Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells

Autores
Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; Avila, J. P.; Butti, M.; Abba, Martín Carlos
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.
Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; Colombia
Fil: Aristizabal, F.. Universidad Nacional de Colombia; Colombia
Fil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; Colombia
Fil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
BIOSIMILAR
G-CSF
INNOVATOR
TRANSCRIPTOMICS
UMBILICAL CORD BLOOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/136376
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/136376
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cellsÁvila Portillo, Luz MabelAristizabal, F.Perdomo, S.Riveros, A.Ospino, B.Avila, J. P.Butti, M.Abba, Martín CarlosBIOSIMILARG-CSFINNOVATORTRANSCRIPTOMICSUMBILICAL CORD BLOODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; ColombiaFil: Aristizabal, F.. Universidad Nacional de Colombia; ColombiaFil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; ColombiaFil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaSAGE Publications2020-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136376Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-71177-93221177-9322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/1177932220913307info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1177932220913307info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:48:00Zoai:ri.conicet.gov.ar:11336/136376instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:48:00.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
title Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
spellingShingle Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
Ávila Portillo, Luz Mabel
BIOSIMILAR
G-CSF
INNOVATOR
TRANSCRIPTOMICS
UMBILICAL CORD BLOOD
title_short Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
title_full Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
title_fullStr Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
title_full_unstemmed Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
title_sort Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells
dc.creator.none.fl_str_mv Ávila Portillo, Luz Mabel
Aristizabal, F.
Perdomo, S.
Riveros, A.
Ospino, B.
Avila, J. P.
Butti, M.
Abba, Martín Carlos
author Ávila Portillo, Luz Mabel
author_facet Ávila Portillo, Luz Mabel
Aristizabal, F.
Perdomo, S.
Riveros, A.
Ospino, B.
Avila, J. P.
Butti, M.
Abba, Martín Carlos
author_role author
author2 Aristizabal, F.
Perdomo, S.
Riveros, A.
Ospino, B.
Avila, J. P.
Butti, M.
Abba, Martín Carlos
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOSIMILAR
G-CSF
INNOVATOR
TRANSCRIPTOMICS
UMBILICAL CORD BLOOD
topic BIOSIMILAR
G-CSF
INNOVATOR
TRANSCRIPTOMICS
UMBILICAL CORD BLOOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.
Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; Colombia
Fil: Aristizabal, F.. Universidad Nacional de Colombia; Colombia
Fil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; Colombia
Fil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; Colombia
Fil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/136376
Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-7
1177-9322
1177-9322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/136376
identifier_str_mv Ávila Portillo, Luz Mabel; Aristizabal, F.; Perdomo, S.; Riveros, A.; Ospino, B.; et al.; Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells; SAGE Publications; Bioinformatics and Biology Insights; 14; 20-3-2020; 1-7
1177-9322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/1177932220913307
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1177932220913307
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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