Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
- Autores
- Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; Avila, J. P.; Butti, M.; Abba, Martín Carlos
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.
Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; Colombia
Fil: Aristizabal, F.. Universidad Nacional de Colombia; Colombia
Fil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; Colombia
Fil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina - Materia
-
BIOSIMILAR
G-CSF
INNOVATOR
TRANSCRIPTOMICS
UMBILICAL CORD BLOOD - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/136376
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cellsÁvila Portillo, Luz MabelAristizabal, F.Perdomo, S.Riveros, A.Ospino, B.Avila, J. P.Butti, M.Abba, Martín CarlosBIOSIMILARG-CSFINNOVATORTRANSCRIPTOMICSUMBILICAL CORD BLOODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; ColombiaFil: Aristizabal, F.. Universidad Nacional de Colombia; ColombiaFil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; ColombiaFil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaSAGE Publications2020-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136376Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-71177-93221177-9322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/1177932220913307info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1177932220913307info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:47Zoai:ri.conicet.gov.ar:11336/136376instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:48.132CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
title |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
spellingShingle |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells Ávila Portillo, Luz Mabel BIOSIMILAR G-CSF INNOVATOR TRANSCRIPTOMICS UMBILICAL CORD BLOOD |
title_short |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
title_full |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
title_fullStr |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
title_full_unstemmed |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
title_sort |
Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells |
dc.creator.none.fl_str_mv |
Ávila Portillo, Luz Mabel Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, M. Abba, Martín Carlos |
author |
Ávila Portillo, Luz Mabel |
author_facet |
Ávila Portillo, Luz Mabel Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, M. Abba, Martín Carlos |
author_role |
author |
author2 |
Aristizabal, F. Perdomo, S. Riveros, A. Ospino, B. Avila, J. P. Butti, M. Abba, Martín Carlos |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
BIOSIMILAR G-CSF INNOVATOR TRANSCRIPTOMICS UMBILICAL CORD BLOOD |
topic |
BIOSIMILAR G-CSF INNOVATOR TRANSCRIPTOMICS UMBILICAL CORD BLOOD |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses. Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; Colombia Fil: Aristizabal, F.. Universidad Nacional de Colombia; Colombia Fil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; Colombia Fil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; Colombia Fil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; Colombia Fil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; Colombia Fil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina |
description |
Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-20 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/136376 Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-7 1177-9322 1177-9322 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/136376 |
identifier_str_mv |
Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-7 1177-9322 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1177/1177932220913307 info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1177932220913307 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
SAGE Publications |
publisher.none.fl_str_mv |
SAGE Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.22299 |