
Martino, Florencia
MAR-1
COLISTIN
ESCHERICHIA COLI
PLASMID
title_short New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
title_full New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
title_fullStr New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
title_full_unstemmed New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
title_sort New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates
dc.creator.none.fl_str_mv Martino, Florencia
Petroni, Alejandro
Menocal, María Alejandra
Corso, Alejandra
Melano, Roberto
Faccone, Diego Francisco
author Martino, Florencia
author_facet Martino, Florencia
Petroni, Alejandro
Menocal, María Alejandra
Corso, Alejandra
Melano, Roberto
Faccone, Diego Francisco
author_role author
author2 Petroni, Alejandro
Menocal, María Alejandra
Corso, Alejandra
Melano, Roberto
Faccone, Diego Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MAR-1
COLISTIN
ESCHERICHIA COLI
PLASMID
topic MAR-1
COLISTIN
ESCHERICHIA COLI
PLASMID
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteriaincluding carbapenem-resistant Enterobacterales. There are ten mcr genes described in differentGram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far themost frequent combination. In Argentina, mcr-1 gene was characterized only on plasmidsbelonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboringplasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolatescarrying the mcr-1 gene were sequenced using next generation technologies. Threeisolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226,ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1), IncX4(3), IncHI2/2A (3) and a hybrid IncFIA/HI1A/HI1B (1) plasmid. Plasmids belonging to theIncI2 (n = 1) and IncX4 (n = 3) groups showed high similarity with previously described plasmids.Two IncHI2/HI2A plasmids, showed high identity between them, while the third,showed several differences including additional resistance genes like tet(A) and floR. OneIncFIA/H1A/H1B hybrid plasmid was characterized, highly similar to pSRC27-H, a prototypeplasmid lacking mcr genes. mcr-1.5 variant was found in four plasmids with three differentInc groups: IncI2, IncHI2/HI2A and the hybrid FIA/HI1A/HI1B plasmid. mcr-1.5 variant isalmost exclusively described in our country and with a high frequency. In addition, six E. coliisolates carried three allelic variants codifying for CTX-M-type extended-spectrum-β-lactamases:blaCTX-M-2 (3), blaCTX-M-65 (2), and blaCTX-M-14 (1). It is the first description of mcr-1harboring plasmids different to IncI2 group in our country. These results represents newinsights about mcr-1 harboring plasmids recovered from E. coli human samples from Argentina,showing different plasmid backbones and resistance gene combinations.
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Petroni, Alejandro. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Menocal, María Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Melano, Roberto. Public Health Ontario.; Canadá
Fil: Faccone, Diego Francisco. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Mobile colistin resistance (mcr) genes were described recently in Gram-negative bacteriaincluding carbapenem-resistant Enterobacterales. There are ten mcr genes described in differentGram-negative bacteria, however, Escherichia coli harboring mcr-1 gene is by far themost frequent combination. In Argentina, mcr-1 gene was characterized only on plasmidsbelonging to IncI2 group. The aim of this work was to get new insights of mcr-1-harboringplasmids from E. coli. Eight E. coli isolates from a larger collection of 192 clinical E. coli isolatescarrying the mcr-1 gene were sequenced using next generation technologies. Threeisolates belonged to ST131 high-risk clone, and five to single ST, ST38, ST46, ST226,ST224, and ST405. Eight diverse mcr-1-harboring plasmids were analyzed: IncI2 (1), IncX4(3), IncHI2/2A (3) and a hybrid IncFIA/HI1A/HI1B (1) plasmid. Plasmids belonging to theIncI2 (n = 1) and IncX4 (n = 3) groups showed high similarity with previously described plasmids.Two IncHI2/HI2A plasmids, showed high identity between them, while the third,showed several differences including additional resistance genes like tet(A) and floR. OneIncFIA/H1A/H1B hybrid plasmid was characterized, highly similar to pSRC27-H, a prototypeplasmid lacking mcr genes. mcr-1.5 variant was found in four plasmids with three differentInc groups: IncI2, IncHI2/HI2A and the hybrid FIA/HI1A/HI1B plasmid. mcr-1.5 variant isalmost exclusively described in our country and with a high frequency. In addition, six E. coliisolates carried three allelic variants codifying for CTX-M-type extended-spectrum-β-lactamases:blaCTX-M-2 (3), blaCTX-M-65 (2), and blaCTX-M-14 (1). It is the first description of mcr-1harboring plasmids different to IncI2 group in our country. These results represents newinsights about mcr-1 harboring plasmids recovered from E. coli human samples from Argentina,showing different plasmid backbones and resistance gene combinations.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230589
Martino, Florencia; Petroni, Alejandro; Menocal, María Alejandra ; Corso, Alejandra; Melano, Roberto; et al.; New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates; Public Library of Science; Plos One; 19; 2-2024; 1-12
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230589
identifier_str_mv Martino, Florencia; Petroni, Alejandro; Menocal, María Alejandra ; Corso, Alejandra; Melano, Roberto; et al.; New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates; Public Library of Science; Plos One; 19; 2-2024; 1-12
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0294820
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0294820
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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