β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation

Autores
Carpineti, L.; Martinez, María Julia; Pilosof, Ana Maria Renata; Perez, Oscar Edgardo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to build, to isolate and to characterize, core–shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core–shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core–shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core–shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations.
Fil: Carpineti, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Martinez, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Core-Shell Microparticles
Β-Lactoglobulin
Carboxymethylcellulose
Electrostatic Deposition
Lyophilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30401
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolationCarpineti, L.Martinez, María JuliaPilosof, Ana Maria RenataPerez, Oscar EdgardoCore-Shell MicroparticlesΒ-LactoglobulinCarboxymethylcelluloseElectrostatic DepositionLyophilizationhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The aim of this work was to build, to isolate and to characterize, core–shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core–shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core–shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core–shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations.Fil: Carpineti, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; ArgentinaFil: Martinez, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30401Carpineti, L.; Martinez, María Julia; Pilosof, Ana Maria Renata; Perez, Oscar Edgardo; β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation; Elsevier; Journal of Food Engineering; 131; 1-2014; 65-740260-8774CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfoodeng.2014.01.018info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0260877414000387info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:59Zoai:ri.conicet.gov.ar:11336/30401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:00.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
title β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
spellingShingle β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
Carpineti, L.
Core-Shell Microparticles
Β-Lactoglobulin
Carboxymethylcellulose
Electrostatic Deposition
Lyophilization
title_short β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
title_full β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
title_fullStr β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
title_full_unstemmed β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
title_sort β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation
dc.creator.none.fl_str_mv Carpineti, L.
Martinez, María Julia
Pilosof, Ana Maria Renata
Perez, Oscar Edgardo
author Carpineti, L.
author_facet Carpineti, L.
Martinez, María Julia
Pilosof, Ana Maria Renata
Perez, Oscar Edgardo
author_role author
author2 Martinez, María Julia
Pilosof, Ana Maria Renata
Perez, Oscar Edgardo
author2_role author
author
author
dc.subject.none.fl_str_mv Core-Shell Microparticles
Β-Lactoglobulin
Carboxymethylcellulose
Electrostatic Deposition
Lyophilization
topic Core-Shell Microparticles
Β-Lactoglobulin
Carboxymethylcellulose
Electrostatic Deposition
Lyophilization
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aim of this work was to build, to isolate and to characterize, core–shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core–shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core–shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core–shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations.
Fil: Carpineti, L.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
Fil: Martinez, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pilosof, Ana Maria Renata. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Oscar Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work was to build, to isolate and to characterize, core–shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core–shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core–shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core–shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30401
Carpineti, L.; Martinez, María Julia; Pilosof, Ana Maria Renata; Perez, Oscar Edgardo; β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation; Elsevier; Journal of Food Engineering; 131; 1-2014; 65-74
0260-8774
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30401
identifier_str_mv Carpineti, L.; Martinez, María Julia; Pilosof, Ana Maria Renata; Perez, Oscar Edgardo; β-Lactoglobulin–carboxymethylcellulose core–shell microparticles: Construction, characterization and isolation; Elsevier; Journal of Food Engineering; 131; 1-2014; 65-74
0260-8774
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfoodeng.2014.01.018
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0260877414000387
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268948457848832
score 13.13397

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