Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures

Autores
Giana, Fabián Eduardo; Bonetto, Fabian Jose; Bellotti, M. I.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present the design, construction and testing of a microelectrode array with spatial resolution which can be used for the detection of cancerous cells in a mixed cell culture by means of the impedance spectroscopy technique. Two different microelectrode diameters, namely 57 m and 211 m, were tested. A layer of an AuCu alloy was deposited between the glass substrate and the exterior Au layer, enhancing the adhesion of the microelectrodes to the substrate and at the same time showing lower impedance than the commonly used material Au. Characteristic features were extracted from the impedance measurements at different phases (growth, confluence, wound and healing) and used to feed a Linear Discriminant Analysis algorithm in order to discriminate between normal and cancerous cells. Relevant statistical tests were applied in the discrimination model for each phase. Finally, it was determined that the larger microelectrodes have a superior discriminant capacity: no incorrect classifications were obtained with microelectrodes measuring 211 m in diameter, while 23.5% false positives and 5.6% false negatives were obtained with 57 m microelectrodes.
Fil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bellotti, M. I.. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina
Materia
CANCER CELLS
ECIS
MICROELECTRODE ARRAY
MIXED CULTURE
SPATIAL RESOLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123867

id CONICETDig_66615d50cdf60e3497b6296eb02d1eb7
oai_identifier_str oai:ri.conicet.gov.ar:11336/123867
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed culturesGiana, Fabián EduardoBonetto, Fabian JoseBellotti, M. I.CANCER CELLSECISMICROELECTRODE ARRAYMIXED CULTURESPATIAL RESOLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We present the design, construction and testing of a microelectrode array with spatial resolution which can be used for the detection of cancerous cells in a mixed cell culture by means of the impedance spectroscopy technique. Two different microelectrode diameters, namely 57 m and 211 m, were tested. A layer of an AuCu alloy was deposited between the glass substrate and the exterior Au layer, enhancing the adhesion of the microelectrodes to the substrate and at the same time showing lower impedance than the commonly used material Au. Characteristic features were extracted from the impedance measurements at different phases (growth, confluence, wound and healing) and used to feed a Linear Discriminant Analysis algorithm in order to discriminate between normal and cancerous cells. Relevant statistical tests were applied in the discrimination model for each phase. Finally, it was determined that the larger microelectrodes have a superior discriminant capacity: no incorrect classifications were obtained with microelectrodes measuring 211 m in diameter, while 23.5% false positives and 5.6% false negatives were obtained with 57 m microelectrodes.Fil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bellotti, M. I.. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; ArgentinaIOP Publishing2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123867Giana, Fabián Eduardo; Bonetto, Fabian Jose; Bellotti, M. I.; Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures; IOP Publishing; Measurement Science & Technology (print); 31; 2; 10-2019; 1-90957-0233CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1361-6501/ab4d35info:eu-repo/semantics/altIdentifier/doi/10.1088/1361-6501/ab4d35info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:53Zoai:ri.conicet.gov.ar:11336/123867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:54.262CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
title Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
spellingShingle Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
Giana, Fabián Eduardo
CANCER CELLS
ECIS
MICROELECTRODE ARRAY
MIXED CULTURE
SPATIAL RESOLUTION
title_short Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
title_full Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
title_fullStr Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
title_full_unstemmed Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
title_sort Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures
dc.creator.none.fl_str_mv Giana, Fabián Eduardo
Bonetto, Fabian Jose
Bellotti, M. I.
author Giana, Fabián Eduardo
author_facet Giana, Fabián Eduardo
Bonetto, Fabian Jose
Bellotti, M. I.
author_role author
author2 Bonetto, Fabian Jose
Bellotti, M. I.
author2_role author
author
dc.subject.none.fl_str_mv CANCER CELLS
ECIS
MICROELECTRODE ARRAY
MIXED CULTURE
SPATIAL RESOLUTION
topic CANCER CELLS
ECIS
MICROELECTRODE ARRAY
MIXED CULTURE
SPATIAL RESOLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We present the design, construction and testing of a microelectrode array with spatial resolution which can be used for the detection of cancerous cells in a mixed cell culture by means of the impedance spectroscopy technique. Two different microelectrode diameters, namely 57 m and 211 m, were tested. A layer of an AuCu alloy was deposited between the glass substrate and the exterior Au layer, enhancing the adhesion of the microelectrodes to the substrate and at the same time showing lower impedance than the commonly used material Au. Characteristic features were extracted from the impedance measurements at different phases (growth, confluence, wound and healing) and used to feed a Linear Discriminant Analysis algorithm in order to discriminate between normal and cancerous cells. Relevant statistical tests were applied in the discrimination model for each phase. Finally, it was determined that the larger microelectrodes have a superior discriminant capacity: no incorrect classifications were obtained with microelectrodes measuring 211 m in diameter, while 23.5% false positives and 5.6% false negatives were obtained with 57 m microelectrodes.
Fil: Giana, Fabián Eduardo. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonetto, Fabian Jose. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bellotti, M. I.. Comisión Nacional de Energía Atómica. Gerencia de Área de Energía Nuclear. Gerencia de Ingeniería Nuclear (CAB). Laboratorio Cavitación y Biotecnología; Argentina
description We present the design, construction and testing of a microelectrode array with spatial resolution which can be used for the detection of cancerous cells in a mixed cell culture by means of the impedance spectroscopy technique. Two different microelectrode diameters, namely 57 m and 211 m, were tested. A layer of an AuCu alloy was deposited between the glass substrate and the exterior Au layer, enhancing the adhesion of the microelectrodes to the substrate and at the same time showing lower impedance than the commonly used material Au. Characteristic features were extracted from the impedance measurements at different phases (growth, confluence, wound and healing) and used to feed a Linear Discriminant Analysis algorithm in order to discriminate between normal and cancerous cells. Relevant statistical tests were applied in the discrimination model for each phase. Finally, it was determined that the larger microelectrodes have a superior discriminant capacity: no incorrect classifications were obtained with microelectrodes measuring 211 m in diameter, while 23.5% false positives and 5.6% false negatives were obtained with 57 m microelectrodes.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123867
Giana, Fabián Eduardo; Bonetto, Fabian Jose; Bellotti, M. I.; Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures; IOP Publishing; Measurement Science & Technology (print); 31; 2; 10-2019; 1-9
0957-0233
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123867
identifier_str_mv Giana, Fabián Eduardo; Bonetto, Fabian Jose; Bellotti, M. I.; Design and testing of a microelectrode array with spatial resolution for detection of cancerous cells in mixed cultures; IOP Publishing; Measurement Science & Technology (print); 31; 2; 10-2019; 1-9
0957-0233
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1361-6501/ab4d35
info:eu-repo/semantics/altIdentifier/doi/10.1088/1361-6501/ab4d35
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613294095073280
score 13.070432