Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows

Autores
Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Aliverti, Florencia; Bandoni, Arnaldo Luis; Marchetti, Maria Laura; Mestorino, Olga Nora
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essential oil (EO) has been reported as a good antimicrobial against S. aureus. The aim of this work was to investigate, in vitro, the combined effect of EO and RIF to identify a synergic interaction against S. aureus, in order to obtain sufficient information for subsequent pharmacokinetic / pharmacodynamic studies. Minimum Inhibitory Concentration (MIC) for RIF, EO and combinations of this against S. aureus strains were determined at pH 7.4; 6.5 and 5.0, representing intracellular conditions where S. aureus is usually located. Fractional Inhibitory Concentration Index (FIC) and the index of antibacterial activity (E) were evaluated. The MIC of EO at pH 7.4 was 25-12.5 μL/mL and decreased with the acidity of the medium. RIF presented a high antimicrobial activity (0.032 μg/mL) against S. aureus regardless of pH conditions. Combining RIF with EO, we found a synergic effect. A mix of 0.004 μg/mL of RIF and 12.5 μL/mL of EO led to a virtual eradication effect against wild types strains at pH 7.4. Media acidification improves the EO/RIF activity, so EO would be a good adjuvant for RIF to treat staphylococcal infections and decrease antimicrobial resistance.
Fil: Buldain, Daniel Cornelio. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gortari Castillo, Lihuel. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Aliverti, Florencia. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Bandoni, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Materia
STAPHYLOCOCCUS AUREUS
DAIRY COWS
MELALECUA ARMILLARIS
ESSENTIAL OIL
RIFAXIMIN
CHECKERBOARD
SYNERGISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157765
Acceder
id CONICETDig_65f369ff755877b1ace8f4cefffd76a5
oai_identifier_str oai:ri.conicet.gov.ar:11336/157765
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cowsBuldain, Daniel CornelioGortari Castillo, LihuelBuchamer, Andrea VeronicaAliverti, FlorenciaBandoni, Arnaldo LuisMarchetti, Maria LauraMestorino, Olga NoraSTAPHYLOCOCCUS AUREUSDAIRY COWSMELALECUA ARMILLARISESSENTIAL OILRIFAXIMINCHECKERBOARDSYNERGISMhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essential oil (EO) has been reported as a good antimicrobial against S. aureus. The aim of this work was to investigate, in vitro, the combined effect of EO and RIF to identify a synergic interaction against S. aureus, in order to obtain sufficient information for subsequent pharmacokinetic / pharmacodynamic studies. Minimum Inhibitory Concentration (MIC) for RIF, EO and combinations of this against S. aureus strains were determined at pH 7.4; 6.5 and 5.0, representing intracellular conditions where S. aureus is usually located. Fractional Inhibitory Concentration Index (FIC) and the index of antibacterial activity (E) were evaluated. The MIC of EO at pH 7.4 was 25-12.5 μL/mL and decreased with the acidity of the medium. RIF presented a high antimicrobial activity (0.032 μg/mL) against S. aureus regardless of pH conditions. Combining RIF with EO, we found a synergic effect. A mix of 0.004 μg/mL of RIF and 12.5 μL/mL of EO led to a virtual eradication effect against wild types strains at pH 7.4. Media acidification improves the EO/RIF activity, so EO would be a good adjuvant for RIF to treat staphylococcal infections and decrease antimicrobial resistance.Fil: Buldain, Daniel Cornelio. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gortari Castillo, Lihuel. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Aliverti, Florencia. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Bandoni, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFrontiers Media2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157765Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Aliverti, Florencia; Bandoni, Arnaldo Luis; et al.; Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows; Frontiers Media; Frontiers in Veterinary Science; 7; 7-2020; 1-102297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.00344info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.00344/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:46Zoai:ri.conicet.gov.ar:11336/157765instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:46.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
title Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
spellingShingle Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
Buldain, Daniel Cornelio
STAPHYLOCOCCUS AUREUS
DAIRY COWS
MELALECUA ARMILLARIS
ESSENTIAL OIL
RIFAXIMIN
CHECKERBOARD
SYNERGISM
title_short Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
title_full Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
title_fullStr Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
title_full_unstemmed Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
title_sort Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows
dc.creator.none.fl_str_mv Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Veronica
Aliverti, Florencia
Bandoni, Arnaldo Luis
Marchetti, Maria Laura
Mestorino, Olga Nora
author Buldain, Daniel Cornelio
author_facet Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Veronica
Aliverti, Florencia
Bandoni, Arnaldo Luis
Marchetti, Maria Laura
Mestorino, Olga Nora
author_role author
author2 Gortari Castillo, Lihuel
Buchamer, Andrea Veronica
Aliverti, Florencia
Bandoni, Arnaldo Luis
Marchetti, Maria Laura
Mestorino, Olga Nora
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv STAPHYLOCOCCUS AUREUS
DAIRY COWS
MELALECUA ARMILLARIS
ESSENTIAL OIL
RIFAXIMIN
CHECKERBOARD
SYNERGISM
topic STAPHYLOCOCCUS AUREUS
DAIRY COWS
MELALECUA ARMILLARIS
ESSENTIAL OIL
RIFAXIMIN
CHECKERBOARD
SYNERGISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essential oil (EO) has been reported as a good antimicrobial against S. aureus. The aim of this work was to investigate, in vitro, the combined effect of EO and RIF to identify a synergic interaction against S. aureus, in order to obtain sufficient information for subsequent pharmacokinetic / pharmacodynamic studies. Minimum Inhibitory Concentration (MIC) for RIF, EO and combinations of this against S. aureus strains were determined at pH 7.4; 6.5 and 5.0, representing intracellular conditions where S. aureus is usually located. Fractional Inhibitory Concentration Index (FIC) and the index of antibacterial activity (E) were evaluated. The MIC of EO at pH 7.4 was 25-12.5 μL/mL and decreased with the acidity of the medium. RIF presented a high antimicrobial activity (0.032 μg/mL) against S. aureus regardless of pH conditions. Combining RIF with EO, we found a synergic effect. A mix of 0.004 μg/mL of RIF and 12.5 μL/mL of EO led to a virtual eradication effect against wild types strains at pH 7.4. Media acidification improves the EO/RIF activity, so EO would be a good adjuvant for RIF to treat staphylococcal infections and decrease antimicrobial resistance.
Fil: Buldain, Daniel Cornelio. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gortari Castillo, Lihuel. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Aliverti, Florencia. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Bandoni, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
description Staphylococcus aureus is the major subclinical mastitis-causing pathogen in dairy cows. In some European and Latin American countries rifaximin (RIF) is a commonly used therapy at drying off. Phytotherapeutics are alternatives for the treatment of infectious diseases. Melaleuca armillaris essential oil (EO) has been reported as a good antimicrobial against S. aureus. The aim of this work was to investigate, in vitro, the combined effect of EO and RIF to identify a synergic interaction against S. aureus, in order to obtain sufficient information for subsequent pharmacokinetic / pharmacodynamic studies. Minimum Inhibitory Concentration (MIC) for RIF, EO and combinations of this against S. aureus strains were determined at pH 7.4; 6.5 and 5.0, representing intracellular conditions where S. aureus is usually located. Fractional Inhibitory Concentration Index (FIC) and the index of antibacterial activity (E) were evaluated. The MIC of EO at pH 7.4 was 25-12.5 μL/mL and decreased with the acidity of the medium. RIF presented a high antimicrobial activity (0.032 μg/mL) against S. aureus regardless of pH conditions. Combining RIF with EO, we found a synergic effect. A mix of 0.004 μg/mL of RIF and 12.5 μL/mL of EO led to a virtual eradication effect against wild types strains at pH 7.4. Media acidification improves the EO/RIF activity, so EO would be a good adjuvant for RIF to treat staphylococcal infections and decrease antimicrobial resistance.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157765
Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Aliverti, Florencia; Bandoni, Arnaldo Luis; et al.; Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows; Frontiers Media; Frontiers in Veterinary Science; 7; 7-2020; 1-10
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157765
identifier_str_mv Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Aliverti, Florencia; Bandoni, Arnaldo Luis; et al.; Melaleuca armillaris essential oil in combination with rifaximin against Staphylococcus aureus isolated of dairy cows; Frontiers Media; Frontiers in Veterinary Science; 7; 7-2020; 1-10
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.00344
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.00344/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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