Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices

Autores
Mulko, Lucinda Emma; Rivarola, Claudia Rosana; Barbero, César Alfredo; Acevedo, Diego Fernando
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.
Fil: Mulko, Lucinda Emma. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Acevedo, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
BIOETHANOL PRODUCTION
HETEROGENEOUS CATALYSTS
REUSABLE CATALYST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179950
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matricesMulko, Lucinda EmmaRivarola, Claudia RosanaBarbero, César AlfredoAcevedo, Diego FernandoBIOETHANOL PRODUCTIONHETEROGENEOUS CATALYSTSREUSABLE CATALYSThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.Fil: Mulko, Lucinda Emma. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Acevedo, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaElsevier Science2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179950Mulko, Lucinda Emma; Rivarola, Claudia Rosana; Barbero, César Alfredo; Acevedo, Diego Fernando; Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices; Elsevier Science; Journal of Biotechnology; 233; 8-2016; 56-650168-1656CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbiotec.2016.07.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:38Zoai:ri.conicet.gov.ar:11336/179950instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:38.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
title Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
spellingShingle Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
Mulko, Lucinda Emma
BIOETHANOL PRODUCTION
HETEROGENEOUS CATALYSTS
REUSABLE CATALYST
title_short Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
title_full Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
title_fullStr Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
title_full_unstemmed Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
title_sort Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices
dc.creator.none.fl_str_mv Mulko, Lucinda Emma
Rivarola, Claudia Rosana
Barbero, César Alfredo
Acevedo, Diego Fernando
author Mulko, Lucinda Emma
author_facet Mulko, Lucinda Emma
Rivarola, Claudia Rosana
Barbero, César Alfredo
Acevedo, Diego Fernando
author_role author
author2 Rivarola, Claudia Rosana
Barbero, César Alfredo
Acevedo, Diego Fernando
author2_role author
author
author
dc.subject.none.fl_str_mv BIOETHANOL PRODUCTION
HETEROGENEOUS CATALYSTS
REUSABLE CATALYST
topic BIOETHANOL PRODUCTION
HETEROGENEOUS CATALYSTS
REUSABLE CATALYST
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.
Fil: Mulko, Lucinda Emma. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Acevedo, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179950
Mulko, Lucinda Emma; Rivarola, Claudia Rosana; Barbero, César Alfredo; Acevedo, Diego Fernando; Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices; Elsevier Science; Journal of Biotechnology; 233; 8-2016; 56-65
0168-1656
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179950
identifier_str_mv Mulko, Lucinda Emma; Rivarola, Claudia Rosana; Barbero, César Alfredo; Acevedo, Diego Fernando; Bioethanol production by reusable Saccharomyces cerevisiaeimmobilized in a macroporous monolithic hydrogel matrices; Elsevier Science; Journal of Biotechnology; 233; 8-2016; 56-65
0168-1656
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbiotec.2016.07.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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