Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model

Autores
de Gregorio, Vanessa Elizabeth; Carino, Silvia; Valdéz, Juan Carlos; Castillo, Silvio Eugenio; de Moreno, Maria Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Immunotherapy by using non-specific vaccines has proven to be effective in experimental animal models and also in patients suffering cancer. In the present work, the effectiveness of this immunotherapy was evaluated using a murine breast cancer model and a polymicrobial vaccine. Methodology/Principal: Mice bearing injected with tumor cells from the spontaneous syngeneic mammary adeno carcinoma M3 were used as breast cancer model. The immune adjuvant effect of the vaccine was analyzed in comparison or in the presence of doxorubicin. Tumor volume was calculated. Tumor, spleen and lymph nodes were processed for histological observations and to determinate the percentage of CD11c (+) cells. Results: Mice treated with the vaccine or with doxorubicin decreased tumor growth, with less tumor cell invasion. Tumor tissues showed decreased necrosis and nuclear areas in mice that received any of the treatments under study, compared with the control group. The benefits of vaccination were associated to stimulation of the immune response. More hyperplasia of the red and white pulp, and increased marginal megakaryocytes were observed in the spleens from mice treated with the vaccine. CD11c (+) cells did not increase  in tumor draining nodes by the immune stimulating effect of the vaccine; however,  his treatment increased CD11c (+) cells in the spleen and tumor. Conclusions: A non-specific vaccine used as immunotherapy can be used to prolong an equilibrium phase in  he tumor growth, similar to the doxorubicin treatment used in the model, although by different mechanisms. Non synergic effect was observed in the group treated with both vaccine and doxorubicin (low dose). Future studies should be necessary to evaluate the correct combination of vaccination and cytotoxic drug can be used for this purpose and to achieve increased activated dendritic cells in the tumor draining lymph nodes.
Fil: de Gregorio, Vanessa Elizabeth. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
Fil: Castillo, Silvio Eugenio. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
BREAST CANCER
IMMUNE RESPONSE
VACCINE
DENDRITIC CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29304
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29304
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer modelde Gregorio, Vanessa ElizabethCarino, SilviaValdéz, Juan CarlosCastillo, Silvio Eugeniode Moreno, Maria AlejandraBREAST CANCERIMMUNE RESPONSEVACCINEDENDRITIC CELLShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Immunotherapy by using non-specific vaccines has proven to be effective in experimental animal models and also in patients suffering cancer. In the present work, the effectiveness of this immunotherapy was evaluated using a murine breast cancer model and a polymicrobial vaccine. Methodology/Principal: Mice bearing injected with tumor cells from the spontaneous syngeneic mammary adeno carcinoma M3 were used as breast cancer model. The immune adjuvant effect of the vaccine was analyzed in comparison or in the presence of doxorubicin. Tumor volume was calculated. Tumor, spleen and lymph nodes were processed for histological observations and to determinate the percentage of CD11c (+) cells. Results: Mice treated with the vaccine or with doxorubicin decreased tumor growth, with less tumor cell invasion. Tumor tissues showed decreased necrosis and nuclear areas in mice that received any of the treatments under study, compared with the control group. The benefits of vaccination were associated to stimulation of the immune response. More hyperplasia of the red and white pulp, and increased marginal megakaryocytes were observed in the spleens from mice treated with the vaccine. CD11c (+) cells did not increase  in tumor draining nodes by the immune stimulating effect of the vaccine; however,  his treatment increased CD11c (+) cells in the spleen and tumor. Conclusions: A non-specific vaccine used as immunotherapy can be used to prolong an equilibrium phase in  he tumor growth, similar to the doxorubicin treatment used in the model, although by different mechanisms. Non synergic effect was observed in the group treated with both vaccine and doxorubicin (low dose). Future studies should be necessary to evaluate the correct combination of vaccination and cytotoxic drug can be used for this purpose and to achieve increased activated dendritic cells in the tumor draining lymph nodes.Fil: de Gregorio, Vanessa Elizabeth. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Valdéz, Juan Carlos. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; ArgentinaFil: Castillo, Silvio Eugenio. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaClinMed International Library2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29304de Gregorio, Vanessa Elizabeth; Carino, Silvia; Valdéz, Juan Carlos; Castillo, Silvio Eugenio; de Moreno, Maria Alejandra; Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model; ClinMed International Library; International Journal of Immunology and Immunotherapy; 3; 2; 12-2016; 1-6;025-0252378-3672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.23937/2378-3672/1410025info:eu-repo/semantics/altIdentifier/url/https://clinmedjournals.org/articles/ijii/international-journal-of-immunology-and-immunotherapy-ijii-3-025.pdfinfo:eu-repo/semantics/altIdentifier/url/https://clinmedjournals.org/articles/ijii/international-journal-of-immunology-and-immunotherapy-ijii-3-025.php?jid=ijiiinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:51Zoai:ri.conicet.gov.ar:11336/29304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:51.658CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
title Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
spellingShingle Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
de Gregorio, Vanessa Elizabeth
BREAST CANCER
IMMUNE RESPONSE
VACCINE
DENDRITIC CELLS
title_short Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
title_full Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
title_fullStr Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
title_full_unstemmed Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
title_sort Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model
dc.creator.none.fl_str_mv de Gregorio, Vanessa Elizabeth
Carino, Silvia
Valdéz, Juan Carlos
Castillo, Silvio Eugenio
de Moreno, Maria Alejandra
author de Gregorio, Vanessa Elizabeth
author_facet de Gregorio, Vanessa Elizabeth
Carino, Silvia
Valdéz, Juan Carlos
Castillo, Silvio Eugenio
de Moreno, Maria Alejandra
author_role author
author2 Carino, Silvia
Valdéz, Juan Carlos
Castillo, Silvio Eugenio
de Moreno, Maria Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
IMMUNE RESPONSE
VACCINE
DENDRITIC CELLS
topic BREAST CANCER
IMMUNE RESPONSE
VACCINE
DENDRITIC CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Immunotherapy by using non-specific vaccines has proven to be effective in experimental animal models and also in patients suffering cancer. In the present work, the effectiveness of this immunotherapy was evaluated using a murine breast cancer model and a polymicrobial vaccine. Methodology/Principal: Mice bearing injected with tumor cells from the spontaneous syngeneic mammary adeno carcinoma M3 were used as breast cancer model. The immune adjuvant effect of the vaccine was analyzed in comparison or in the presence of doxorubicin. Tumor volume was calculated. Tumor, spleen and lymph nodes were processed for histological observations and to determinate the percentage of CD11c (+) cells. Results: Mice treated with the vaccine or with doxorubicin decreased tumor growth, with less tumor cell invasion. Tumor tissues showed decreased necrosis and nuclear areas in mice that received any of the treatments under study, compared with the control group. The benefits of vaccination were associated to stimulation of the immune response. More hyperplasia of the red and white pulp, and increased marginal megakaryocytes were observed in the spleens from mice treated with the vaccine. CD11c (+) cells did not increase  in tumor draining nodes by the immune stimulating effect of the vaccine; however,  his treatment increased CD11c (+) cells in the spleen and tumor. Conclusions: A non-specific vaccine used as immunotherapy can be used to prolong an equilibrium phase in  he tumor growth, similar to the doxorubicin treatment used in the model, although by different mechanisms. Non synergic effect was observed in the group treated with both vaccine and doxorubicin (low dose). Future studies should be necessary to evaluate the correct combination of vaccination and cytotoxic drug can be used for this purpose and to achieve increased activated dendritic cells in the tumor draining lymph nodes.
Fil: de Gregorio, Vanessa Elizabeth. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Microbiologia. Cátedra de Inmunología; Argentina
Fil: Castillo, Silvio Eugenio. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description Background: Immunotherapy by using non-specific vaccines has proven to be effective in experimental animal models and also in patients suffering cancer. In the present work, the effectiveness of this immunotherapy was evaluated using a murine breast cancer model and a polymicrobial vaccine. Methodology/Principal: Mice bearing injected with tumor cells from the spontaneous syngeneic mammary adeno carcinoma M3 were used as breast cancer model. The immune adjuvant effect of the vaccine was analyzed in comparison or in the presence of doxorubicin. Tumor volume was calculated. Tumor, spleen and lymph nodes were processed for histological observations and to determinate the percentage of CD11c (+) cells. Results: Mice treated with the vaccine or with doxorubicin decreased tumor growth, with less tumor cell invasion. Tumor tissues showed decreased necrosis and nuclear areas in mice that received any of the treatments under study, compared with the control group. The benefits of vaccination were associated to stimulation of the immune response. More hyperplasia of the red and white pulp, and increased marginal megakaryocytes were observed in the spleens from mice treated with the vaccine. CD11c (+) cells did not increase  in tumor draining nodes by the immune stimulating effect of the vaccine; however,  his treatment increased CD11c (+) cells in the spleen and tumor. Conclusions: A non-specific vaccine used as immunotherapy can be used to prolong an equilibrium phase in  he tumor growth, similar to the doxorubicin treatment used in the model, although by different mechanisms. Non synergic effect was observed in the group treated with both vaccine and doxorubicin (low dose). Future studies should be necessary to evaluate the correct combination of vaccination and cytotoxic drug can be used for this purpose and to achieve increased activated dendritic cells in the tumor draining lymph nodes.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29304
de Gregorio, Vanessa Elizabeth; Carino, Silvia; Valdéz, Juan Carlos; Castillo, Silvio Eugenio; de Moreno, Maria Alejandra; Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model; ClinMed International Library; International Journal of Immunology and Immunotherapy; 3; 2; 12-2016; 1-6;025-025
2378-3672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29304
identifier_str_mv de Gregorio, Vanessa Elizabeth; Carino, Silvia; Valdéz, Juan Carlos; Castillo, Silvio Eugenio; de Moreno, Maria Alejandra; Non-specific vaccination exerts anti-tumor effect through CD11c (+) cells stimulation in a breast cancer model; ClinMed International Library; International Journal of Immunology and Immunotherapy; 3; 2; 12-2016; 1-6;025-025
2378-3672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.23937/2378-3672/1410025
info:eu-repo/semantics/altIdentifier/url/https://clinmedjournals.org/articles/ijii/international-journal-of-immunology-and-immunotherapy-ijii-3-025.pdf
info:eu-repo/semantics/altIdentifier/url/https://clinmedjournals.org/articles/ijii/international-journal-of-immunology-and-immunotherapy-ijii-3-025.php?jid=ijii
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv ClinMed International Library
publisher.none.fl_str_mv ClinMed International Library
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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