Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition

Autores
Sanchez Vasquez, Estefania; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3′UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a ‘sponge’ vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.
Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
Materia
DNA METHYLATION
EMT
MIGRATION
MIR-203
NEURAL CREST
PHF12
SNAIL2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117727
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117727
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transitionSanchez Vasquez, EstefaniaBronner, Marianne E.Strobl Mazulla, Pablo HernanDNA METHYLATIONEMTMIGRATIONMIR-203NEURAL CRESTPHF12SNAIL2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3′UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a ‘sponge’ vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); ArgentinaFil: Bronner, Marianne E.. California Institute of Technology; Estados UnidosFil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); ArgentinaCompany of Biologists2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117727Sanchez Vasquez, Estefania; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition; Company of Biologists; Development; 146; 7; 4-2019; 1-90950-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/lookup/doi/10.1242/dev.171017info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.171017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:17Zoai:ri.conicet.gov.ar:11336/117727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:17.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
title Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
spellingShingle Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
Sanchez Vasquez, Estefania
DNA METHYLATION
EMT
MIGRATION
MIR-203
NEURAL CREST
PHF12
SNAIL2
title_short Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
title_full Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
title_fullStr Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
title_full_unstemmed Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
title_sort Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition
dc.creator.none.fl_str_mv Sanchez Vasquez, Estefania
Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author Sanchez Vasquez, Estefania
author_facet Sanchez Vasquez, Estefania
Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author_role author
author2 Bronner, Marianne E.
Strobl Mazulla, Pablo Hernan
author2_role author
author
dc.subject.none.fl_str_mv DNA METHYLATION
EMT
MIGRATION
MIR-203
NEURAL CREST
PHF12
SNAIL2
topic DNA METHYLATION
EMT
MIGRATION
MIR-203
NEURAL CREST
PHF12
SNAIL2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3′UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a ‘sponge’ vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.
Fil: Sanchez Vasquez, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús) | Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas (subsede Chascomús); Argentina
description miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role in the context of neural crest development. During the epithelial-to-mesenchymal transition of neural crest cells to emigrate from the neural tube, miR-203 displays a reciprocal expression pattern with key regulators of neural crest delamination, Phf12 and Snail2, and interacts with their 3′UTRs. We show that ectopic maintenance of miR-203 inhibits neural crest migration in chick, whereas its functional inhibition using a ‘sponge’ vector or morpholinos promotes premature neural crest delamination. Bisulfite sequencing further shows that epigenetic repression of miR-203 is mediated by the de novo DNA methyltransferase DNMT3B, the recruitment of which to regulatory regions on the miR-203 locus is directed by SNAIL2 in a negative-feedback loop. These findings reveal an important role for miR-203 in an epigenetic-microRNA regulatory network that influences the timing of neural crest delamination.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117727
Sanchez Vasquez, Estefania; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition; Company of Biologists; Development; 146; 7; 4-2019; 1-9
0950-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117727
identifier_str_mv Sanchez Vasquez, Estefania; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Epigenetic inactivation of mir-203 as a key step in neural crest epithelial-to-mesenchymal transition; Company of Biologists; Development; 146; 7; 4-2019; 1-9
0950-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/lookup/doi/10.1242/dev.171017
info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.171017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083185985716224
score 13.22299

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